Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,216,697	C→A	A407E (GCA→GAA)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,216,697	0	C	A	100.0%	167.9 / NA	50	A407E (GCA→GAA)	aceB	malate synthase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/26);  total (24/26)

GATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTG  >  NC_000913/4216561‑4216831                                                                                                                                         |                                                                                                                                       gATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAAcc                                                                                                                                     <  1:842354/140‑1 (MQ=255)   tGGCACATGGATCGCTCACCCAGGCCCTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCtt                                                                                                                                   >  1:365086/1‑140 (MQ=255)    ggCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTtg                                                                                                                                  <  2:597853/140‑1 (MQ=255)     gCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTtgt                                                                                                                                 >  2:420227/1‑140 (MQ=255)      cacaTGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTtgtg                                                                                                                                <  2:299671/140‑1 (MQ=255)      cacaTGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTtgtg                                                                                                                                <  1:425937/140‑1 (MQ=255)           ggATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGt                                                                                                                           >  2:346536/1‑140 (MQ=255)           ggATCGCTCACCCAGGCCCTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGt                                                                                                                           <  2:365086/140‑1 (MQ=255)               cGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTTTCGGCTCCCGTAAAAATCAGGTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAAc                                                                                                                       >  2:640321/1‑140 (MQ=255)               cGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAAc                                                                                                                       >  2:854060/1‑140 (MQ=255)                 cTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGc                                                                                                                     >  2:505603/1‑140 (MQ=255)                    aCCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCAcc                                                                                                                  >  2:278831/1‑140 (MQ=255)                    aCCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACG‑GCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCg                                                                                                                 >  1:60952/1‑140 (MQ=255)                      ccAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCga                                                                                                                <  2:241485/140‑1 (MQ=255)                      ccAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCga                                                                                                                <  2:664557/140‑1 (MQ=255)                          gCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCgaagaa                                                                                                            >  2:856043/1‑140 (MQ=255)                                gggACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATg                                                                                                      >  2:666840/2‑140 (MQ=255)                                cGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATg                                                                                                      <  2:725973/140‑1 (MQ=255)                                 ggACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATgc                                                                                                     <  2:4441/140‑1 (MQ=255)                                     acGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATgcgcgc                                                                                                 <  1:437019/140‑1 (MQ=255)                                             ggCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACG‑GCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTcg                                                                                        <  2:60952/140‑1 (MQ=255)                                              gCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTcg                                                                                        <  2:312816/140‑1 (MQ=255)                                              gCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTcg                                                                                        <  1:346536/140‑1 (MQ=255)                                               cGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTcgc                                                                                       <  1:854060/140‑1 (MQ=255)                                                   aTTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTgg                                                                                   <  2:120957/140‑1 (MQ=255)                                                   aTTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTgg                                                                                   <  1:59953/140‑1 (MQ=255)                                                       aaCGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCtgt                                                                               >  2:373991/1‑140 (MQ=255)                                                        aCGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCtgtg                                                                              <  1:78422/140‑1 (MQ=255)                                                        aCGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCtgtg                                                                              <  1:535209/140‑1 (MQ=255)                                                              ttCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTAc                                                                        >  1:59120/1‑140 (MQ=255)                                                              ttCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTAc                                                                        >  1:250409/1‑140 (MQ=255)                                                              ttCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTAc                                                                        >  1:72316/1‑140 (MQ=255)                                                              ttCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTAc                                                                        >  2:327857/1‑140 (MQ=255)                                                                   ggCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGa                                                                   >  2:649266/1‑140 (MQ=255)                                                                       cccGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCg                                                               <  1:373991/140‑1 (MQ=255)                                                                         cGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTg                                                             >  2:563017/1‑140 (MQ=255)                                                                                aTCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATctct                                                      <  2:745701/140‑1 (MQ=255)                                                                                             gATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCtgtg                                         >  2:198724/1‑140 (MQ=255)                                                                                               tGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCtgtgtg                                       >  2:609987/1‑140 (MQ=255)                                                                                                 cgcgAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGcc                                     <  1:327857/140‑1 (MQ=255)                                                                                                   cgAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGa                                   >  1:168299/1‑140 (MQ=255)                                                                                                       cAAGACGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTa                               <  1:609987/140‑1 (MQ=255)                                                                                                           ccgcgCCGATTACTGCTGATCAGCTGCTGCAACCTTGTGATGGTGACCGCCCCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGc                           <  1:640321/139‑1 (MQ=255)                                                                                                           aCGCGCCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGc                           <  1:112274/140‑1 (MQ=255)                                                                                                               gcCGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGa                       >  2:25018/1‑140 (MQ=255)                                                                                                                ccGATTACTGCCGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGAt                      >  2:880557/1‑140 (MQ=255)                                                                                                                          ccGATCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATgcg            >  1:244158/1‑140 (MQ=255)                                                                                                                              tCAGCTGCTGGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGACGATTTATGGCCTGATGGAAGATGCGGCGa        <  2:543371/140‑1 (MQ=255)                                                                                                                              gacagtgctgGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGa        <  2:937873/135‑1 (MQ=255)                                                                                                                                    gctgGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTg  >  2:461406/1‑140 (MQ=255)                                                                                                                                    gctgGAACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTg  >  1:47703/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       GATGGCACATGGATCGCTCACCCAGGCCTTGCGGACACGGCAATGGCGGTATTCAACGACATTCTCGGCTCCCGTAAAAATCAGCTTGAAGTGATGCGCGAACAAGACGCGCCGATTACTGCCGATCAGCTGCTGGCACCTTGTGATGGTGAACGCACCGAAGAAGGTATGCGCGCCAACATTCGCGTGGCTGTGCAGTACATCGAAGCGTGGATCTCTGGCAACGGCTGTGTGCCGATTTATGGCCTGATGGAAGATGCGGCGACGGCTG  >  NC_000913/4216561‑4216831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑