| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,982,842 | G→T | intergenic (+31/‑116) | proM → / → aslB | tRNA‑Pro/putative anaerobic sulfatase maturation enzyme AslB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,982,842 | 0 | G | T | 81.2% | 41.3 / 4.4 | 16 | intergenic (+31/‑116) | proM/aslB | tRNA‑Pro/putative anaerobic sulfatase maturation enzyme AslB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base T (2/11); minor base . (2/1); total (4/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.86e-01 | |||||||||||
TTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAAC > NC_000913/3982706‑3982967 | ttGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGtttttttttt < 2:50194/140‑1 (MQ=255) aTTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCAttt < 1:266944/140‑1 (MQ=255) cAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTT‑TTTTCTGTGCATTTCGTCAccctcc < 2:389935/140‑1 (MQ=255) ggAGTAGCGCAACTTGGGAGCGCAAATGGTTTGGGACCAGTGGGTCGGGGGTTCCAATCCTCTCTCGCCGGCCAATTTTGAGCCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATa < 2:394953/139‑1 (MQ=255) aGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTTTCTGCATTTTGTCACCCTCCCTTCCCCATAAACGc > 2:428622/1‑140 (MQ=38) gcgcAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGcc < 2:341572/140‑1 (MQ=255) gcgcAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGcc < 1:428622/140‑1 (MQ=255) cgcAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTT‑TTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTg > 1:126355/1‑140 (MQ=255) aaCTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGcc < 2:58491/140‑1 (MQ=255) aCTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCACAATAAACGCCCGTAATAACTCATTGccc < 2:70196/140‑1 (MQ=255) tGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCa < 2:80267/140‑1 (MQ=255) tGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTa < 2:239834/140‑1 (MQ=255) ctctcGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTTCCCCTTAACGTATTGAAGGATGa > 2:190795/1‑140 (MQ=255) gACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGc < 1:98613/140‑1 (MQ=255) gAACCCCGCTTCGGCGGGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGAc < 2:252770/140‑1 (MQ=255) cGCTTCGGCGGGGTTTTTTTTTTTCTGTGGATTTCTGCACCCCCCCTTCGCAATAAACGGCCGTAATAACTCCTTGCCCCACGGTTTTATTTCCCCCTTAAACGATTGAAGGATGACTTCAGGCAAGGAGCGACCaggtt > 2:40677/1‑136 (MQ=255) cGCTTCGGCGGGGTTTTTT‑TTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCAtgctg > 2:445304/1‑140 (MQ=255) cggcggGGTTTTTTTTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAAc < 1:453777/140‑1 (MQ=255) | TTGAACTAAAAATTCAAAAAGCAGTATTTCGGCGAGTAGCGCAGCTTGGTAGCGCAACTGGTTTGGGACCAGTGGGTCGGAGGTTCGAATCCTCTCTCGCCGACCAATTTTGAACCCCGCTTCGGCGGGGTTTTTTGTTTTCTGTGCATTTCGTCACCCTCCCTTCGCAATAAACGCCCGTAATAACTCATTGCCCCACGGTATGATTTCGCCCTTAACGTATTGAAGGATGACTTCAGGCAAGGAGCGACCATGCTGCAAC > NC_000913/3982706‑3982967 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |