Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,005,686	A→T	I34N (ATC→AAC)	ygeV ←	putative sigma(54)‑dependent transcriptional regulator YgeV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,005,686	0	A	T	100.0%	130.1 / NA	38	I34N (ATC→AAC)	ygeV	putative sigma(54)‑dependent transcriptional regulator YgeV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (15/23);  total (15/23)

TGTCACAACTTTTTCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGATCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGCTTTACCTTCCAGACTTACTTAAAAGTCGA  >  NC_000913/3005559‑3005818                                                                                                                                |                                                                                                                                     tgtCACAACTTTTTCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCaa                                                                                                                          >  2:283703/1‑140 (MQ=255) tgtCACAACTTTTTCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCaa                                                                                                                          >  1:272207/1‑140 (MQ=255)  gtCACAACTTTTTCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAg                                                                                                                         <  1:16042/140‑1 (MQ=255)  gtCACAACTTTTTCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAg                                                                                                                         <  2:158161/140‑1 (MQ=255)      caACTTTTTCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGc                                                                                                                     >  2:461124/1‑140 (MQ=255)           ttttCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCa                                                                                                                           <  1:100005/129‑1 (MQ=255)           ttttCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCa                                                                                                                           >  2:100005/1‑129 (MQ=255)           ttttCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAAc                                                                                                                >  2:66116/1‑140 (MQ=255)                 gTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGc                                                                                                          <  1:11694/140‑1 (MQ=255)                    ttCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAg                                                                                                       >  2:311384/1‑140 (MQ=255)                    ttCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAg                                                                                                       >  1:77842/1‑140 (MQ=255)                         ttCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTc                                                                                                  <  1:413462/140‑1 (MQ=255)                                  gTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAAc                                                                                         <  1:150481/140‑1 (MQ=255)                                           cAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTg                                                                                >  2:95572/1‑140 (MQ=255)                                           cAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTg                                                                                <  1:122642/140‑1 (MQ=255)                                              gCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCa                                                                                             >  1:265830/1‑124 (MQ=255)                                              gCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCa                                                                                             <  2:265830/124‑1 (MQ=255)                                                      tGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAAtt                                                                     <  2:77842/140‑1 (MQ=255)                                                          gCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCa                                                                 <  1:270653/140‑1 (MQ=255)                                                          gCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCa                                                                 <  2:200278/140‑1 (MQ=255)                                                             cAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCa                                                              >  1:333713/1‑140 (MQ=255)                                                                      aCCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATactgact                                                     <  1:95572/140‑1 (MQ=255)                                                                      aCCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATactgact                                                     >  1:39858/1‑140 (MQ=255)                                                                         aaGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCg                                                  <  2:39858/140‑1 (MQ=255)                                                                           gAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCgta                                                <  1:288613/140‑1 (MQ=255)                                                                                 tCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCa                                          <  1:461124/140‑1 (MQ=255)                                                                                 tCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCa                                          <  2:333713/140‑1 (MQ=255)                                                                                    cATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGc                                       >  1:434554/1‑140 (MQ=255)                                                                                          cgcCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGCAGCAAGCTCCATa                                 >  2:62664/1‑140 (MQ=255)                                                                                            cccGTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGc                               >  1:236436/1‑140 (MQ=255)                                                                                               gTTCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGCttt                            <  2:465505/140‑1 (MQ=255)                                                                                                ttCCGGCAACGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGCAGCAAGCTCCATAGCTTTa                           <  1:62664/140‑1 (MQ=255)                                                                                                        aCGCGACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATGGCTTTACCTTCCAg                   <  1:183105/140‑1 (MQ=255)                                                                                                          gcgACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGCTTTACCTTCCAGac                 <  2:376795/140‑1 (MQ=255)                                                                                                           cgACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGCTTTACCTTCCAGact                <  2:355395/140‑1 (MQ=255)                                                                                                           cgACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGCTTTACCTTCCAGact                <  1:66116/140‑1 (MQ=255)                                                                                                            gACACAAGTTTTCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGCTTTACCTTCCAGactt               <  2:117392/140‑1 (MQ=255)                                                                                                                     tttCATCAACGTTCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGCTTTACCTTCCAGACTTACTTAAAAg      >  2:27113/1‑140 (MQ=255)                                                                                                                         atcatcGTTCTCAACCTGAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAAAGCTGAATTGTCGGTTGAATTTGCATCAATACTTACTGCGTAGTAGCAAGCTCCATAGCTTCACCTTCCAGACTTACTTAATAGTCGa  <  2:378271/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                     TGTCACAACTTTTTCAGTTTTCGTTTCCAGGACGTGGCGGAGCAGGCGTGAGTTGCCGCTCAACTGGCGACCAAGAAACTTCCCATACGCGCCCGTTCCGGCAACGCGACACAAGTTTTCATCAACGATCTCAACCTCAAGCTGCAAAACGCTGGCAAGCATTCTGGCAAAACGCTGAATTGTCGGTTGAATTTGCATCAATACTGACTGCGTAGTAGCAAGCTCCATAGCTTTACCTTCCAGACTTACTTAAAAGTCGA  >  NC_000913/3005559‑3005818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑