Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	637,008	IS5 (+) +4 bp	coding (608‑611/903 nt)	ybdO ←	putative LysR family DNA‑binding transcriptional regulator YbdO

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	637008 =	0 (0.000)	34 (0.870)	27/278	0.4	100%	coding (611/903 nt)	ybdO	putative LysR family DNA‑binding transcriptional regulator YbdO
	?	NC_000913	687851 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 637011	0 (0.000)	31 (0.790)	29/278	0.3	100%	coding (608/903 nt)	ybdO	putative LysR family DNA‑binding transcriptional regulator YbdO
	?	NC_000913	= 689045	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

GTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAA  >  NC_000913/688922‑689183                                                                                                                            |                                                                                                                                           gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGt                                                                                                                            >  1:193238/1‑140 (MQ=37) gTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGt                                                                                                                            <  2:507586/140‑1 (MQ=38)  tCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGtt                                                                                                                           >  1:342511/1‑140 (MQ=38)       cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCt                                                                                                                      <  1:604601/140‑1 (MQ=255)       cGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCt                                                                                                                      <  2:618937/140‑1 (MQ=255)        ggCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACGAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTa                                                                                                                     <  1:277070/140‑1 (MQ=255)            cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATg                                                                                                                 >  1:517045/1‑140 (MQ=255)                    tgAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGata                                                                                                         <  2:193238/140‑1 (MQ=255)                     gAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGatat                                                                                                        <  1:416052/140‑1 (MQ=255)                       aTTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTg                                                                                                      <  2:517045/140‑1 (MQ=255)                                cGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTAt                                                                                             >  1:459487/1‑140 (MQ=255)                                                     tCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTCGCGAGATgcg                                                                        >  1:430251/1‑140 (MQ=255)                                                       atgatgAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGcgcc                                                                      >  2:270776/1‑140 (MQ=255)                                                               cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCAtt                                                              >  1:413123/1‑140 (MQ=255)                                                               cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCAtt                                                              >  1:320344/1‑140 (MQ=255)                                                               cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCAtt                                                              >  1:310011/1‑140 (MQ=255)                                                               cccTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCAtt                                                              >  1:307030/1‑140 (MQ=255)                                                                  tGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATgcg                                                                        <  2:578428/127‑1 (MQ=255)                                                                  tGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATgcg                                                                        >  1:578428/1‑127 (MQ=255)                                                                  tGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCg                                                           <  2:342511/140‑1 (MQ=255)                                                                            cTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAAtt                                                 >  2:415026/1‑140 (MQ=255)                                                                              ccAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCt                                               >  2:418415/1‑140 (MQ=255)                                                                              ccAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCt                                               >  2:541828/1‑140 (MQ=255)                                                                              ccAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCt                                               >  2:62214/1‑140 (MQ=255)                                                                                     aCAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATg                                        <  2:413123/140‑1 (MQ=255)                                                                                      cAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGc                                       >  2:482762/1‑140 (MQ=255)                                                                                              aTCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCtttt                               >  2:625717/1‑140 (MQ=255)                                                                                                   atatCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGc                          >  1:431120/1‑140 (MQ=255)                                                                                                    tatCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCt                         >  2:250305/1‑140 (MQ=255)                                                                                                    tatCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCt                         <  1:202053/140‑1 (MQ=255)                                                                                                        aGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCg                     <  1:415026/140‑1 (MQ=255)                                                                                                          ggACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCg                     >  2:413355/1‑138 (MQ=255)                                                                                                          ggACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCg                     <  1:413355/138‑1 (MQ=255)                                                                                                          ggACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCt                   >  2:643643/1‑140 (MQ=255)                                                                                                           gACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTg                  <  2:480248/140‑1 (MQ=255)                                                                                                             cTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGa                >  2:648100/1‑140 (MQ=255)                                                                                                             cTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGa                >  2:514562/1‑140 (MQ=255)                                                                                                               tGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGAtt              >  1:505713/1‑140 (MQ=255)                                                                                                                      aCCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTgtgg       <  1:418415/140‑1 (MQ=255)                                                                                                                        cttccttAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTgtggtg     >  2:200810/1‑140 (MQ=255)                                                                                                                           ccttAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCaa  <  2:505713/140‑1 (MQ=255)                                                                                                                           ccttAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCaa  >  1:651426/1‑140 (MQ=255)                                                                                                                            |                                                                                                                                           GTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGTAACATTTAGTTTGGCTAAATGTAAAGATATTGCTGTTTTATTGTTTGTTTTTGCGAGATGCGCCGCACCATTCCGAAGCAAAATTCTTAAAATGCACTCTTTTAGTGCTACCGCTGGATTACTGTGGTGCAA  >  NC_000913/688922‑689183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.