Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,676,155	G→T	A69E (GCA→GAA)	pntB ←	pyridine nucleotide transhydrogenase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,676,155	0	G	T	100.0%	172.0 / NA	49	A69E (GCA→GAA)	pntB	pyridine nucleotide transhydrogenase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/22);  total (27/22)

GTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGTTTCG  >  NC_000913/1676018‑1676287                                                                                                                                          |                                                                                                                                     gTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTcc                                                                                                                                    >  2:727976/1‑140 (MQ=255)  tGCCTTTCCCGCGTCATGATGCAGATAGCTGTTAAAGCCTGCCAGCACTGCCGCAAGACCCACGGAGCTTTAAAAGATCGCAACCAGTTCTGACATTTCGGTCACTGCAACTTTCTGCTCCAGACGGATACCAATTGccc                                                                                                                                   <  2:630350/140‑1 (MQ=255)  tGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTccc                                                                                                                                   <  2:325285/140‑1 (MQ=255)    ccATTCCCGCGTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCcca                                                                                                                                 <  2:679199/140‑1 (MQ=255)            gcgTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGAc                                                                                                                         >  1:184071/1‑140 (MQ=255)            gcgTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGAc                                                                                                                         >  1:281049/1‑140 (MQ=255)                cATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATc                                                                                                                     <  1:616449/140‑1 (MQ=255)                          aTAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGa                                                                                                           >  2:766007/1‑140 (MQ=255)                          aTAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGa                                                                                                           >  1:645854/1‑140 (MQ=255)                           tAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGAt                                                                                                          <  1:160641/140‑1 (MQ=255)                            aGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATc                                                                                                         >  2:212246/1‑140 (MQ=255)                               tGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAg                                                                                                      <  1:19347/140‑1 (MQ=255)                                gTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGc                                                                                                     <  2:689245/140‑1 (MQ=255)                                 ttAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGcc                                                                                                    >  2:258546/1‑140 (MQ=255)                                 ttAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGcc                                                                                                    <  1:740245/140‑1 (MQ=255)                                      gCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACTAATGACCATCGCCAGCAAGATCCAGCCAACAt                                                                                               <  1:444123/140‑1 (MQ=255)                                      gCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACAt                                                                                               <  1:212246/140‑1 (MQ=255)                                      gCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACAt                                                                                               <  1:2241/140‑1 (MQ=255)                                          aCCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTAcc                                                                                           <  1:429417/140‑1 (MQ=255)                                           ccAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTAATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTAccc                                                                                          >  2:559166/1‑140 (MQ=255)                                                  tGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTAATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCg                                                                                   <  1:559166/140‑1 (MQ=255)                                                   gccgccAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCACTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCgg                                                                                  <  1:745319/140‑1 (MQ=255)                                                      gccAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTcc                                                                               >  1:468824/1‑140 (MQ=255)                                                         aGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCaaa                                                                            >  2:220729/1‑140 (MQ=255)                                                           aCCCCCGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCCCCAATGACCATCGCCAGCAAGATCCAGCCAACATTCCCCGTATCCGGTCCaaaaa                                                                          <  2:129024/140‑1 (MQ=255)                                                                    gCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGa                                                                 >  2:604223/1‑140 (MQ=255)                                                                             gATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCaa                                                        >  2:549518/1‑140 (MQ=255)                                                                             gATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCaa                                                        >  2:738245/1‑140 (MQ=255)                                                                             gATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCaa                                                        >  2:468842/1‑140 (MQ=255)                                                                               tCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATc                                                      >  1:308832/1‑140 (MQ=255)                                                                                  ccaccaGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGcc                                                   >  1:580786/1‑140 (MQ=255)                                                                                  ccaccaGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGcc                                                   >  2:758249/1‑140 (MQ=255)                                                                                  ccaccaGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGcc                                                   >  2:504232/1‑140 (MQ=255)                                                                                       aGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATccc                                              >  1:132044/1‑140 (MQ=255)                                                                                       aGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATccc                                              >  2:405472/1‑140 (MQ=255)                                                                                       aGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATccc                                              >  1:590250/1‑140 (MQ=255)                                                                                       aGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATccc                                              >  2:261131/1‑140 (MQ=255)                                                                                       aGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATccc                                              >  2:24415/1‑140 (MQ=255)                                                                                         ttCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCgg                                            <  1:616688/140‑1 (MQ=255)                                                                                            tGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGa                                         <  1:95002/140‑1 (MQ=255)                                                                                                 tttCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCg                                    <  2:763252/140‑1 (MQ=255)                                                                                                 tttCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCg                                    <  2:468824/140‑1 (MQ=255)                                                                                                 tttCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCg                                    <  2:184071/140‑1 (MQ=255)                                                                                                  ttCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGa                                   <  2:281049/140‑1 (MQ=255)                                                                                                   tCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGaa                                  >  2:121268/1‑140 (MQ=255)                                                                                                   tCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGaa                                  >  1:655069/1‑140 (MQ=255)                                                                                                                 tttCTTCGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGc                    <  1:261131/140‑1 (MQ=255)                                                                                                                    cttcGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCgag                 <  1:258546/140‑1 (MQ=255)                                                                                                                     ttcGCCAGACGGATACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCgaga                >  1:323282/1‑140 (MQ=255)                                                                                                                                 aTACCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGttt    >  2:766349/1‑140 (MQ=255)                                                                                                                                   aCCAATTTCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGTTTCg  <  1:758249/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     GTGCCATTCCCGCGTCATGATGCAGATAGCTGTTAAAGCCAACCAGCACTGCCGCCAGACCCACGAAGCTATGCAGGATCGCCACCAGTTCTGGCATTTCGGTCATTTCAACTTTCTTCGCCAGACGGATACCAATTGCCCCACCAATGACCATCGCCAGCAAGATCCAGCCAACATTACCCGTATCCGGTCCAAAAATGGTTGCGATTAACGCAATCGCCATCCCGGCGATACCGAAGTTGTTACCCTGGCGAGACGTTTCATGTTTCG  >  NC_000913/1676018‑1676287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑