Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,034,143	C→A	S297Y (TCT→TAT)	hyaB →	hydrogenase 1 large subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,034,143	0	C	A	100.0%	171.6 / NA	51	S297Y (TCT→TAT)	hyaB	hydrogenase 1 large subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (28/23);  total (28/23)

TCAATATGGAACGCCTGAACCTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTCTGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGAT  >  NC_000913/1034008‑1034276                                                                                                                                        |                                                                                                                                      tCAATATGGAACGCCTGAACCTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGAt                                                                                                                                   >  2:34321/1‑140 (MQ=255)      atGGAACGCCTGAACCTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATg                                                                                                                              >  1:446608/1‑140 (MQ=255)            cGCCTGAACCTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTtct                                                                                                                        >  2:787252/1‑140 (MQ=255)            cGCCTGAACCTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTtct                                                                                                                        <  2:163158/140‑1 (MQ=255)               cTGAACCTGGTGCAGTCAATTATCACCCGCACGGCGTACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAg                                                                                                                     >  2:273988/1‑140 (MQ=255)               cTGAACCTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAg                                                                                                                     >  1:216687/1‑140 (MQ=255)                  aaCCTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTa                                                                                                                  <  2:620692/140‑1 (MQ=255)                     cTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACgg                                                                                                               <  2:446608/140‑1 (MQ=255)                       ggTGCAGGCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGgcg                                                                                                             <  1:437639/140‑1 (MQ=255)                        gTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACggtg                                                                                                             >  2:492558/1‑137 (MQ=255)                        gTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACggtg                                                                                                             <  1:492558/139‑3 (MQ=255)                        gTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGgcgc                                                                                                            <  1:595624/140‑1 (MQ=255)                          gCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCAt                                                                                                          >  1:234427/1‑140 (MQ=255)                             gTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTcc                                                                                                       >  2:859484/1‑140 (MQ=255)                                        cccGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGcc                                                                                            >  1:422911/1‑140 (MQ=255)                                            cACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACg                                                                                        >  2:783502/1‑140 (MQ=255)                                               ggcggACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACt                                                                                     <  1:859484/140‑1 (MQ=255)                                                        cATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCgaga                                                                            <  2:234427/140‑1 (MQ=255)                                                         aTTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGaa                                                                           <  2:22656/140‑1 (MQ=255)                                                           tAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGaaaa                                                                         >  1:717239/1‑140 (MQ=255)                                                                acGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTctg                                                                    >  1:659515/1‑140 (MQ=255)                                                                acGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTctg                                                                    >  2:546908/1‑140 (MQ=255)                                                                    gatgatCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGa                                                                >  2:682388/1‑140 (MQ=255)                                                                         tCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCt                                                           >  1:773179/1‑140 (MQ=255)                                                                           cccGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTgg                                                         >  2:236836/1‑140 (MQ=255)                                                                           cccGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTgg                                                         >  2:220418/1‑140 (MQ=255)                                                                             cGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTggcg                                                       >  1:610467/1‑140 (MQ=255)                                                                             cGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTggcg                                                       >  1:482219/1‑140 (MQ=255)                                                                                cGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGgcggcg                                                    <  1:236836/140‑1 (MQ=255)                                                                                  ccTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGcgcg                                                  <  2:216687/140‑1 (MQ=255)                                                                                  ccTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGcgcg                                                  <  2:659515/140‑1 (MQ=255)                                                                                  ccTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGcgcg                                                  >  1:572173/1‑140 (MQ=255)                                                                                             ggTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACgg                                       >  2:525162/1‑140 (MQ=255)                                                                                              gTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGc                                      <  1:4199/140‑1 (MQ=255)                                                                                              gTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGc                                      <  1:787252/140‑1 (MQ=255)                                                                                              gTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGc                                      <  2:773179/140‑1 (MQ=255)                                                                                                cAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGa                                    <  2:572173/140‑1 (MQ=255)                                                                                                 aGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGAc                                   <  1:525162/140‑1 (MQ=255)                                                                                                   ttCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACtt                                 <  1:546975/140‑1 (MQ=255)                                                                                                       acaaACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTcaac                             <  2:39792/140‑1 (MQ=255)                                                                                                       acaaACCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTcaac                             <  2:610467/140‑1 (MQ=255)                                                                                                          aaCCGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAAt                          <  2:482219/140‑1 (MQ=255)                                                                                                             cGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAAtgtg                       >  2:501186/1‑140 (MQ=255)                                                                                                             cGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAAtgtg                       >  1:742137/1‑140 (MQ=255)                                                                                                             cGTGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAAtgtg                       >  2:733684/1‑140 (MQ=255)                                                                                                               tGGAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGtgct                     >  1:201105/1‑140 (MQ=255)                                                                                                                 gAGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGtgctgc                   <  1:783502/140‑1 (MQ=255)                                                                                                                  aGCGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGcc                  >  1:772894/1‑140 (MQ=255)                                                                                                                    cGAAATCGGCACTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAg                >  2:300736/1‑140 (MQ=255)                                                                                                                              aCTGGTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGt      <  1:511437/140‑1 (MQ=255)                                                                                                                                  gTCTTTATGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGAt  >  2:780688/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      TCAATATGGAACGCCTGAACCTGGTGCAGTCAATTATCACCCGCACGGCGGACTTCATTAACAACGTGATGATCCCCGACGCCTTAGCCATCGGTCAGTTCAACAAACCGTGGAGCGAAATCGGCACTGGTCTTTCTGATAAATGCGTTCTCAGCTACGGCGCATTCCCGGATATTGCCAACGACTTTGGCGAGAAAAGTCTGCTGATGCCTGGCGGCGCGGTGATTAACGGCGACTTCAACAATGTGCTGCCAGTGGATTTGGTTGAT  >  NC_000913/1034008‑1034276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑