Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,546,932	T→A	V125E (GTG→GAG)	gntT →	high‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,546,932	0	T	A	100.0%	283.5 / NA	80	V125E (GTG→GAG)	gntT	high‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (48/32);  total (48/32)

GCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGAT  >  NC_000913/3546797‑3547068                                                                                                                                        |                                                                                                                                         gcgcACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGttt                                                                                                                                      >  1:981464/1‑140 (MQ=255)  cgcACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGACTTTGTGCTGATGCTGTCGCTGGAGTTTa                                                                                                                                     >  2:470237/1‑140 (MQ=255)    cacaACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTAcc                                                                                                                                   >  1:220569/1‑140 (MQ=255)           tATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCgg                                                                                                                            >  1:476104/1‑140 (MQ=255)            aTCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGACTTTGTGCTGATGCTGTCGCTGGAGTTTACCATCGCGGc                                                                                                                           <  1:470237/140‑1 (MQ=255)                    cacGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGa                                                                                                                   >  2:927376/1‑140 (MQ=255)                      cGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAat                                                                                                                 >  1:794961/1‑140 (MQ=255)                           aTTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTcc                                                                                                            >  1:752219/1‑140 (MQ=255)                           aTTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTcc                                                                                                            >  1:397365/1‑140 (MQ=255)                            ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCa                                                                                                           <  2:476104/140‑1 (MQ=255)                            ttGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCa                                                                                                           <  1:560988/140‑1 (MQ=255)                              gCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCAct                                                                                                         >  1:102215/1‑140 (MQ=255)                               ccAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCActg                                                                                                        <  1:926682/140‑1 (MQ=255)                               ccAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCActg                                                                                                        <  2:88477/140‑1 (MQ=255)                                 aaaTTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCActgct                                                                                                      <  1:1016979/140‑1 (MQ=255)                                    tttGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTa                                                                                                   <  1:927376/140‑1 (MQ=255)                                         tAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTg                                                                                              >  2:242037/1‑140 (MQ=255)                                            aaaaCACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGgtg                                                                                           <  1:734847/140‑1 (MQ=255)                                              aaCACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTa                                                                                         <  1:492137/140‑1 (MQ=255)                                                     ccAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATgg                                                                                  >  1:888544/1‑140 (MQ=255)                                                        gTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAAt                                                                                    >  2:859774/1‑135 (MQ=255)                                                        gTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAAt                                                                                    <  1:859774/135‑1 (MQ=255)                                                         tGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcggc                                                                              <  2:1007586/140‑1 (MQ=255)                                                         tGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATggcggc                                                                              >  2:699007/1‑140 (MQ=255)                                                             gggtggtACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                          <  2:523234/139‑1 (MQ=255)                                                             cGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                          <  1:699007/140‑1 (MQ=255)                                                                    aCTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCActgtctg                                                                   <  2:794961/140‑1 (MQ=255)                                                                     cTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCActgtctgt                                                                  >  2:2942/1‑140 (MQ=255)                                                                          cGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                          >  1:540582/1‑127 (MQ=255)                                                                          cGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                          >  1:148808/1‑127 (MQ=255)                                                                          cGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                          <  2:148808/127‑1 (MQ=255)                                                                          cGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCa                                                                          <  2:540582/127‑1 (MQ=255)                                                                          cGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGAccc                                                             >  2:167191/1‑140 (MQ=255)                                                                             ttttACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACg                                                          >  1:120892/1‑140 (MQ=255)                                                                                       ggTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGcc                                                >  2:404871/1‑140 (MQ=255)                                                                                        gTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCa                                               <  2:981464/140‑1 (MQ=255)                                                                                                cTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATcc                                       <  1:404871/140‑1 (MQ=255)                                                                                                 tGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCg                                      <  2:120892/140‑1 (MQ=255)                                                                                                 tGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCg                                      <  2:888544/140‑1 (MQ=255)                                                                                                  gTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCgg                                     >  2:357214/1‑140 (MQ=255)                                                                                                   ttCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCggg                                    >  2:345555/1‑140 (MQ=255)                                                                                                   ttCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCCCATCCggg                                    >  2:411993/1‑140 (MQ=255)                                                                                                        tGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGa                               <  2:752219/140‑1 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGa                               <  2:604827/135‑1 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGa                               >  1:604827/1‑135 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  2:1007442/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  1:445668/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  1:308102/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  2:323024/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  2:880395/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  2:191138/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  2:169408/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  2:160908/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  2:908934/1‑140 (MQ=255)                                                                                                             tGGGCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCg                          >  1:839392/1‑140 (MQ=255)                                                                                                               ggCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGAt                        >  2:686572/1‑140 (MQ=255)                                                                                                               ggCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGAt                        >  1:920024/1‑140 (MQ=255)                                                                                                               ggCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGAt                        >  1:110119/1‑140 (MQ=255)                                                                                                               ggCTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGGCTGTGGCCCACGGCTTCCTGCCACCGCATCCGGGTCAGACTGCGAt                        >  2:720880/1‑140 (MQ=255)                                                                                                                 cTTTGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTg                      <  2:1024438/140‑1 (MQ=255)                                                                                                                   ttGTGCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGcc                    <  1:242037/140‑1 (MQ=255)                                                                                                                    tgtgCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGcca                   >  2:447539/1‑140 (MQ=255)                                                                                                                    tgtgCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGcca                   <  2:130662/140‑1 (MQ=255)                                                                                                                      tgCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGccacc                 <  1:1007442/140‑1 (MQ=255)                                                                                                                       gCTGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGccacca                >  1:247118/1‑140 (MQ=255)                                                                                                                         tGATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCAtt              <  1:751334/140‑1 (MQ=255)                                                                                                                          gATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCAttt             >  2:569034/1‑140 (MQ=255)                                                                                                                          gATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCAttt             >  1:70677/1‑140 (MQ=255)                                                                                                                          gATGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACCGTCTGTGACCCACCGCTTCCTGCCACCGCATCCGGGTCCGACTTCGCTTGCCACCAttt             >  2:171858/1‑140 (MQ=255)                                                                                                                           aTGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCAtttt            >  2:235714/1‑140 (MQ=255)                                                                                                                           aTGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCAtttt            <  1:686572/140‑1 (MQ=255)                                                                                                                           aTGCTGCCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCAtttt            >  1:230115/1‑140 (MQ=255)                                                                                                                              ctgcCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCaa         >  1:816553/1‑140 (MQ=255)                                                                                                                               tgcCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAAt        <  1:169408/140‑1 (MQ=255)                                                                                                                                gcCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATg       >  2:99416/1‑140 (MQ=255)                                                                                                                                gcCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATg       >  1:54546/1‑140 (MQ=255)                                                                                                                                gcCGCTGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATg       <  2:70677/140‑1 (MQ=255)                                                                                                                                     tGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGat  <  1:235714/140‑1 (MQ=255)                                                                                                                                     tGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGat  >  2:944003/1‑140 (MQ=255)                                                                                                                                     tGGAGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGat  <  2:445668/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GCGCACAACGTATCGCCACCACGCTGATTGCCAAATTTGGTAAAAAACACATCCAGTGGGCGGTGGTACTGACCGGTTTTACCGTTGGTTTTGCCCTGTTCTATGAAGTGGGCTTTGTGCTGATGCTGCCGCTGGTGTTTACCATCGCGGCTTCTGCGAATATTCCACTGCTGTATGTTGGTGTACCAATGGCGGCTGCACTGTCTGTGACCCACGGCTTCCTGCCACCGCATCCGGGTCCGACTGCGATTGCCACCATTTTCAATGCCGAT  >  NC_000913/3546797‑3547068

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑