Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	323,187	G→T	D284Y (GAT→TAT)	ykgF →	putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	323,187	0	G	T	100.0%	228.7 / NA	65	D284Y (GAT→TAT)	ykgF	putative amino acid dehydrogenase with NAD(P)‑binding domain and ferridoxin‑like domain
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (32/33);  total (32/33)

GATGGGAATGGAGCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGG  >  NC_000913/323051‑323323                                                                                                                                         |                                                                                                                                         gATGGGAATGGAGCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATg                                                                                                                                       <  2:507829/140‑1 (MQ=255) gATGGGAATGGAGCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATg                                                                                                                                       <  2:359403/140‑1 (MQ=255)  aTGGGAATGGAGCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATgg                                                                                                                                      >  2:101366/1‑140 (MQ=255)        aTGGAGCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGa                                                                                                                                <  2:593426/140‑1 (MQ=255)         tGGAGCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGaa                                                                                                                               <  1:552320/140‑1 (MQ=255)            aGCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAagag                                                                                                                            >  1:269151/1‑140 (MQ=255)             gCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGt                                                                                                                           <  1:173388/140‑1 (MQ=255)                 aTTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCa                                                                                                                       >  2:320319/1‑140 (MQ=255)                          aCGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTAt                                                                                                              >  2:233000/1‑140 (MQ=255)                             tttGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGt                                                                                                           <  1:490118/140‑1 (MQ=255)                             tttGCCAATGTAGATGTATTGATCACAATGCTGGCGGGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGt                                                                                                           <  1:779511/140‑1 (MQ=255)                                   gAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATaa                                                                                                     <  1:406171/140‑1 (MQ=255)                                      gTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACgg                                                                                                  >  1:110079/1‑140 (MQ=255)                                      gTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAAAGTTTCATCTGGTTATTGTCGATAACgg                                                                                                  <  2:164363/140‑1 (MQ=255)                                        aGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGc                                                                                                >  2:307796/1‑140 (MQ=255)                                              aTTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTg                                                                                          >  1:225509/1‑140 (MQ=255)                                               ttGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACgg                                                                                                  >  1:116901/1‑131 (MQ=255)                                               ttGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACgg                                                                                                  <  2:116901/131‑1 (MQ=255)                                               ttGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGa                                                                                         >  1:146979/1‑140 (MQ=255)                                               ttGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGa                                                                                         >  1:673863/1‑140 (MQ=255)                                               ttGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGGTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGa                                                                                         >  2:153570/1‑140 (MQ=255)                                                 gATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAgg                                                                                       >  2:499489/1‑140 (MQ=255)                                                 gATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAgg                                                                                       >  2:597087/1‑140 (MQ=255)                                                    cACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGc                                                                                    >  1:302279/1‑140 (MQ=255)                                                     aCCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACgg                                                                                                  <  2:158930/125‑1 (MQ=255)                                                     aCCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACgg                                                                                                  >  1:158930/1‑125 (MQ=255)                                                     aCCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCt                                                                                   >  2:553236/1‑140 (MQ=255)                                                     aCCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCt                                                                                   >  1:601177/1‑140 (MQ=255)                                                     aCCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTG‑‑AGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTgg                                                                                 >  2:148276/1‑140 (MQ=255)                                                              gcgcgcAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGa                                                                          >  2:719400/1‑140 (MQ=255)                                                                 cgcAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAAtt                                                                       >  2:600320/1‑140 (MQ=255)                                                                 cgcAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAAtt                                                                       <  2:601177/140‑1 (MQ=255)                                                                 cgcAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAAtt                                                                       >  1:484235/1‑140 (MQ=255)                                                                   cAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTc                                                                     >  1:394291/1‑140 (MQ=255)                                                                     gTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCgg                                                                   >  2:585155/1‑140 (MQ=255)                                                                      tGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCggg                                                                  <  1:398345/140‑1 (MQ=255)                                                                        ccGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGAt                                                                <  1:553236/140‑1 (MQ=255)                                                                           ttGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACTGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGACCTCTGAATTTCGGGAtgtg                                                             <  2:742762/140‑1 (MQ=255)                                                                                gCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTgcg                                                        <  2:394291/140‑1 (MQ=255)                                                                                gCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTgcg                                                        <  1:233000/140‑1 (MQ=255)                                                                                gCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTgcg                                                        >  1:720182/1‑140 (MQ=255)                                                                                 cACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTgcgc                                                       <  2:269151/140‑1 (MQ=255)                                                                                     tttGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTa                                                   >  2:250568/1‑140 (MQ=255)                                                                                      ttGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTAt                                                  >  1:446095/1‑140 (MQ=255)                                                                                       tGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTAtt                                                 <  2:484235/140‑1 (MQ=255)                                                                                              aTACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATCGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCg                                          >  1:259989/1‑140 (MQ=255)                                                                                                acaacaCCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCggg                                        <  1:499489/140‑1 (MQ=255)                                                                                                 caacaCCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCgggg                                       <  2:110079/140‑1 (MQ=255)                                                                                                  aacaCCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGc                                      <  2:306654/140‑1 (MQ=255)                                                                                                  aacaCCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGc                                      <  1:768798/140‑1 (MQ=255)                                                                                                   acacCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCt                                     <  1:585155/140‑1 (MQ=255)                                                                                                    cacCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCtt                                    <  2:673863/140‑1 (MQ=255)                                                                                                      ccTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGt                                  <  2:146979/140‑1 (MQ=255)                                                                                                        tGGCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTAt                                <  2:344378/140‑1 (MQ=255)                                                                                                         ggCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATg                               >  2:80949/1‑140 (MQ=255)                                                                                                         ggCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATg                               >  2:96832/1‑140 (MQ=255)                                                                                                         ggCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATg                               >  1:157021/1‑140 (MQ=255)                                                                                                         ggCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATg                               >  2:124755/1‑140 (MQ=255)                                                                                                         ggCTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATg                               >  1:200625/1‑140 (MQ=255)                                                                                                           cTGACAGGACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGaa                             <  1:101366/140‑1 (MQ=255)                                                                                                                 ggACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATa                           >  2:46841/1‑136 (MQ=255)                                                                                                                 ggACCGCGCGAAGCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATa                           <  1:46841/136‑1 (MQ=255)                                                                                                                             gCTGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCg           <  1:80949/140‑1 (MQ=255)                                                                                                                               tGGGCACGTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGGATACTTGTCCGGCATATCGcc         <  1:320319/140‑1 (MQ=255)                                                                                                                                      gTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTgg  <  1:148276/140‑1 (MQ=255)                                                                                                                                      gTTTATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTgg  <  1:307796/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GATGGGAATGGAGCGTATTGCCCCCACGTTTGCCGAGGTAGATGTATTGATCACCATGCTGGCGCGCAGTGCCGTTGGTGCACGTTTGACGGGATACAACACCTGGCTGACAGGACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGG  >  NC_000913/323051‑323323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑