Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,110,367	C→A	noncoding (2/76 nt)	pheV →	tRNA‑Phe

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,110,367	0	C	A	100.0%	122.5 / NA	36	noncoding (2/76 nt)	pheV	tRNA‑Phe
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/14);  total (22/14)

TCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAAC  >  NC_000913/3110231‑3110498                                                                                                                                         |                                                                                                                                    tCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCg                                                                                                                                  <  2:871304/140‑1 (MQ=255)         cTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTc                                                                                                                          <  1:933745/140‑1 (MQ=255)               cGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCgg                                                                                                                    <  2:271994/140‑1 (MQ=255)               cGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCgg                                                                                                                    <  2:169875/140‑1 (MQ=255)               cGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCgg                                                                                                                    <  2:142286/140‑1 (MQ=255)                 ccTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTa                                                                                                                  >  1:62973/1‑140 (MQ=255)                 ccTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTa                                                                                                                  >  1:897839/1‑140 (MQ=255)                     gTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGc                                                                                                              <  1:644720/140‑1 (MQ=255)                     gTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGc                                                                                                              <  1:920336/140‑1 (MQ=255)                      tttGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCa                                                                                                             <  2:62973/140‑1 (MQ=255)                                   gCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAAt                                                                                                >  1:78104/1‑140 (MQ=255)                                    cGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCa                                                                                                             >  2:757068/1‑126 (MQ=255)                                    cGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCa                                                                                                             <  1:757068/126‑1 (MQ=255)                                            atCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTc                                                                                       >  2:607594/1‑140 (MQ=255)                                              cGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCt                                                                                     <  1:340000/140‑1 (MQ=255)                                              cGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGGTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCt                                                                                     <  2:897839/140‑1 (MQ=255)                                                               aTTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTc                                                                    >  2:266896/1‑140 (MQ=255)                                                                ttGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTcc                                                                   >  1:899862/1‑140 (MQ=255)                                                                  gtgCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCgg                                                                 >  1:375728/1‑140 (MQ=255)                                                                   tgCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCggg                                                                <  2:165152/140‑1 (MQ=255)                                                                   tgCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCggg                                                                >  2:169737/1‑140 (MQ=255)                                                                   tgCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCggg                                                                >  2:510553/1‑140 (MQ=255)                                                                    gCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGc                                                               <  1:169737/140‑1 (MQ=255)                                                                    gCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGc                                                               <  2:767682/140‑1 (MQ=255)                                                                     cAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGca                                                              >  1:830731/1‑140 (MQ=255)                                                                          tGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCg                                                                  >  1:470740/1‑131 (MQ=255)                                                                          tGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCg                                                                  <  2:470740/131‑1 (MQ=255)                                                                          tGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACt                                                         >  2:163399/1‑140 (MQ=255)                                                                             aGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAAt                                                      >  2:121842/1‑140 (MQ=255)                                                                                    tGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAg                                               >  1:876112/1‑140 (MQ=255)                                                                                           aTTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGc                                        >  2:338653/1‑140 (MQ=255)                                                                                                              gCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGc                     >  1:658323/1‑140 (MQ=255)                                                                                                              gCGAATCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGc                     >  2:808689/1‑140 (MQ=255)                                                                                                                   tCAGGTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTg                >  1:424988/1‑140 (MQ=255)                                                                                                                       gTTTAATGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTCTTGCTTTTGGATc            <  2:424988/140‑1 (MQ=255)                                                                                                                             tGCGCCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACaa      >  2:151017/1‑140 (MQ=255)                                                                                                                                gcCCCGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATaa   >  1:292316/1‑140 (MQ=255)                                                                                                                                 ccccGTTGACCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAAc  >  1:357529/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                    TCCGCCGCCTGGACCGCCTGGTTTGCCTGACAATGCGTGCAATATCGGCAAAGTGATGATAGATTGTGCAGTCTGCAGTAAATTGAAGAAATTTGATTGACGAGACGAGGCGAATCAGGTTTAATGCGCCCCGTTGCCCGGATAGCTCAGTCGGTAGAGCAGGGGATTGAAAATCCCCGTGTCCTTGGTTCGATTCCGAGTCCGGGCACCACTAATTCTTAAGAACCCGCCCACAAGGCGGGTTTTTGCTTTTGGATCTGACAATAAC  >  NC_000913/3110231‑3110498

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑