Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,678,604	G→T	A60S (GCA→TCA)	ydgH →	DUF1471 domain‑containing protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,678,604	0	G	T	100.0%	166.4 / NA	49	A60S (GCA→TCA)	ydgH	DUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/25);  total (24/25)

TGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTA  >  NC_000913/1678473‑1678739                                                                                                                                    |                                                                                                                                        tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATaaa                                                                                                                                 >  2:815795/1‑140 (MQ=255)  ggCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaag                                                                                                                                <  1:131230/140‑1 (MQ=255)      ttCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAgg                                                                                                                            >  1:204911/1‑140 (MQ=255)        ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCa                                                                                                                                       >  1:743418/1‑127 (MQ=255)        ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCa                                                                                                                                       >  1:119787/1‑127 (MQ=255)        ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTg                                                                                                                          >  1:675976/1‑140 (MQ=255)        ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTg                                                                                                                          <  2:18793/140‑1 (MQ=255)             ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                     >  2:227412/1‑140 (MQ=255)                    agcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCttttt                                                                                                              <  1:815795/140‑1 (MQ=255)                     gcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTa                                                                                                             >  1:851842/1‑140 (MQ=255)                      caACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAt                                                                                                            <  1:342116/140‑1 (MQ=255)                              cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                    <  2:406731/140‑1 (MQ=255)                              cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                    <  2:851842/140‑1 (MQ=255)                              cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                    <  1:164040/140‑1 (MQ=255)                                   cccGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAGGAAGGTGCCGCCTCTTTTTATGTTGTCGACACtt                                                                                               <  1:428117/139‑1 (MQ=255)                                   acCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACtt                                                                                               >  2:306399/1‑140 (MQ=255)                                   acCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACtt                                                                                               >  2:204031/1‑140 (MQ=255)                                      ggAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTg                                                                                            <  1:227412/140‑1 (MQ=255)                                      ggAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTg                                                                                            <  1:155363/140‑1 (MQ=255)                                               ggCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTa                                                                                   <  2:810927/140‑1 (MQ=255)                                                  aGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACa                                                                                >  1:451891/1‑140 (MQ=255)                                                       ttAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGt                                                                           <  1:18816/140‑1 (MQ=255)                                                                 tGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCAGATTTTGGTAACAGCGGTAACTGGCgtg                                                                 >  2:736018/1‑140 (MQ=255)                                                                       tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                           <  2:467825/140‑1 (MQ=255)                                                                        gtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGc                                                          <  1:663297/140‑1 (MQ=255)                                                                         tAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                         <  2:451891/140‑1 (MQ=255)                                                                            tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                      <  2:675976/140‑1 (MQ=255)                                                                            tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                      <  2:696959/140‑1 (MQ=255)                                                                            tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                      <  2:204911/140‑1 (MQ=255)                                                                            tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                      <  2:852722/140‑1 (MQ=255)                                                                            tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                      <  1:204031/140‑1 (MQ=255)                                                                            tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                      <  1:306399/140‑1 (MQ=255)                                                                                 aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTTGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                 >  2:897225/1‑140 (MQ=255)                                                                                 aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                      <  1:754348/135‑1 (MQ=255)                                                                                 aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                      >  2:754348/1‑135 (MQ=255)                                                                                 aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                 >  2:68170/1‑140 (MQ=255)                                                                                     gTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATaaa                                             >  1:343778/1‑140 (MQ=255)                                                                                        gTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGcc                                          >  1:722528/1‑140 (MQ=255)                                                                                                   aTTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCAGATGCTGaaaa                               >  1:794401/1‑140 (MQ=255)                                                                                                    ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                              >  1:918602/1‑140 (MQ=255)                                                                                                    ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                              >  2:783462/1‑140 (MQ=255)                                                                                                       gCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCa                           <  1:68170/140‑1 (MQ=255)                                                                                                           aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                       >  2:832498/1‑140 (MQ=255)                                                                                                                       cGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATc           >  1:894588/1‑140 (MQ=255)                                                                                                                          ttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTTGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgcg        >  1:447435/1‑140 (MQ=255)                                                                                                                          ttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgcg        <  2:497939/140‑1 (MQ=255)                                                                                                                          ttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgcg        >  2:706266/1‑140 (MQ=255)                                                                                                                            ctcGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTa      <  1:671907/140‑1 (MQ=255)                                                                                                                                tcgCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTa  >  2:537047/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                                        TGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTA  >  NC_000913/1678473‑1678739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑