Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,378,205	G→T	A247D (GCC→GAC)	ampC ←	beta‑lactamase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,378,205	0	G	T	100.0%	79.2 / NA	24	A247D (GCC→GAC)	ampC	beta‑lactamase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/14);  total (10/14)

CAGCCCAGGCCCTGATACATATCGCCGGTTTGCCAGTAGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTA  >  NC_000913/4378085‑4378339                                                                                                                         |                                                                                                                                       cagcccagGCCCTGATACATATCGCCGGTTTGCCAGTAGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGa                                                                                                                     <  1:391121/140‑1 (MQ=255)         gCCCTGATACATATCGCCGGTTTGCCAGTAGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCacac                                                                                                             <  1:380166/140‑1 (MQ=255)                   atatCGCCGGTTTGCCAGTAGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTc                                                                                                   >  1:258206/1‑140 (MQ=255)                     atCGCCGGTTTGCCAGTAGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAg                                                                                                 >  1:396217/1‑140 (MQ=255)                         ccGGTTTGCCAGTAGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCGGCATc                                                                                             >  1:289730/1‑140 (MQ=255)                                 ccAGTAGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGccc                                                                                     <  1:71765/140‑1 (MQ=255)                                      aGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGc                                                                                <  2:387846/140‑1 (MQ=255)                                                            cccTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCtt                                                          <  2:189663/140‑1 (MQ=255)                                                                tgttgAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCCTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTcgcg                                                      >  2:27128/1‑140 (MQ=255)                                                                       gCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATccc                                               <  1:249246/140‑1 (MQ=255)                                                                           tttCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAgg                                           <  2:54490/140‑1 (MQ=255)                                                                           tttCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAgg                                           <  2:95885/140‑1 (MQ=255)                                                                                               gTTTTAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTgcg                       <  2:316173/140‑1 (MQ=255)                                                                                                ttttAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTgcgg                      >  1:30627/1‑140 (MQ=255)                                                                                                  ttAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTgcgggc                    <  2:396217/140‑1 (MQ=255)                                                                                                   tAAATTGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTgcgggcg                   <  2:30627/140‑1 (MQ=255)                                                                                                     aaTTGCTTTGCACCTAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGt                 <  2:374953/140‑1 (MQ=255)                                                                                                        tGCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACa              >  1:376615/1‑140 (MQ=255)                                                                                                         gCTTTGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACAt             >  1:377917/1‑140 (MQ=255)                                                                                                            ttGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTaa          <  2:264150/140‑1 (MQ=255)                                                                                                             tGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAAt         >  1:390328/1‑140 (MQ=255)                                                                                                             tGCACCCAGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAAt         <  2:390328/140‑1 (MQ=255)                                                                                                                    aGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTa  >  1:318822/1‑140 (MQ=255)                                                                                                                    aGCGGTCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTa  >  2:61141/1‑140 (MQ=255)                                                                                                                         |                                                                                                                                       CAGCCCAGGCCCTGATACATATCGCCGGTTTGCCAGTAGCGAGATTGTGCCAGTTGTATCCCTTGTTGAAGCGTTTTCTCATTGATATCAAGGGGTTTTAAATTGCTTTGCACCCAGCGGGCCATATCTTCAATGGTCGACTTCACACCATAAGCTTCAGCATCTAACGCCCCAGGCGAAACATGCACTGCCTTACCTTCGCGATATCCCCAGGCGTAATTCTTTTCTTCTGCGGGCGGTACATTAATCCACGTA  >  NC_000913/4378085‑4378339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑