Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,627,014	G→T	S842Y (TCC→TAC)	rbbA ←	ribosome‑associated ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,627,014	0	G	T	100.0%	92.3 / NA	27	S842Y (TCC→TAC)	rbbA	ribosome‑associated ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/16);  total (11/16)

CCACAAATCAGTCAGATCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGGAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAATGATGATATACAGCAGCGCCGCCAGGGTGAGCG  >  NC_000913/3626884‑3627149                                                                                                                                   |                                                                                                                                        ccACAAATCAGTCAGATCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACtgtg                                                                                                                                <  1:396418/140‑1 (MQ=255)               gATCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAAc                                                                                                                  >  1:24965/1‑140 (MQ=255)                   cAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGa                                                                                                              >  2:187777/1‑140 (MQ=255)                      cgcTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATaa                                                                                                           >  1:429540/1‑140 (MQ=255)                          ttCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGc                                                                                                       >  1:148406/1‑140 (MQ=255)                                         cgGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATggc                                                                                        <  1:187777/140‑1 (MQ=255)                                                           tGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCAt                                                                      <  2:148406/140‑1 (MQ=255)                                                             aCTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATaa                                                                    >  1:155607/1‑140 (MQ=255)                                                             aCTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATaa                                                                    <  1:401191/140‑1 (MQ=255)                                                                    gggTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGga                                                             <  2:369628/140‑1 (MQ=255)                                                                     ggTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGgag                                                            <  2:155607/140‑1 (MQ=255)                                                                           ccTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATcagc                                                      <  1:49210/140‑1 (MQ=255)                                                                           ccTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATcagc                                                      >  1:455601/1‑140 (MQ=255)                                                                                ccGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGACCAGCAGccc                                                 >  2:7298/1‑140 (MQ=255)                                                                                              aGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACTTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCaa                                   <  2:446057/140‑1 (MQ=255)                                                                                                gCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAatg                                 >  1:399020/1‑140 (MQ=255)                                                                                                gCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAatg                                 <  1:217951/140‑1 (MQ=255)                                                                                                    ttCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAAtgatga                             <  2:24965/140‑1 (MQ=255)                                                                                                     tCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCaa                                   >  2:209779/1‑133 (MQ=255)                                                                                                     tCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCaa                                   <  1:209779/133‑1 (MQ=255)                                                                                                     tCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAatga                                <  1:383755/136‑1 (MQ=255)                                                                                                     tCCAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAatga                                >  2:383755/1‑136 (MQ=255)                                                                                                      ccAGCGAAGCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAATGATGata                           <  2:432875/140‑1 (MQ=255)                                                                                                              gCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAATGATGATATAcagcag                   <  2:301154/140‑1 (MQ=255)                                                                                                              gCTACCGGATCGATCATCCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAATGATGATATAcagcag                   <  2:455601/140‑1 (MQ=255)                                                                                                                          atcatcCCGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAATGATGATATACAGCAGCGCCGCCAGGGt       <  1:55564/140‑1 (MQ=255)                                                                                                                               cccGTAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAATGATGATATACAGCAGCGCCGCCAGGGTGAGCg  >  1:320870/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                        CCACAAATCAGTCAGATCCAGCGCTTTCGAGAACGTCCCGCGGGCGATAGTCAGAAAATGACTGGTCGGGTAAACCTCGCCGATCCAACGTCCAGGCCCTTCCAGCGAAGCTACCGGATCGATCATCCCGGAAAACTGTGTCGCCGGGATCAACGTGATAATCGCCGTTCCGAAAATGGCGGCAATCTGGCTTTTCATAAAGGTGGAGATCAGCAGCCCCATTCCGGTGGCAATGATGATATACAGCAGCGCCGCCAGGGTGAGCG  >  NC_000913/3626884‑3627149

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑