Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	335,274	A→G	intergenic (‑252/‑6)	yahC ← / → yahD	uncharacterized protein YahC/ankyrin repeat‑containing protein YahD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	335,274	0	A	G	100.0%	134.2 / NA	37	intergenic (‑252/‑6)	yahC/yahD	uncharacterized protein YahC/ankyrin repeat‑containing protein YahD
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (23/14);  total (23/14)

CCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAATGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCGATCGTCAGGGGAAAACGGCAATT  >  NC_000913/335138‑335408                                                                                                                                         |                                                                                                                                       ccGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAgtgt                                                                                                                                     >  1:113473/1‑140 (MQ=255)     acacTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAAt                                                                                                                                 <  1:489228/140‑1 (MQ=255)      cacTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTTTCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATg                                                                                                                                >  2:771282/1‑140 (MQ=255)      cacTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATg                                                                                                                                <  2:670026/140‑1 (MQ=255)      cacTGCGAAAAGATTGTTAAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATg                                                                                                                                <  2:564900/140‑1 (MQ=255)         tGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGt                                                                                                                             >  2:607346/1‑140 (MQ=255)         tGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGt                                                                                                                             >  1:205479/1‑140 (MQ=255)         tGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGt                                                                                                                             >  1:200804/1‑140 (MQ=255)         tGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGt                                                                                                                             >  1:525481/1‑140 (MQ=255)           cGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAGACAGCCACAAAAACCAGGGGAGTGTGAATGAGtat                                                                                                                           >  1:635839/1‑140 (MQ=255)                aGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATaaaaa                                                                                                                      >  2:348267/1‑140 (MQ=255)                   ttgttgAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCACCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATc                                                                                                                   >  1:334451/1‑140 (MQ=255)                       tgAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTAcc                                                                                                               >  1:230972/1‑140 (MQ=255)                        gAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCt                                                                                                              >  1:403032/1‑140 (MQ=255)                          atatTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGc                                                                                                            <  2:526636/140‑1 (MQ=255)                           tattatTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGcc                                                                                                           <  2:525481/140‑1 (MQ=255)                              tattCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGAc                                                                                                        >  1:754140/1‑140 (MQ=255)                                   aacaaAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACtattt                                                                                                   >  1:34273/1‑140 (MQ=255)                                   aacaaAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACtattt                                                                                                   <  1:446852/140‑1 (MQ=255)                                     caaAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACtatttat                                                                                                 >  2:394044/1‑140 (MQ=255)                                     caaAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACtatttat                                                                                                 >  2:697432/1‑140 (MQ=255)                                     caaAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACtatttat                                                                                                 >  1:241531/1‑140 (MQ=255)                                      aaaaaGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACtatttatt                                                                                                <  1:466840/140‑1 (MQ=255)                                       aaaaGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTg                                                                                               >  2:674269/1‑140 (MQ=255)                                        aaaGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTgg                                                                                              <  2:403032/140‑1 (MQ=255)                                        aaaGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTgg                                                                                              >  2:705492/1‑140 (MQ=255)                                             cGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGca                                                                                         <  2:181091/140‑1 (MQ=255)                                                                tttAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATa                                                                      <  1:348267/140‑1 (MQ=255)                                                                                    aGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTgcg                                                  <  1:607346/140‑1 (MQ=255)                                                                                              tGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCg                                        <  1:771282/140‑1 (MQ=255)                                                                                              tGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCg                                        <  1:271479/140‑1 (MQ=255)                                                                                                cTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGat                                      >  1:238551/1‑140 (MQ=255)                                                                                                        aaTTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATAc                              <  2:317509/140‑1 (MQ=255)                                                                                                        aaTTCATCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATAc                              >  1:30987/1‑140 (MQ=255)                                                                                                             aTCGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCg                         >  2:380548/1‑140 (MQ=255)                                                                                                               cGAAAACAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCGAt                       >  1:648550/1‑140 (MQ=255)                                                                                                                  aaaCAGCCACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCGATCGt                    >  1:100522/1‑140 (MQ=255)                                                                                                                        ccACAAAAACCAGGGGAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCGATCGTCAgggg              >  1:614198/1‑140 (MQ=255)                                                                                                                                    ggggAGTGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCGATCGTCAGGGGAAAACGGCAAtt  >  2:637217/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CCGCACACTGCGAAAAGATTGTTGAATATTATTCAACAAAAAGGCGAGATACGGCGAATTAAATTTAAGCGGGTTCTTTTATCAGATTATCGTTGCTATTCTCAATTCATCGAAAACAGCCACAAAAACCAGGGGAATGTGAATGAGTATAAAAAATCTACCTGCCGACTATTTATTGGCTGCACAACAGGGTGATATCGATAAAGTAAAAACCTGCCTTGCGCTGGGTGTCGATATTAATACCTGCGATCGTCAGGGGAAAACGGCAATT  >  NC_000913/335138‑335408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑