Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,849,360	G→T	L164M (CTG→ATG)	hycB ←	formate hydrogenlyase subunit HycB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,849,360	0	G	T	100.0%	142.2 / NA	41	L164M (CTG→ATG)	hycB	formate hydrogenlyase subunit HycB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/21);  total (20/21)

CAGGGAAATTGCGCTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCAGGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGC  >  NC_000913/2849224‑2849495                                                                                                                                         |                                                                                                                                        cAGGGAAATTGCGCTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGc                                                                                                                                      >  1:721647/1‑140 (MQ=255)   gggAAATTGCGCTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCtt                                                                                                                                    <  2:721647/140‑1 (MQ=255)      aaaTTGCGCTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAg                                                                                                                                 >  2:266274/1‑140 (MQ=255)       aaTTGCGCTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGt                                                                                                                                <  2:365765/140‑1 (MQ=255)         ttGCGCTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCg                                                                                                                              >  1:908756/1‑140 (MQ=255)             gcTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGca                                                                                                                          <  1:231012/140‑1 (MQ=255)             gcTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGca                                                                                                                          <  1:695659/140‑1 (MQ=255)              cTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGcac                                                                                                                         >  2:95342/1‑140 (MQ=255)                       ccTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCcgcacg                                                                                                                <  1:95342/140‑1 (MQ=255)                           tcCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAgg                                                                                                            >  1:184730/1‑140 (MQ=255)                           tcCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAgg                                                                                                            >  2:452236/1‑140 (MQ=255)                           tcCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAgg                                                                                                            >  1:898365/1‑140 (MQ=255)                           tcCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAgg                                                                                                            >  1:46248/1‑140 (MQ=255)                            ccACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGc                                                                                                           <  2:908756/140‑1 (MQ=255)                                  tGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGAcc                                                                                                     <  1:79383/140‑1 (MQ=255)                                     gCCTGCTGAAACAAGGTGAGCTCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTg                                                                                                  <  2:635332/140‑1 (MQ=255)                                          ctgAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTtcat                                                                                             <  2:898365/140‑1 (MQ=255)                                          ctgAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTtcat                                                                                             <  1:266274/140‑1 (MQ=255)                                          ctgAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTtcat                                                                                             <  1:552641/140‑1 (MQ=255)                                             aaaCAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCaa                                                                                          <  2:184730/140‑1 (MQ=255)                                               aCAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAg                                                                                        <  2:377696/140‑1 (MQ=255)                                                    gTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACa                                                                                   <  1:690023/140‑1 (MQ=255)                                                       agaTCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAg                                                                                >  2:905283/1‑140 (MQ=255)                                                        gaTCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAgg                                                                               <  2:156200/140‑1 (MQ=255)                                                         aTCGCCAAAGTCCGTGTTAAAGGGCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCTGGCACATCCGCACGCAGGCCGGCCCTTGTTCATCAAAGCTACAAAGGt                                                                              >  2:710777/1‑140 (MQ=255)                                                                                 cAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGaa                                                      >  2:791416/1‑140 (MQ=255)                                                                                  aGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAAt                                                     >  2:943646/1‑140 (MQ=255)                                                                                  aGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAAt                                                     >  2:621150/1‑140 (MQ=255)                                                                                     tCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATAcc                                                  <  2:168592/140‑1 (MQ=255)                                                                                          cTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCa                                             <  2:632001/140‑1 (MQ=255)                                                                                              cGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCa                                         <  1:717535/140‑1 (MQ=255)                                                                                                      gACGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCa                                 <  1:943646/140‑1 (MQ=255)                                                                                                       aCGCGGGCGATATCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCaa                                >  1:594427/1‑140 (MQ=255)                                                                                                                 tatCGGTGTTATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGaca                      >  1:191481/1‑140 (MQ=255)                                                                                                                         ttATCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCgg              >  1:200271/1‑140 (MQ=255)                                                                                                                           aTCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAg            >  1:175420/1‑140 (MQ=255)                                                                                                                           aTCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAg            >  2:723123/1‑140 (MQ=255)                                                                                                                            tCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGc           >  2:871717/1‑140 (MQ=255)                                                                                                                            tCCACCAGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGc           >  1:683027/1‑140 (MQ=255)                                                                                                                                 caGATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCg      >  1:687578/1‑140 (MQ=255)                                                                                                                                   gATGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGt    <  1:452236/140‑1 (MQ=255)                                                                                                                                     tGCATGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGc  <  2:594427/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        CAGGGAAATTGCGCTCATTTAGCCTCTCCACTTTGAGCCTGCTGAAACAAGGTGAGATCGCCAAAGTCCGTGTTAAAGGTCAGCTCACGCTTACGTTTGCTGACGCGGGCGATATCGGTGTTATCCACCAGATGCAGGGCTTTAGTCGGGCACATCCGCACGCAGGCCGGACCTTGTTCATCAAAGCTACAAAGGTCACATTTGACGGCGATCGCGCGAATACCTGGCACCCAGTCAAGCAATGTGCTGACACGCGCCGGAGCAGGCGGTGC  >  NC_000913/2849224‑2849495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑