Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,160,915	G→A	P213S (CCA→TCA)	fhuE ←	ferric coprogen/ferric rhodotorulic acid outer membrane transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,160,915	0	G	A	100.0%	59.8 / NA	19	P213S (CCA→TCA)	fhuE	ferric coprogen/ferric rhodotorulic acid outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

GATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCA  >  NC_000913/1160786‑1161027                                                                                                                                  |                                                                                                                 gATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGG‑AGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAAt                                                                                                       <  2:103589/140‑1 (MQ=255)                cATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGt                                                                                         >  2:384469/1‑140 (MQ=255)                      cAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTg                                                                                   <  1:319408/140‑1 (MQ=255)                          gCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTcc                                                                               <  1:411258/140‑1 (MQ=255)                                   gaagGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGt                                                                      >  2:312284/1‑140 (MQ=255)                                     agGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTAt                                                                    >  1:252336/1‑140 (MQ=255)                                           tttCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgcc                                                              >  1:108616/1‑140 (MQ=255)                                            ttCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgccg                                                             >  1:12796/1‑140 (MQ=255)                                            ttCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTccgccg                                                             >  2:126512/1‑140 (MQ=255)                                                 tgttgtAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACa                                                        <  2:185634/140‑1 (MQ=255)                                                         cGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCttt                                                <  2:465090/140‑1 (MQ=255)                                                         cGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCttt                                                <  1:312284/140‑1 (MQ=255)                                                           gTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTaa                                              >  2:303162/1‑140 (MQ=255)                                                             ccAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAAt                                            >  1:439504/1‑140 (MQ=255)                                                                      aGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTg                                   >  1:351282/1‑140 (MQ=255)                                                                        tCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGt                                 <  1:303162/140‑1 (MQ=255)                                                                                     aGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGa                    <  2:327184/140‑1 (MQ=255)                                                                                        cgccgACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAAcc                 >  1:44151/1‑140 (MQ=255)                                                                                                       cgcgGATTTTACCGTCTTCGGTGAGTGAGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCa  <  1:328422/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                 GATCGCCTAAATCAGCATCGACAATGCCCGAGAAGAAGGTCTTTTCACTGTTGTAGCGGTCCAGCCATGAGTCGTTATTCTGGTAGCCGCCGACAATTCGCGCGCGGATTTTACCGTCTTCGGTGAGTGGGCTTTGTAAATCCGCCACATACCGTTCTTTGTTCCAGCTACCGTATTCCGCCGAGACATCGCCTTTAAATTCACGACTGGTCGCGTGTTTTCGAACCATATTAATTGCCGCA  >  NC_000913/1160786‑1161027

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑