Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,169,682	C→T	Q231Q (CAG→CAA)	ldtC ←	L,D‑transpeptidase YcfS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,169,682	0	C	T	100.0%	208.2 / NA	59	Q231Q (CAG→CAA)	ldtC	L,D‑transpeptidase YcfS
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/24);  total (35/24)

TTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGAT  >  NC_000913/1169549‑1169818                                                                                                                                      |                                                                                                                                         ttGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTa                                                                                                                                    >  2:741905/1‑140 (MQ=255) ttGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTa                                                                                                                                    >  2:927719/1‑140 (MQ=255)    gcagcaGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAag                                                                                                                                 <  2:518005/140‑1 (MQ=255)     cagcagCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAaga                                                                                                                                <  2:375549/140‑1 (MQ=255)     cagcagCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAaga                                                                                                                                <  1:391590/140‑1 (MQ=255)      agcagcTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAagag                                                                                                                               >  1:430555/1‑140 (MQ=255)        cagcTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAgtg                                                                                                                             <  2:495359/140‑1 (MQ=255)           cTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGttt                                                                                                                          >  1:839328/1‑140 (MQ=255)              cGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGa                                                                                                                       <  2:123937/140‑1 (MQ=255)                           aTCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCg                                                                                                          <  2:986578/140‑1 (MQ=255)                                    gACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAAt                                                                                                 <  1:803039/140‑1 (MQ=255)                                    gACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAAt                                                                                                 <  1:981486/140‑1 (MQ=255)                                    gACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAAt                                                                                                 <  2:430555/140‑1 (MQ=255)                                     aCAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATa                                                                                                >  2:500235/1‑140 (MQ=255)                                      cAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATAc                                                                                               >  1:122692/1‑140 (MQ=255)                                         cGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGc                                                                                            <  1:741905/140‑1 (MQ=255)                                         cGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGc                                                                                            <  1:500235/140‑1 (MQ=255)                                          ggCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGcc                                                                                           >  2:98757/1‑140 (MQ=255)                                           gCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCa                                                                                          >  1:795856/1‑140 (MQ=255)                                           gCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCa                                                                                          >  1:165893/1‑140 (MQ=255)                                                 gTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACtt                                                                                    <  2:122692/140‑1 (MQ=255)                                                   aCTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTAc                                                                                  >  1:181994/1‑140 (MQ=255)                                                     ttCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTAccc                                                                                >  1:802502/1‑140 (MQ=255)                                                        aaCCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCa                                                                             <  2:181994/140‑1 (MQ=255)                                                         aCCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCAt                                                                            >  2:58819/1‑140 (MQ=255)                                                            aGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGcc                                                                         >  1:428134/1‑140 (MQ=255)                                                                   cACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCg                                                                  >  1:736073/1‑140 (MQ=255)                                                                        tttGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATc                                                             >  1:122669/1‑140 (MQ=255)                                                                        tttGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATc                                                             >  2:910635/1‑140 (MQ=255)                                                                        tttGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATc                                                             >  1:312284/1‑140 (MQ=255)                                                                                ggCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCg                                                     <  1:258326/140‑1 (MQ=255)                                                                                ggCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCg                                                     >  2:45046/1‑140 (MQ=255)                                                                                  cAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTa                                                   >  2:789051/1‑140 (MQ=255)                                                                                       aCTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATg                                              >  2:171668/1‑140 (MQ=255)                                                                                       aCTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATg                                              <  1:524215/140‑1 (MQ=255)                                                                                                ggAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATa                                     <  2:795856/140‑1 (MQ=255)                                                                                                   gtgtTGATGATATTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAAc                                  >  1:99428/1‑140 (MQ=255)                                                                                                            atatTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATa                         <  1:901982/140‑1 (MQ=255)                                                                                                            atatTCACTTTGG‑GCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAg                        >  1:383990/1‑140 (MQ=255)                                                                                                              atTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACATCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAgg                       >  1:569459/1‑140 (MQ=255)                                                                                                              atTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAgg                       >  1:596357/1‑140 (MQ=255)                                                                                                              atTCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAgg                       >  1:428781/1‑140 (MQ=255)                                                                                                               ttCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgc                      >  1:812451/1‑140 (MQ=255)                                                                                                                tCACTTTGGTGCCTGGGGTGACTTGGCTAAAGGGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGACATAGgcc                     >  2:770221/1‑140 (MQ=255)                                                                                                                tCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgcc                     >  2:675917/1‑140 (MQ=255)                                                                                                                tCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgcc                     >  1:114054/1‑140 (MQ=255)                                                                                                                tCACTTTGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGgcc                     >  2:432892/1‑140 (MQ=255)                                                                                                                    tttGGTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCa                 >  2:975530/1‑140 (MQ=255)                                                                                                                       ggTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGAc              >  1:647303/1‑140 (MQ=255)                                                                                                                        gggCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACg             <  2:383990/138‑1 (MQ=255)                                                                                                                        gTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACg             <  2:428134/140‑1 (MQ=255)                                                                                                                        gTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACg             <  2:802502/140‑1 (MQ=255)                                                                                                                        gTGCCTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACg             <  2:114054/140‑1 (MQ=255)                                                                                                                           ccTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAAt          <  1:98757/140‑1 (MQ=255)                                                                                                                           ccTGGGGTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAAt          >  1:239090/1‑140 (MQ=255)                                                                                                                           ccTGGGGTGACTTGGCTAAAGAGAGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAAt          >  2:206862/1‑140 (MQ=255)                                                                                                                              ggggTGACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGc       >  2:525265/1‑140 (MQ=255)                                                                                                                                   gACTTGGCTAAAGGGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGACATAGGCCGCCAGACGAATCGCatgat  <  1:770221/140‑1 (MQ=255)                                                                                                                                   gACTTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCatgat  <  1:366067/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TTGGCAGCAGCTGCGGATCGTCATCAATCTTCTCTGACAGCGGCTGATGTACTTCAACCAGACGCGCACCGTTTGGTTCGGCAGAGACTTTTATCGGAGTGTTGATGATATTCACTTTGGTGCCTGGGGTGACCTGGCTAAAGAGTGTTTTGATATCGTCATCCCGCAGACGAATACAGCCAGAACTTACCCGCATGCCAATGCCGAAATCGGCGTTCGTACCATGAAGCAAATAAACGCCGCCATAGGCCGCCAGACGAATCGCATGAT  >  NC_000913/1169549‑1169818

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑