Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,488,221	G→T	P316P (CCC→CCA)	yfdE ←	acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,488,221	0	G	T	100.0%	164.1 / NA	47	P316P (CCC→CCA)	yfdE	acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (21/26);  total (21/26)

ATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACGGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACC  >  NC_000913/2488084‑2488356                                                                                                                                          |                                                                                                                                        aTAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTGCCCGGCATCATTATTCCCCCGGCTTCAATCACCACAGTTCTCGGCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACCCTTAATAACGGCGCGACtgg                                                                                                                                       <  2:720588/140‑1 (MQ=255)     cATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTTCCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTaca                                                                                                                                   <  1:344548/140‑1 (MQ=255)     cATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTaca                                                                                                                                   <  2:740304/140‑1 (MQ=255)     cATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTaca                                                                                                                                   <  2:233065/140‑1 (MQ=255)      aTGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTacac                                                                                                                                  <  2:202947/140‑1 (MQ=255)        gCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCa                                                                                                                                <  1:267214/140‑1 (MQ=255)        gCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCa                                                                                                                                >  2:537594/1‑140 (MQ=255)          gggTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAAc                                                                                                                              >  1:281512/1‑140 (MQ=255)          gggTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAAc                                                                                                                              <  2:30604/140‑1 (MQ=255)             tCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAAc                                                                                                                              >  2:679058/1‑137 (MQ=255)             tCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAAc                                                                                                                              <  1:679058/137‑1 (MQ=255)              ccGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCa                                                                                                                          <  1:537594/140‑1 (MQ=255)                 cgcAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGt                                                                                                                       >  2:204539/1‑140 (MQ=255)                     gCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTc                                                                                                                   >  1:105120/1‑140 (MQ=255)                      ccGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCt                                                                                                                  >  1:40713/1‑140 (MQ=255)                                 tATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCaaa                                                                                                       <  1:592437/140‑1 (MQ=255)                                                          cccccGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGt                                                                              <  1:91508/140‑1 (MQ=255)                                                            cccGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTcc                                                                            <  1:445590/140‑1 (MQ=255)                                                            cccGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTcc                                                                            <  2:62974/140‑1 (MQ=255)                                                            cccGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGAGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTcc                                                                            <  1:204539/140‑1 (MQ=255)                                                             ccGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCg                                                                           >  1:239058/1‑140 (MQ=255)                                                             ccGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCg                                                                           >  1:217961/1‑140 (MQ=255)                                                              cGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGc                                                                          >  2:643542/1‑140 (MQ=255)                                                              cGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGc                                                                          <  1:587285/140‑1 (MQ=255)                                                               ggCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCt                                                                         <  2:21017/140‑1 (MQ=255)                                                                   tcaatcaaCATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAat                                                                     >  1:527502/1‑140 (MQ=255)                                                                      atcaaCATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAatata                                                                  <  2:412852/140‑1 (MQ=255)                                                                       tcaaCATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAatatat                                                                 <  1:312970/140‑1 (MQ=255)                                                                           cATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGtt                                                             >  2:205057/1‑140 (MQ=255)                                                                            atatTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGttt                                                            <  1:643542/140‑1 (MQ=255)                                                                                ttCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGa                                                        <  1:405863/140‑1 (MQ=255)                                                                                     gCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGc                                                   <  2:217961/140‑1 (MQ=255)                                                                                     gCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGc                                                   >  2:449807/1‑140 (MQ=255)                                                                                          aGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGt                                              >  1:510754/1‑140 (MQ=255)                                                                                          aGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGt                                              >  2:344388/1‑140 (MQ=255)                                                                                                    aaTTTAATGGCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTAcgcg                                    <  1:205057/140‑1 (MQ=255)                                                                                                             gCCTCAGCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATAtt                           <  1:344388/140‑1 (MQ=255)                                                                                                                   gCCACACTTAATAACGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTgctgct                     <  1:681387/140‑1 (MQ=255)                                                                                                                            aataaCGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCgggg            >  1:23049/1‑140 (MQ=255)                                                                                                                              taaCGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGAt          >  2:206253/1‑140 (MQ=255)                                                                                                                                aCGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCa        <  1:709459/140‑1 (MQ=255)                                                                                                                                 cGGCGCGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCAt       <  2:510754/140‑1 (MQ=255)                                                                                                                                   gcgcGACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTa     >  2:28853/1‑140 (MQ=255)                                                                                                                                    cgcgACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTaa    >  2:414856/1‑140 (MQ=255)                                                                                                                                    cgcgACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTaa    >  2:312337/1‑140 (MQ=255)                                                                                                                                    cgcgACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTaa    <  1:470204/140‑1 (MQ=255)                                                                                                                                    cgcgACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTaa    >  1:482717/1‑140 (MQ=255)                                                                                                                                      cgACTGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAAcc  >  1:619292/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        ATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAACATATTTCTCGCCTGAGTTTGTGGCAATTTAATGGCCTCAGCCACACTTAATAACGGCGCGACGGGTACACCAACTTCATGTATTCTGGCTAACCAAACTTCAGCTGCCTGCGTTTTTAACGTCCGCTCAATATATTGTTTAAGAATAGCCTGGTTTTGTACGCGTAAAATATTGCTGCTAAATCGGGGATCATTAACC  >  NC_000913/2488084‑2488356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑