Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,028,308	C→A	A155A (GCG→GCT)	fadA ←	3‑ketoacyl‑CoA thiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,028,308	0	C	A	100.0%	82.0 / NA	25	A155A (GCG→GCT)	fadA	3‑ketoacyl‑CoA thiolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/12);  total (13/12)

GGACGCCGTCGGCATCGTGACCACCGGTCGGGATGATTTCATTTTTAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGCGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCC  >  NC_000913/4028172‑4028440                                                                                                                                         |                                                                                                                                     ggACGCCGTCGGCATCGTGACCACCGGTCGGGATGATTTCATTTTTAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGcc                                                                                                                                   <  2:360975/140‑1 (MQ=255)    cGCCGTCGGCATCGTGACCACCGGTCGGGATGATTTCATTTTTAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGc                                                                                                                                >  2:625413/1‑140 (MQ=255)                 gTGACCACCGGTAGGGATGATTTCATTTTTAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTa                                                                                                                   <  1:760861/140‑1 (MQ=255)                   gACCACCGGTCGGGATGATTTCATTTTTAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAg                                                                                                                 <  1:248342/140‑1 (MQ=255)                               ggATGATTTCATTTTTAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGccc                                                                                                     <  2:721316/140‑1 (MQ=255)                                          tttttAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGc                                                                                          >  1:704287/1‑140 (MQ=255)                                             ttAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGAc                                                                                       >  1:192459/1‑140 (MQ=255)                                                 aTGCGGCCGACTGCGTGGGGGCCCGGGGGGGGGGGTGTGACCGCGCGGAAAAGCCTTCCTCCTTTTCACGGCTGATCCCGTGAATACGGGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTg                                                                                   <  2:188028/140‑1 (MQ=255)                                                     ggCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGc                                                                               >  2:133839/1‑140 (MQ=255)                                                       ccGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTc                                                                             >  1:30919/1‑140 (MQ=255)                                                       ccGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTc                                                                             <  1:671555/140‑1 (MQ=255)                                                           ttGCGGGGCGGCCCGGGCGGGGGCGTGTGACCGCGCGGAAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGc                                                                         <  2:123190/139‑1 (MQ=255)                                                             gCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCg                                                                       <  1:744801/140‑1 (MQ=255)                                                                tGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCgggg                                                                    <  1:303445/140‑1 (MQ=255)                                                                     gcCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGaaa                                                               >  1:492067/1‑140 (MQ=255)                                                                                ggCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGt                                                    >  1:524091/1‑140 (MQ=255)                                                                                ggCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGt                                                    >  2:561628/1‑140 (MQ=255)                                                                                         ccGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCg                                           <  2:524091/140‑1 (MQ=255)                                                                                             gcgGCAAGGGCATCCTGCATTTCACGGCTGATCCCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGcac                                       <  1:623712/140‑1 (MQ=255)                                                                                             gcgGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGcac                                       <  1:133839/140‑1 (MQ=255)                                                                                                 aaaaGGCATCCTGCATTTCACGGCTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGccatgtg                                   <  1:429579/139‑7 (MQ=255)                                                                                                                       gCTGATCCCGTGCATACGAGCCAGCTTTCCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCaa             <  2:669852/140‑1 (MQ=255)                                                                                                                        cTGATACCGTGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCAcgccgnnnnn            >  2:427860/1‑135 (MQ=255)                                                                                                                            tcccGTGCATACGAGCCAGCTTTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGa        <  2:233364/138‑1 (MQ=255)                                                                                                                                 tGCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGc   >  1:521545/1‑140 (MQ=255)                                                                                                                                  gCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGcc  >  1:472443/1‑140 (MQ=255)                                                                                                                                  gCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGcc  >  2:39855/1‑140 (MQ=255)                                                                                                                                  gCATACGAGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGcc  >  1:142317/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     GGACGCCGTCGGCATCGTGACCACCGGTCGGGATGATTTCATTTTTAAATGCGGCCGACTGCGTGGCGGCCCAGGCGCGGGCGTGTGACCGCGCGGCAAAGGCATCCTGCATTTCACGGCTGATACCGTGCATACGCGCCAGCATTTCTGCCGTTAAGCCCATCATGCCCGCCGCTTTGGCGACATTGCGGCTCAGGCCGGGGTGAAAATCGACGCCGTGACTCATCGGCACATGGCCCATATGCTCCACGCCGCCAACCAGACATGCC  >  NC_000913/4028172‑4028440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑