Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	364,378	T→C	Y643C (TAC→TGC)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,378	0	T	C	100.0%	225.3 / NA	59	Y643C (TAC→TGC)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/30);  total (29/30)

CTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTT  >  NC_000913/364242‑364514                                                                                                                                         |                                                                                                                                         cTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCAtt                                                                                                                                       >  1:701619/1‑140 (MQ=255)      gTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAAAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTg                                                                                                                                  >  1:259932/1‑140 (MQ=255)         gTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTc                                                                                                                               <  2:799793/140‑1 (MQ=255)            cAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACt                                                                                                                            >  1:1028471/1‑140 (MQ=255)               gCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCg                                                                                                                         >  1:947993/1‑140 (MQ=255)               gCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCg                                                                                                                         <  1:814731/140‑1 (MQ=255)                cAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGa                                                                                                                        >  2:135965/1‑140 (MQ=255)                   ttCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATgg                                                                                                                     >  1:597295/1‑140 (MQ=255)                   ttCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATgg                                                                                                                     >  1:602060/1‑140 (MQ=255)                    tcaatcaaCTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGt                                                                                                                    <  1:135965/140‑1 (MQ=255)                         caaCTGTTTACCTTGTGGAGCGACATCCAAAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGcc                                                                                                               <  2:259932/140‑1 (MQ=255)                             tGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGa                                                                                                           <  2:701619/140‑1 (MQ=255)                                      tgtgGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGaa                                                                                                  >  2:843944/1‑140 (MQ=255)                                       gtgGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAAc                                                                                                 >  1:581139/1‑140 (MQ=255)                                       gtgGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAAc                                                                                                 <  1:695590/140‑1 (MQ=255)                                         ggAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTg                                                                                               >  2:569537/1‑140 (MQ=255)                                                  tCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAActg                                                                                      >  2:953656/1‑140 (MQ=255)                                                      gagGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAActgctgc                                                                                  <  1:690903/140‑1 (MQ=255)                                                         gCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTgg                                                                               >  1:570261/1‑140 (MQ=255)                                                          cACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAActgctgctg                                                                                <  2:904480/138‑1 (MQ=255)                                                          cACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAActgctgctg                                                                                >  1:904480/1‑138 (MQ=255)                                                          cACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGgt                                                                              >  1:857933/1‑140 (MQ=255)                                                           aCTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGgtg                                                                             >  1:208224/1‑140 (MQ=255)                                                             ttCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGtt                                                                           >  1:842437/1‑140 (MQ=255)                                                             ttCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGtt                                                                           >  1:933130/1‑140 (MQ=255)                                                                  cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAActgctg                                                                                   <  1:841724/127‑1 (MQ=255)                                                                  cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAActgctg                                                                                   >  2:841724/1‑127 (MQ=255)                                                                  cGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCt                                                                      >  1:849886/1‑140 (MQ=255)                                                                                  aCCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGAt                                                      <  2:602060/140‑1 (MQ=255)                                                                                      tCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATg                                                     >  2:902577/1‑137 (MQ=255)                                                                                      tCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATg                                                     <  1:902577/137‑1 (MQ=255)                                                                                       ccAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATgcggc                                                 <  1:569537/140‑1 (MQ=255)                                                                                         aGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGt                                               <  2:1027801/140‑1 (MQ=255)                                                                                           cgcCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGc                                             >  2:582940/1‑140 (MQ=255)                                                                                             ccaccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCgg                                           <  2:581139/140‑1 (MQ=255)                                                                                              caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                          <  2:947993/140‑1 (MQ=255)                                                                                              caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                          <  2:857933/140‑1 (MQ=255)                                                                                              caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                          >  2:986430/1‑140 (MQ=255)                                                                                              caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                          <  2:1028471/140‑1 (MQ=255)                                                                                              caccaTCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGt                                          <  1:929265/140‑1 (MQ=255)                                                                                                   tCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCa                                     <  1:582940/140‑1 (MQ=255)                                                                                                   tCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGATTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCa                                     <  1:719542/140‑1 (MQ=255)                                                                                                        tGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagac                                >  2:169291/1‑140 (MQ=255)                                                                                                        tGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagac                                >  1:343107/1‑140 (MQ=255)                                                                                                        tGCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagac                                >  1:916931/1‑140 (MQ=255)                                                                                                         gCAGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagacc                               <  2:343107/140‑1 (MQ=255)                                                                                                           aGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                             <  1:169291/140‑1 (MQ=255)                                                                                                           aGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                             <  2:916931/140‑1 (MQ=255)                                                                                                           aGGAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccag                             <  2:933130/140‑1 (MQ=255)                                                                                                            ggAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccaga                            <  1:843944/140‑1 (MQ=255)                                                                                                             gAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccagac                           >  2:897261/1‑140 (MQ=255)                                                                                                             gAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccagac                           <  1:986430/140‑1 (MQ=255)                                                                                                             gAGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccagac                           >  1:416372/1‑140 (MQ=255)                                                                                                              aGCTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAagaccagacc                          >  2:872093/1‑140 (MQ=255)                                                                                                                cTCGTTATCGCTATGACGGAACAGGCATTCGCTGGTCCCTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGt                        <  2:849886/140‑1 (MQ=255)                                                                                                                  cGTTATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGAGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGtta                      >  1:1021473/1‑139 (MQ=255)                                                                                                                     tATCGCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATa                   <  2:842437/140‑1 (MQ=255)                                                                                                                         gCTATGACGGAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGa               <  2:570261/140‑1 (MQ=255)                                                                                                                                 ggAACAGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGa       <  1:897261/140‑1 (MQ=255)                                                                                                                                      aGGCATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGtt  <  1:953656/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         CTGCGGTAGTTCAGGCAGTTCAATCAACTGTTTACCTTGTGGAGCGACATCCAGAGGCACTTCACCGCTTGCCAGCGGCTTACCATCCAGCGCCACCATCCAGTGCAGGAGCTCGTTATCGCTATGACGGAACAGGTATTCGCTGGTCACTTCGATGGTTTGCCCGGATAAACGGAACTGGAAAAACTGCTGCTGGTGTTTTGCTTCCGTCAGCGCTGGATGCGGCGTGCGGTCGGCAAAGACCAGACCGTTCATACAGAACTGGCGATCGTT  >  NC_000913/364242‑364514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑