| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,606,295 | A→G | T66A (ACC→GCC) | lsrF → | 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,606,295 | 0 | A | G | 96.8% | 221.4 / ‑2.7 | 63 | T66A (ACC→GCC) | lsrF | 3‑hydroxy‑2,4‑pentadione 5‑phosphate thiolase |
| Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (24/37); total (25/38) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
CTTTTACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGC > NC_000913/1606167‑1606431 | cTTTTACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGaa < 2:116765/140‑1 (MQ=255)cTTTTACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGaa < 1:250971/140‑1 (MQ=255) ttACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGc > 1:572461/1‑140 (MQ=255) ttcccTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCa < 1:258262/138‑1 (MQ=255) tACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCa < 1:84193/140‑1 (MQ=255) tACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCa < 1:182343/140‑1 (MQ=255) tACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCa < 2:905215/140‑1 (MQ=255) tACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCa < 2:149374/140‑1 (MQ=255) tACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCa < 2:585147/140‑1 (MQ=255) tACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCa < 2:572461/140‑1 (MQ=255) tACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCAGGACTTGAACGCa < 1:557789/140‑1 (MQ=255) aCCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCAt < 1:278404/140‑1 (MQ=255) aCCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCAt < 1:664374/140‑1 (MQ=255) aCCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCAt < 2:388534/140‑1 (MQ=255) aCCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCAt < 1:532500/140‑1 (MQ=255) cccTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCAtt < 1:179044/140‑1 (MQ=255) cccTGAAAGGTTGCGGTGCGCTAGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCAtt < 1:587346/140‑1 (MQ=255) aaaGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGatat > 2:553483/1‑140 (MQ=255) ttGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGatat < 2:208399/135‑1 (MQ=255) ttGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGatat > 1:208399/1‑135 (MQ=255) tGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAatat > 2:816065/1‑140 (MQ=255) tGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAatat > 2:265614/1‑140 (MQ=255) cGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCg > 2:465180/1‑140 (MQ=255) gcgcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGcccg < 2:175721/140‑2 (MQ=255) gcgcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGcccc < 2:622350/140‑1 (MQ=255) cgcTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCTAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCg < 2:858796/140‑1 (MQ=255) ggATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGt < 2:49163/140‑1 (MQ=255) gggAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGTTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAAc > 1:540934/1‑140 (MQ=255) gggAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGGAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAAc > 2:996020/1‑140 (MQ=255) aTGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGc < 1:996020/140‑1 (MQ=255) tGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc > 1:787915/1‑140 (MQ=255) tGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc > 2:282634/1‑140 (MQ=255) tGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc > 1:551020/1‑140 (MQ=255) gCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGTTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCg < 2:540934/140‑1 (MQ=255) gCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCg < 2:910494/140‑1 (MQ=255) aGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGAt < 1:946020/140‑1 (MQ=255) aGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGAt < 1:553483/140‑1 (MQ=255) atTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTAcgcgcg < 1:728569/140‑1 (MQ=255) aaaaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTgcg > 1:756579/1‑140 (MQ=255) aaaaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTgcg > 1:731232/1‑140 (MQ=255) aaaaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTgcg < 2:731232/140‑1 (MQ=255) aaaaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTgcg > 2:721913/1‑140 (MQ=255) aaaCGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTgcgc > 2:1034381/1‑140 (MQ=255) cGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTcccc < 2:551020/140‑1 (MQ=255) cGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTcccc < 1:21666/140‑1 (MQ=255) gATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTg > 2:767179/1‑140 (MQ=255) gATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTg > 2:459440/1‑140 (MQ=255) aTGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGc < 1:459440/140‑1 (MQ=255) tGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCg < 1:236496/140‑1 (MQ=255) tGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCg < 1:307513/140‑1 (MQ=255) gCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGa > 2:682694/1‑140 (MQ=255) tttGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAg < 1:682694/140‑1 (MQ=255) tGACCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGc < 1:465180/140‑1 (MQ=255) aCCATGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCg > 2:64154/1‑140 (MQ=255) aTGGTTATTTTCAGGGACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCggtg > 1:820175/1‑140 (MQ=255) gggACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGt < 1:1034381/140‑1 (MQ=255) ggACCGACTGCCGGACTTGAACGCTTTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTc > 2:559553/1‑140 (MQ=255) ggACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTc < 2:756579/140‑1 (MQ=255) ggACCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCGGTTTGACCATGCCGATGTATTAATGGGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGCCCAATAGCCCGGTGGTACTGCGGGCgcc > 2:453082/1‑138 (MQ=255) aCCGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAg > 1:50412/1‑140 (MQ=255) cGACTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGt > 2:925971/1‑140 (MQ=255) aCTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGc < 1:282634/140‑1 (MQ=255) aCTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGc > 2:447075/1‑140 (MQ=255) aCTGCCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGc < 1:517103/140‑1 (MQ=255) | CTTTTACCCTGAAAGGTTGCGGTGCGCTGGATTGGGGAATGCAGTCACGCTTATCGCGGATATTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGCGCGGCATTTTGCGCAGCGTAGTTCCCCCTGCGACCAATAGGCCGGTGGTACTGCGGGCGTCAGGTGC > NC_000913/1606167‑1606431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |