Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,688,480	T→A	intergenic (‑103/‑96)	fumA ← / → manA	fumarase A/mannose‑6‑phosphate isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,688,480	0	T	A	100.0%	255.6 / NA	71	intergenic (‑103/‑96)	fumA/manA	fumarase A/mannose‑6‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (37/34);  total (37/34)

AGGAGCCTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACTGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGG  >  NC_000913/1688345‑1688616                                                                                                                                        |                                                                                                                                         aGGAGCCTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAgaga                                                                                                                                      <  2:882750/140‑1 (MQ=255) aGGAGCCTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAgaga                                                                                                                                      <  2:719713/140‑1 (MQ=255)  ggAGCCTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGAc                                                                                                                                     <  1:399630/140‑1 (MQ=255)  ggAGCCTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGAc                                                                                                                                     <  2:65697/140‑1 (MQ=255)     gCCTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAg                                                                                                                                  >  1:538993/1‑140 (MQ=255)      ccTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGt                                                                                                                                 >  2:389967/1‑140 (MQ=255)       cTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTa                                                                                                                                >  2:483859/1‑140 (MQ=255)        tGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTAc                                                                                                                               >  1:750483/1‑140 (MQ=255)        tGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTAc                                                                                                                               >  2:289734/1‑140 (MQ=255)        tGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTAc                                                                                                                               >  2:173469/1‑140 (MQ=255)                 aaGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTg                                                                                                                      <  1:289734/140‑1 (MQ=255)                  aGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGc                                                                                                                     >  2:712675/1‑140 (MQ=255)                    ggTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCgg                                                                                                                   <  1:389967/140‑1 (MQ=255)                         gtttgACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCa                                                                                                              >  1:871989/1‑140 (MQ=255)                          tttgACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAg                                                                                                             <  1:682238/140‑1 (MQ=255)                          tttgACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAg                                                                                                             <  1:559097/140‑1 (MQ=255)                            tgACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGt                                                                                                           <  1:978442/140‑1 (MQ=255)                                aTTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACt                                                                                                       <  1:153748/140‑1 (MQ=255)                                   gTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTcc                                                                                                    >  1:116880/1‑140 (MQ=255)                                    ttCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTccc                                                                                                   >  2:1000045/1‑140 (MQ=255)                                       tctcACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTa                                                                                                <  2:871989/140‑1 (MQ=255)                                            cTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGAtt                                                                                           <  2:364907/140‑1 (MQ=255)                                            cTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGAtt                                                                                           <  1:173469/140‑1 (MQ=255)                                              tACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCt                                                                                         <  2:116880/140‑1 (MQ=255)                                              tACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCt                                                                                         >  2:201543/1‑140 (MQ=255)                                              tACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCt                                                                                         >  2:104214/1‑140 (MQ=255)                                                   ccTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCtt                                                                                    >  2:817989/1‑140 (MQ=255)                                                     tggtttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTa                                                                                  >  1:564174/1‑140 (MQ=255)                                                     tggtttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTa                                                                                  >  2:680082/1‑140 (MQ=255)                                                      ggtttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTaa                                                                                 >  1:659453/1‑140 (MQ=255)                                                      ggtttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTaa                                                                                 <  1:201543/140‑1 (MQ=255)                                                       gtttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAAt                                                                                >  2:223275/1‑140 (MQ=255)                                                       gtttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAAt                                                                                >  1:830475/1‑140 (MQ=255)                                                        tttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTT‑AAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAg                                                                              <  2:453146/140‑1 (MQ=255)                                                         ttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAg                                                                              <  1:104214/140‑1 (MQ=255)                                                         ttggttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAg                                                                              <  1:483859/140‑1 (MQ=255)                                                             ttATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACAC‑TTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGa                                                                         >  2:346661/1‑140 (MQ=255)                                                                  ctctGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTaa                                                                     >  1:328811/1‑140 (MQ=255)                                                                       ggCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTcc                                                                >  1:612827/1‑140 (MQ=255)                                                                           ggTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACAt                                                            <  1:315424/140‑1 (MQ=255)                                                                           ggTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACAt                                                            <  2:830475/140‑1 (MQ=255)                                                                           ggTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACAt                                                            <  1:680082/140‑1 (MQ=255)                                                                            gtgtTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACAtt                                                           >  2:511196/1‑140 (MQ=255)                                                                                tCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTaaaa                                                       <  2:750483/140‑1 (MQ=255)                                                                                tCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTaaaa                                                       <  2:564174/140‑1 (MQ=255)                                                                                tCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTaaaa                                                       <  2:538993/140‑1 (MQ=255)                                                                                tCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTaaaa                                                       <  1:1000045/140‑1 (MQ=255)                                                                                       cccTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGAt                                                >  2:906069/1‑140 (MQ=255)                                                                                            ttAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATc                                           >  1:504817/1‑140 (MQ=255)                                                                                                      gTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCaaaaa                                  <  2:179696/139‑1 (MQ=255)                                                                                                      gTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCaaaaa                                  >  1:179696/1‑139 (MQ=255)                                                                                                      gTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAAc                                 >  1:434170/1‑140 (MQ=255)                                                                                                      gTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAAc                                 >  2:898660/1‑140 (MQ=255)                                                                                                      gTAACAATATCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAAc                                 >  2:703248/1‑140 (MQ=255)                                                                                                      gTAACAATATACTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAAc                                 >  2:201874/1‑140 (MQ=255)                                                                                                               tCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACt                        <  2:257260/140‑1 (MQ=255)                                                                                                               tCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACt                        >  1:179622/1‑140 (MQ=255)                                                                                                               tCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACt                        <  2:208025/140‑1 (MQ=255)                                                                                                               tCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACt                        <  2:179622/140‑1 (MQ=255)                                                                                                               tCCTACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACt                        <  1:898660/140‑1 (MQ=255)                                                                                                                  tACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAg                     >  1:766546/1‑140 (MQ=255)                                                                                                                  tACACACTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTCATCATGCAAAAACTCATTAACTCAg                     >  1:313423/1‑140 (MQ=255)                                                                                                                    cacacTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTg                   <  1:906069/140‑1 (MQ=255)                                                                                                                     acacTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGc                  <  2:504817/140‑1 (MQ=255)                                                                                                                     acacTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGc                  >  2:841300/1‑140 (MQ=255)                                                                                                                       acTTTTTTAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCaa                >  2:1002332/1‑140 (MQ=255)                                                                                                                         ttttttAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCaaaa              >  1:38802/1‑140 (MQ=255)                                                                                                                         ttttttAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCaaaa              >  2:868787/1‑140 (MQ=255)                                                                                                                         ttttttAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCaaaa              <  2:434170/140‑1 (MQ=255)                                                                                                                         ttttttAACAAAAACAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCaaaa              >  2:19870/1‑140 (MQ=255)                                                                                                                                     aaCAGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTgggg  <  1:511196/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         AGGAGCCTGATAATGAAAGGGTTTGTTTGACATTGTTCTCTCACTTACTGCCTGGTTTGGTTATGCTCTGGGCGGGTGTTCCGTTGCCCTGTTAAAAGCGAGTAACAATATCCTACACACTTTTTTAACAAAAACTGAGACTAGTACGACTTTTTGCGGCTCCAGGTTACTTCCCGTAGGATTCTTGCTTTAATAGTGGGATTAATTTCCACATTAAAACAGGGATTGATCATGCAAAAACTCATTAACTCAGTGCAAAACTATGCCTGGGG  >  NC_000913/1688345‑1688616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑