Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,835,689 C→A A579A (GCG→GCT hypF ← carbamoyltransferase HypF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,835,6890CA100.0% 113.4 / NA 33A579A (GCG→GCThypFcarbamoyltransferase HypF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/18);  total (15/18)

GCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGTTTCGCCACGGCTGCTTCGCTGCCAAATCGCCACCCGGAA  >  NC_000913/2835553‑2835819
                                                                                                                                        |                                                                                                                                  
gcgcCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGcc                                                                                                                                 >  2:867101/1‑140 (MQ=255)
   ccTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGc                                                                                                                              >  1:625514/1‑140 (MQ=255)
    cTCCAGAGCACAAGCCGCTCCACCTTCATAACTTACCGTGGCTGGCGCACAGCCCAGTGCCGCCGCGACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCa                                                                                                                             <  1:667547/140‑1 (MQ=255)
                    gCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTg                                                                                                             <  1:812001/140‑1 (MQ=255)
                         cccTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGca                                                                                                        <  2:145778/139‑1 (MQ=255)
                         aCCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGca                                                                                                        <  1:326998/140‑1 (MQ=255)
                         aCCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGca                                                                                                        >  2:1013506/1‑140 (MQ=255)
                         aCCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGca                                                                                                        <  1:867101/140‑1 (MQ=255)
                               aTAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTg                                                                                                  >  2:655663/1‑140 (MQ=255)
                                     tAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGttt                                                                                            <  2:102510/140‑1 (MQ=255)
                                     tAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGttt                                                                                            >  1:533185/1‑140 (MQ=255)
                                        cGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag                                                                                         >  1:5389/1‑140 (MQ=255)
                                        cGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCag                                                                                         >  2:812968/1‑140 (MQ=255)
                                         gTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCaga                                                                                        <  1:688955/140‑1 (MQ=255)
                                          tggctggcGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTACACACTTGCTGTTTCagag                                                                                       >  1:526189/1‑140 (MQ=255)
                                           ggctggcGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGt                                                                                      <  1:1013506/140‑1 (MQ=255)
                                           ggctggcGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGt                                                                                      >  1:859526/1‑140 (MQ=255)
                                                     caGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCa                                                                            <  1:313818/140‑1 (MQ=255)
                                                        cccAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTc                                                                         <  1:655663/140‑1 (MQ=255)
                                                                cgccgccACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAGTTCTGCCACTCCGGCACaa                                                                 >  2:951200/1‑140 (MQ=255)
                                                                  ccgccACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCa                                                                            <  1:524476/127‑1 (MQ=255)
                                                                  ccgccACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCCCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCa                                                                            >  2:524476/1‑127 (MQ=255)
                                                                             tCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTg                                                    <  2:979833/140‑1 (MQ=255)
                                                                             tCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTg                                                    <  2:570519/140‑1 (MQ=255)
                                                                             tCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTg                                                    >  2:358151/1‑140 (MQ=255)
                                                                                     aCGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCcagca                                            <  1:425981/140‑1 (MQ=255)
                                                                                        cccACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGt                                         <  1:956206/140‑1 (MQ=255)
                                                                                        cccACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGtt                                        >  1:189635/1‑140 (MQ=255)
                                                                                             acGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGTTTCGc                                    <  2:533185/140‑1 (MQ=255)
                                                                                                                        cACGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGTTTCGCCACGGCTGCTTCGCTGCCAAATCGCCa         <  2:526189/140‑1 (MQ=255)
                                                                                                                          cGCTCAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGTTTCGCCATGGCTGCTTCGCTGCCAAATCGCCAcc       >  1:860139/1‑140 (MQ=255)
                                                                                                                              cAATGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGTTTCGCCACGGCTGCTTCGCTGCCAAATCGCCACCCGGa   <  2:859526/140‑1 (MQ=255)
                                                                                                                               aaTGGCCCGAGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGTTTCGCCACGGCTGCTTCGCTGCCAAATCGCCACCCGGaa  >  2:704990/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                  
GCGCCTCCAGAGCACAAGCCGCTTCACCTTCATAACTTAACGTGGCTGGCGCACAGCCCAGTGCCGCCGCCACTGCATCGAAAAAACGCCCACACGATGACGCCAGCGGCGCGTTAATTCCACGCTCAATGGCCCGCGCCAGCACGCTCCAGTTTTGCTGTTGCACACTTGCTGTTTCAGAGTAATTCTGCCACTCCGGCACAAAGCGCAGGCACTGCGCCAGCAGGTTTCGCCACGGCTGCTTCGCTGCCAAATCGCCACCCGGAA  >  NC_000913/2835553‑2835819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: