Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,548,920	G→T	S507I (AGC→ATC)	fdnG →	formate dehydrogenase N subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,548,920	0	G	T	100.0%	99.4 / NA	29	S507I (AGC→ATC)	fdnG	formate dehydrogenase N subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/11);  total (18/11)

GCCTGCCAGGTTATCTGACGCTGCCGTCAGAAAAACAGGTTGATTTGCAGTCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGG  >  NC_000913/1548788‑1549052                                                                                                                                     |                                                                                                                                     gCCTGCCAGGTTATCTGACGCTGCCGTCAGAAAAACAGGTTGATTTGCAGTCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCtgatg                                                                                                                               >  2:26888/1‑140 (MQ=255)              tCTGACGCTGCCGTCAGAAAAACAGGTTGATTTGCAGTCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATg                                                                                                                  >  2:734236/1‑140 (MQ=255)                    gCTGCCGTCAGAAAAACAGGTTGATTTGCAGTCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATg                                                                                                            >  2:85486/1‑140 (MQ=255)                       gCCGTCAGAAAAACAGGTTGATTTGCAGTCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCcg                                                                                                         <  1:40403/140‑1 (MQ=255)                        ccGTCAGAAAAACAGGTTGATTTGCAGTCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCcgc                                                                                                        >  2:149776/1‑140 (MQ=255)                                         tGATTTGCAGTCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACt                                                                                       >  1:63663/1‑140 (MQ=255)                                                   tCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGa                                                                             <  1:192034/140‑1 (MQ=255)                                                           ggAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGc                                                                     >  1:381217/1‑140 (MQ=255)                                                                 gAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGt                                                               >  1:303752/1‑140 (MQ=255)                                                                 gAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGt                                                               >  2:402531/1‑140 (MQ=255)                                                                  aaCACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTg                                                              >  1:413453/1‑140 (MQ=255)                                                                           aaaGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGAc                                                     >  1:253786/1‑140 (MQ=255)                                                                            aaGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGAcc                                                    >  1:640725/1‑140 (MQ=255)                                                                                  cGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTacgac                                              <  2:640725/140‑1 (MQ=255)                                                                                           aTCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAg                                     <  1:85486/140‑1 (MQ=255)                                                                                                gTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTAttt                                <  1:734236/140‑1 (MQ=255)                                                                                                gTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCCGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTAttt                                >  1:472557/1‑140 (MQ=255)                                                                                                 tGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTc                               >  1:24857/1‑140 (MQ=255)                                                                                                        ctGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACatga                        <  1:402531/140‑1 (MQ=255)                                                                                                        ctGGAGCAACTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACatga                        <  2:381217/140‑1 (MQ=255)                                                                                                                aCTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGATAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGaa                >  1:1766/1‑140 (MQ=255)                                                                                                                aCTATCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGaa                >  1:155523/1‑140 (MQ=255)                                                                                                                   aTCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGc             <  1:230256/140‑1 (MQ=255)                                                                                                                    tCCGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCa            <  2:253786/140‑1 (MQ=255)                                                                                                                     ccGAAGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCaa           >  1:587011/1‑140 (MQ=255)                                                                                                                         aGTTCTTCGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTGTGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTc       <  1:313662/140‑1 (MQ=255)                                                                                                                            tcttcGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCAcc    >  2:390941/1‑140 (MQ=255)                                                                                                                             cttcGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCg   <  1:26888/140‑1 (MQ=255)                                                                                                                              ttcGTTATCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCgg  >  1:657018/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                     GCCTGCCAGGTTATCTGACGCTGCCGTCAGAAAAACAGGTTGATTTGCAGTCGTATCTGGAAGCGAACACGCCGAAAGCGACGCTGGCTGATCAGGTGAACTACTGGAGCAACTATCCGAAGTTCTTCGTTAGCCTGATGAAATCTTTCTATGGCGATGCCGCGCAGAAAGAGAACAACTGGGGCTATGACTGGCTGCCGAAGTGGGACCAGACCTACGACGTCATCAAGTATTTCAACATGATGGATGAAGGCAAAGTCACCGG  >  NC_000913/1548788‑1549052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑