Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,720,181	A→C	V102V (GTA→GTC)	slyB →	outer membrane lipoprotein SlyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,720,181	0	A	C	100.0%	208.1 / NA	57	V102V (GTA→GTC)	slyB	outer membrane lipoprotein SlyB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/26);  total (31/26)

CCAACGTTATCGGTGCAATTGGCGGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGG  >  NC_000913/1720051‑1720315                                                                                                                                   |                                                                                                                                       ccAACGTTATCGGTGCAATTGGCGGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCa                                                                                                                               <  2:251070/140‑1 (MQ=255)        tATCGGTGCAATTGGCGGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTTTCTGGCTACTGCAGCAAGCGCTGTTGCAGGTGGCGGAGCTGGGCAGGGCGTCCAGAGTGCAATGaaaa                                                                                                                        >  2:79734/1‑138 (MQ=255)        tATCGGTGCAATTGGCGGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACa                                                                                                                        >  1:190010/1‑140 (MQ=255)             gTGCAATTGGCGGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACg                                                                                                                   >  1:958452/1‑140 (MQ=255)              tGCAATTGGCGGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGGGTGCAATGAACAAAACGc                                                                                                                  >  2:878318/1‑140 (MQ=255)                        ggTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGa                                                                                                        >  2:885445/1‑140 (MQ=255)                        ggTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGa                                                                                                        >  2:19497/1‑140 (MQ=255)                        ggTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGa                                                                                                        >  1:7305/1‑140 (MQ=255)                        ggTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGa                                                                                                        >  2:23362/1‑140 (MQ=255)                           gCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCt                                                                                                     >  1:817476/1‑140 (MQ=255)                                tCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaaa                                                                                                <  1:79734/140‑1 (MQ=255)                                tCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGaaa                                                                                                <  1:74763/140‑1 (MQ=255)                                 cTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAAt                                                                                               <  2:958452/140‑1 (MQ=255)                                    ggtggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGCGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCg                                                                                            >  2:1019880/1‑140 (MQ=255)                                      tggtTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGATCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTa                                                                                          <  1:1002094/140‑1 (MQ=255)                                         tttCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAg                                                                                       >  2:452453/1‑140 (MQ=255)                                         tttCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAg                                                                                       >  2:856260/1‑140 (MQ=255)                                           tCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc                                                                                     <  2:974247/140‑1 (MQ=255)                                           tCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGAc                                                                                     <  1:885445/140‑1 (MQ=255)                                            ccTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACg                                                                                    <  2:234222/140‑1 (MQ=255)                                            ccTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACg                                                                                    <  1:1006927/140‑1 (MQ=255)                                               ggggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATg                                                                                 <  1:1019880/140‑1 (MQ=255)                                                 ggAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGt                                                                               <  2:802679/140‑1 (MQ=255)                                                   aaTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTaa                                                                             <  1:1703/140‑1 (MQ=255)                                                   aaTACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTaa                                                                             <  2:903193/140‑1 (MQ=255)                                                       cTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATAcc                                                                         >  1:779209/1‑140 (MQ=255)                                                         gTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcat                                                                       <  1:350559/140‑1 (MQ=255)                                                          ttGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatc                                                                      >  2:689966/1‑140 (MQ=255)                                                            ggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatcat                                                                    >  1:534882/1‑140 (MQ=255)                                                            ggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatcat                                                                    >  2:969539/1‑140 (MQ=255)                                                            ggtggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACcatcat                                                                    >  2:400473/1‑140 (MQ=255)                                                              tggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtgg                                                                  <  1:878318/140‑1 (MQ=255)                                                              tggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtgg                                                                  <  2:190010/140‑1 (MQ=255)                                                               ggCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtggt                                                                 >  1:773613/1‑140 (MQ=255)                                                                 cGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCAtggtgg                                                               >  1:667882/1‑140 (MQ=255)                                                                  ggAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATggtggt                                                              >  2:409600/1‑140 (MQ=255)                                                                     aCCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACa                                                           <  2:52189/140‑1 (MQ=255)                                                                       cGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGa                                                         <  2:779209/140‑1 (MQ=255)                                                                         ggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGaaa                                                       >  2:243961/1‑140 (MQ=255)                                                                         ggCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGaaa                                                       <  1:856260/140‑1 (MQ=255)                                                                            gTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACaa                                                    <  1:400473/140‑1 (MQ=255)                                                                              tctcTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAgg                                                  <  2:728098/140‑1 (MQ=255)                                                                                    gCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAacac                                            >  1:868844/1‑140 (MQ=255)                                                                                    gCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAacac                                            >  2:987272/1‑140 (MQ=255)                                                                                         tGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGtt                                       >  1:814197/1‑140 (MQ=255)                                                                                              cagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTtctct                                  >  2:667055/1‑140 (MQ=255)                                                                                              cagGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTtctct                                  >  1:189243/1‑140 (MQ=255)                                                                                               agGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTtctctc                                 <  2:49753/140‑1 (MQ=255)                                                                                                     tGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGcc                           >  2:455140/1‑140 (MQ=255)                                                                                                     tGTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGcc                           >  1:680359/1‑140 (MQ=255)                                                                                                      gTTGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCa                          <  1:969539/140‑1 (MQ=255)                                                                                                        tGCAGGTGGCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAAc                        <  1:243961/140‑1 (MQ=255)                                                                                                                gCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTa                <  2:7305/140‑1 (MQ=255)                                                                                                                gCGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTa                <  2:868844/140‑1 (MQ=255)                                                                                                                 cGTAGCTGGTCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTAc               >  2:213721/1‑140 (MQ=255)                                                                                                                          tCAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCa      >  1:835396/1‑140 (MQ=255)                                                                                                                           cAGGGCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCaa     >  2:925808/1‑140 (MQ=255)                                                                                                                              ggCGTCCAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATgg  >  1:983488/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                       CCAACGTTATCGGTGCAATTGGCGGTGCTGTTCTTGGTGGTTTCCTGGGGAATACTGTTGGTGGCGGAACCGGGCGTTCTCTGGCTACTGCAGCAGGCGCTGTTGCAGGTGGCGTAGCTGGTCAGGGCGTACAGAGTGCAATGAACAAAACGCAGGGTGTCGAGCTGGAAATTCGTAAAGACGATGGTAATACCATCATGGTGGTACAGAAACAAGGCAACACTCGTTTCTCTCCGGGCCAACGTGTCGTACTGGCCAGCAATGG  >  NC_000913/1720051‑1720315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑