Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,769,719	G→T	R6L (CGC→CTC)	yfjR →	CP4‑57 prophage; putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,769,719	0	G	T	100.0%	253.5 / NA	72	R6L (CGC→CTC)	yfjR	CP4‑57 prophage; putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (42/30);  total (42/30)

CGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGA  >  NC_000913/2769583‑2769855                                                                                                                                         |                                                                                                                                         cGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAActccg                                                                                                                                       >  2:960372/1‑140 (MQ=255)           ggCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCgg                                                                                                                             >  2:798842/1‑140 (MQ=255)            gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCggc                                                                                                                            >  1:903397/1‑140 (MQ=255)            gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCggc                                                                                                                            >  2:877318/1‑140 (MQ=255)            gCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCggc                                                                                                                            <  1:506049/140‑1 (MQ=255)             cTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCggct                                                                                                                           >  2:617/1‑140 (MQ=255)                 tGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCggctggct                                                                                                                       >  1:174063/1‑140 (MQ=255)                 tGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCggct                                                                                                                           >  2:373823/1‑136 (MQ=255)                 tGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCggct                                                                                                                           <  1:373823/136‑1 (MQ=255)                   cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCCTGACCGGCTggctgt                                                                                                                     <  2:242223/140‑1 (MQ=255)                   cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTggctgt                                                                                                                     <  2:251713/140‑1 (MQ=255)                   cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTggctgt                                                                                                                     <  1:233367/140‑1 (MQ=255)                   cAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTggctgt                                                                                                                     <  2:803547/140‑1 (MQ=255)                      aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTggctgtcag                                                                                                                  >  1:131651/1‑140 (MQ=255)                      aaaTAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTggctgtcag                                                                                                                  >  2:332766/1‑140 (MQ=255)                           aaaaaTAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTggctgtcaggctgt                                                                                                             <  1:798842/140‑1 (MQ=255)                                        gCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAg                                                                                                <  2:787442/140‑1 (MQ=255)                                            tGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGt                                                                                            <  1:198866/140‑1 (MQ=255)                                                   aCCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTg                                                                                     >  2:490478/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACTTCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                <  2:800556/140‑1 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCTGTCTGGTTGCGGGt                                                                                <  1:275794/140‑1 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  2:953833/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  2:199716/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  2:254434/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  1:240289/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  2:832005/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                <  1:832356/140‑1 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  2:524701/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  1:520907/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  1:516531/1‑140 (MQ=255)                                                        gTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGt                                                                                >  1:337193/1‑140 (MQ=255)                                                         tCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTg                                                                               >  1:167910/1‑140 (MQ=255)                                                         tCCGCCCCCCTCTTTAAAGCAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTg                                                                               >  1:544069/1‑140 (MQ=255)                                                          ccGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGa                                                                              >  2:862545/1‑140 (MQ=255)                                                          ccGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGACTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGa                                                                              <  2:92024/140‑1 (MQ=255)                                                             ccccccTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAAc                                                                           >  1:936827/1‑140 (MQ=255)                                                                   tctTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAg                                                                     <  1:953833/140‑1 (MQ=255)                                                                            tAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCaa                                                            <  2:288493/140‑1 (MQ=255)                                                                              aTCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAAc                                                          >  2:305855/1‑140 (MQ=255)                                                                              aTCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAAc                                                          >  1:97764/1‑140 (MQ=255)                                                                               tCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACt                                                         >  1:318010/1‑140 (MQ=255)                                                                               tCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACt                                                         >  2:913231/1‑140 (MQ=255)                                                                                   atcatTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTggcg                                                     >  2:538869/1‑140 (MQ=255)                                                                                    tcatTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACcggcgg                                                    <  2:240289/140‑1 (MQ=255)                                                                                     catTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTggcggc                                                   <  2:174063/140‑1 (MQ=255)                                                                                     catTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTggcggc                                                   <  1:254434/140‑1 (MQ=255)                                                                                       ttttCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTg                                                 <  2:97764/140‑1 (MQ=255)                                                                                        tttCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGa                                                >  2:879482/1‑140 (MQ=255)                                                                                         ttCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAg                                               >  1:451625/1‑140 (MQ=255)                                                                                          tcagtcagTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGt                                              >  1:213011/1‑140 (MQ=255)                                                                                             gtcagtTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTg                                           <  1:489019/140‑1 (MQ=255)                                                                                             gtcagtTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTg                                           <  1:630235/140‑1 (MQ=255)                                                                                              tcagtTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTgg                                          >  1:683366/1‑140 (MQ=255)                                                                                                 gtTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCgt                                       >  1:956613/1‑140 (MQ=255)                                                                                                  ttAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCgtg                                      >  1:117845/1‑140 (MQ=255)                                                                                                   tAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCgtgt                                     <  2:167910/140‑1 (MQ=255)                                                                                                    aaCTTTCCTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTc                                    <  1:877318/140‑1 (MQ=255)                                                                                                          ccTGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTgcg                              >  2:42990/1‑140 (MQ=255)                                                                                                            tGGAGAATCTCTCATGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGAGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCa                            >  2:956845/1‑140 (MQ=255)                                                                                                                          tGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTgcgg                    >  2:435659/1‑134 (MQ=255)                                                                                                                          tGACACAGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTgcgg                    <  1:435659/134‑1 (MQ=255)                                                                                                                              acaGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTc          >  1:890593/1‑140 (MQ=255)                                                                                                                                aGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGt        >  2:62464/1‑140 (MQ=255)                                                                                                                                aGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGt        >  1:265840/1‑140 (MQ=255)                                                                                                                                aGGCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGt        >  1:957692/1‑140 (MQ=255)                                                                                                                                 ggCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTg       >  2:984058/1‑140 (MQ=255)                                                                                                                                  gCAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGa      >  2:631247/1‑140 (MQ=255)                                                                                                                                   cAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGaa     <  2:520907/140‑1 (MQ=255)                                                                                                                                   cAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGaa     <  1:617/140‑1 (MQ=255)                                                                                                                                   cAGAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGaa     <  1:42990/140‑1 (MQ=255)                                                                                                                                     gAACTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACg   <  1:862545/140‑1 (MQ=255)                                                                                                                                      aaCTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGa  <  1:960372/140‑1 (MQ=255)                                                                                                                                      aaCTCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGa  <  1:879482/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CGTTACATCTGGCTTTTGCAGAAATAAAAAATAGTTTCTGCGTTGTCCATACCCTGTCCGCCCCCCTCTTTAAAGTAATCACATCATTTTCAGTCAGTTAACTTTCCTGGAGAATCTCTCATGACACAGGCAGAACGCCGCCATGACCGGCTGGCTGTCAGGCTGTCACTGATAATCAGCCGTCTGGTTGCGGGTGAAACGTTGAGCGTGCGCAAACTGGCGGCTGAGTTTGGCGTGTCAGTGCGCACGCTGCGGCGTGATTTTCGTGAACGA  >  NC_000913/2769583‑2769855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑