Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,319,016	G→T	D197E (GAC→GAA)	phnJ ←	carbon‑phosphorus lyase core complex subunit PhnJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,319,016	0	G	T	100.0%	152.1 / NA	43	D197E (GAC→GAA)	phnJ	carbon‑phosphorus lyase core complex subunit PhnJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/17);  total (26/17)

CTGCTGAACGGTGAACGGGTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATGTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCA  >  NC_000913/4318899‑4319152                                                                                                                      |                                                                                                                                         ctgctgAACGGTGAACGGGTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAAtt                                                                                                                    >  1:617536/1‑140 (MQ=255)       aacggtgaacggGTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGa                                                                                                              >  1:931990/1‑140 (MQ=255)       aacggtgaacggGTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGa                                                                                                              >  1:425958/1‑140 (MQ=255)            tgaacggGTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGc                                                                                                         >  1:81580/1‑140 (MQ=255)            tgaacggGTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGc                                                                                                         >  1:642585/1‑140 (MQ=255)                 gggTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACgg                                                                                                    <  2:425958/140‑1 (MQ=255)                   gTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGt                                                                                                  <  1:502487/140‑1 (MQ=255)                    tGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTc                                                                                                 >  2:6253/1‑140 (MQ=255)                      aTCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCa                                                                                               >  2:679034/1‑140 (MQ=255)                      aTCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCa                                                                                               >  2:677674/1‑140 (MQ=255)                          tcgAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCAc                                                                                           >  2:190208/1‑140 (MQ=255)                                                   tAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGa                                                                  <  2:642585/140‑1 (MQ=255)                                                    aaaCGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGAt                                                                 <  1:570319/140‑1 (MQ=255)                                                        ggcggcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGc                                                             <  1:442555/140‑1 (MQ=255)                                                          cggcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGt                                                           >  2:880173/1‑140 (MQ=255)                                                          cggcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGt                                                           >  2:622311/1‑140 (MQ=255)                                                          cggcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGt                                                           >  2:1001602/1‑140 (MQ=255)                                                          cggcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGt                                                           >  1:1033223/1‑140 (MQ=255)                                                            gcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGa                                                                  <  2:146540/131‑1 (MQ=255)                                                            gcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGa                                                                  >  1:146540/1‑131 (MQ=255)                                                            gcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAg                                                         >  2:319792/1‑140 (MQ=255)                                                            gcACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAg                                                         >  1:757203/1‑140 (MQ=255)                                                               ccGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAggtg                                                      <  2:617536/140‑1 (MQ=255)                                                                 gCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAggtggt                                                    <  1:679034/140‑1 (MQ=255)                                                                 gCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAggtggt                                                    <  1:6253/140‑1 (MQ=255)                                                                 gCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAggtggt                                                    <  1:319792/140‑1 (MQ=255)                                                                   aTAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGtggtgg                                                  <  2:537590/140‑1 (MQ=255)                                                                       aTGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGat                                              <  2:996996/140‑1 (MQ=255)                                                                          cgcTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATg                                           >  2:748239/1‑140 (MQ=255)                                                                           gcTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGa                                          >  2:65558/1‑140 (MQ=255)                                                                            cTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGAc                                         >  1:512811/1‑140 (MQ=255)                                                                            cTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGAc                                         >  1:380819/1‑140 (MQ=255)                                                                                   cgcCCCGCGCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCg                                  <  1:174449/140‑1 (MQ=255)                                                                                        cgcgCCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGa                             <  1:677674/140‑1 (MQ=255)                                                                                          cgcCGAACAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGata                           <  1:219412/140‑1 (MQ=255)                                                                                                aCAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCa                     >  2:148011/1‑140 (MQ=255)                                                                                                 cAGTTGCAGGGCGGGCATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCAt                    <  2:81580/140‑1 (MQ=255)                                                                                                         gggcgggcATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTc            >  1:590938/1‑140 (MQ=255)                                                                                                            cgggcATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCAcc         >  2:306512/1‑140 (MQ=255)                                                                                                            cgggcATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCAcc         >  2:854280/1‑140 (MQ=255)                                                                                                              ggcATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTg       >  1:688157/1‑140 (MQ=255)                                                                                                              ggcATCATTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGCTTCACCTg       >  1:18805/1‑140 (MQ=255)                                                                                                                   catTTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCa  <  1:880173/140‑1 (MQ=255)                                                                                                                      |                                                                                                                                         CTGCTGAACGGTGAACGGGTGATCGTCGAAATCGAGACTTTCCACGCGGGTAAACGGCGGCACCGCATAGATGCGCTTCTCGCGCCCCGCGCCGAACAGTTGCAGGGCGGGCATCATGTCCATTTTTGGGTTATCGAATTTCGGGATCGGCGACGGGTCCATCACGTAGCGCCCATTTACCTTCACCGGATAGGCGTAGGTGGTGGCGATATGACCGAAGCGGGCGATATCTTCATACAGTTTCACCTGCATCA  >  NC_000913/4318899‑4319152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑