Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,171,892 G→T Q328K (CAG→AAG)  gatD ← galactitol‑1‑phosphate 5‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,171,8920GT100.0% 148.2 / NA 44Q328K (CAG→AAG) gatDgalactitol‑1‑phosphate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/22);  total (22/22)

ACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATCCAACTGCCGATAA  >  NC_000913/2171763‑2172026
                                                                                                                                 |                                                                                                                                      
aCGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCtt                                                                                                                              >  1:80188/1‑140 (MQ=255)
    aGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCaa                                                                                                                          <  2:662611/140‑1 (MQ=255)
     gggggACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCaaa                                                                                                                         >  2:620956/1‑140 (MQ=255)
     gggggACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCaaa                                                                                                                         >  2:20265/1‑140 (MQ=255)
     gggggACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCaaa                                                                                                                         >  1:693505/1‑140 (MQ=255)
                ggTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACTAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGt                                                                                                              <  1:56795/140‑1 (MQ=255)
                ggTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGt                                                                                                              <  1:684874/140‑1 (MQ=255)
                           tGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAAt                                                                                                   >  2:660480/1‑140 (MQ=255)
                               ttGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCt                                                                                               <  1:527574/140‑1 (MQ=255)
                                tGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGCCTc                                                                                              <  2:505122/140‑1 (MQ=255)
                                       tACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCtt                                                                                       >  2:549378/1‑140 (MQ=255)
                                       tACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCtt                                                                                       >  1:688607/1‑140 (MQ=255)
                                           gggCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACt                                                                                   <  2:756139/140‑1 (MQ=255)
                                            ggCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACtt                                                                                  >  1:251454/1‑140 (MQ=255)
                                            ggCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACtt                                                                                  >  2:212275/1‑140 (MQ=255)
                                                 gCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGtt                                                                             >  2:428234/1‑140 (MQ=255)
                                                            gCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAAc                                                                  >  2:788319/1‑140 (MQ=255)
                                                             cccGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAAcc                                                                 >  1:441949/1‑140 (MQ=255)
                                                             cccGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAAcc                                                                 >  1:845772/1‑140 (MQ=255)
                                                                 cgGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCt                                                             <  1:82622/140‑1 (MQ=255)
                                                                     ttCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGcc                                                         <  1:900486/140‑1 (MQ=255)
                                                                      tCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCg                                                        >  2:644186/1‑140 (MQ=255)
                                                                      tCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCg                                                        <  2:251454/140‑1 (MQ=255)
                                                                              tGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCAc                                                >  1:31746/1‑140 (MQ=255)
                                                                                      acTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGccc                                        <  2:900510/140‑1 (MQ=255)
                                                                                           gCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGcc                                   <  1:644186/140‑1 (MQ=255)
                                                                                           gCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGcc                                   <  1:904031/140‑1 (MQ=255)
                                                                                            cccGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCa                                  <  2:31746/140‑1 (MQ=255)
                                                                                            cccGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCa                                  <  1:901941/140‑1 (MQ=255)
                                                                                            cccGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCa                                  <  2:45241/140‑1 (MQ=255)
                                                                                              cGGCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAg                                <  2:506340/140‑1 (MQ=255)
                                                                                               ggCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAgg                               >  1:818109/1‑140 (MQ=255)
                                                                                               ggCATAGCATTACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAgg                               >  1:644406/1‑140 (MQ=255)
                                                                                                        ttACGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGagta                      <  2:910945/140‑1 (MQ=255)
                                                                                                           cGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGtt                   >  1:947747/1‑140 (MQ=255)
                                                                                                           cGAGCGATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGtt                   >  2:755913/1‑140 (MQ=255)
                                                                                                               cGATGTCACGCACCGCCTTGGCGAAGCTTCCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATc               >  1:547499/1‑140 (MQ=255)
                                                                                                                gATGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATcc              >  2:732720/1‑140 (MQ=255)
                                                                                                                  tGTCACGCGCCGCCTTGGCGAAGCTTTAAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATCCaa            <  2:309663/140‑1 (MQ=255)
                                                                                                                  tGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATCCaa            <  1:755913/140‑1 (MQ=255)
                                                                                                                  tGTCACGCACCGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATCCaa            <  1:549378/140‑1 (MQ=255)
                                                                                                                           ccGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATCCAACTGCCGATa   <  2:577932/140‑1 (MQ=255)
                                                                                                                           ccGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATCCAACTGCCGATa   <  1:20265/140‑1 (MQ=255)
                                                                                                                            cGCCTTGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATCCAACTGCCGATaa  >  1:448846/1‑140 (MQ=255)
                                                                                                                                 |                                                                                                                                      
ACGAAGGGGGACCATTGGTGAATGATCTGTTTTGCACAATACCGGGCCAGCATCACGCTGGCCCGCGGTTTCAGGGAATGAGCAACACTTTGCCCGGCATAGCATTACGAGCGATGTCACGCACCGCCTGGGCGAAGCTTTCAAAGCTTCCACGGTGAGCGATTAATGGCTCCAGGCTTAACTTACGTTCTGTCAGCAACCGGCTCGCCGTTTCCCACTCCTGCCCCGGCCAAGGGCTGGAGTAGTTCATCCAACTGCCGATAA  >  NC_000913/2171763‑2172026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑