Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,579	C→A	A316A (GCC→GCA)	radD →	putative DNA repair helicase RadD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,579	0	C	A	100.0%	127.1 / NA	38	A316A (GCC→GCA)	radD	putative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/19);  total (19/19)

ACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCCCCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCA  >  NC_000913/2281444‑2281713                                                                                                                                        |                                                                                                                                       aCTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGc                                                                                                                                    <  2:153902/140‑1 (MQ=255)       tgccGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCg                                                                                                                              >  2:63948/1‑140 (MQ=255)       tgccGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCg                                                                                                                              >  2:613563/1‑140 (MQ=255)        gccGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGa                                                                                                                             >  1:323806/1‑140 (MQ=255)               aTGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATc                                                                                                                      >  2:334525/1‑140 (MQ=255)                       cTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCt                                                                                                              <  2:265863/140‑1 (MQ=255)                          aTTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTgcg                                                                                                           <  1:334525/140‑1 (MQ=255)                             aCTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGccc                                                                                                        <  2:323806/140‑1 (MQ=255)                               tGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTa                                                                                                      >  1:103602/1‑140 (MQ=255)                               tGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTa                                                                                                      >  2:481954/1‑140 (MQ=255)                                 gCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTa                                                                                                      <  1:309277/138‑1 (MQ=255)                                 gCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTa                                                                                                      >  2:309277/1‑138 (MQ=255)                                    acacCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGaa                                                                                                 >  2:250661/1‑140 (MQ=255)                                    acacCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGaa                                                                                                 >  2:229862/1‑140 (MQ=255)                                    acacCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGaa                                                                                                 >  2:648916/1‑140 (MQ=255)                                    acacCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGaa                                                                                                 >  2:79281/1‑140 (MQ=255)                                    acacCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGaa                                                                                                 <  1:250661/140‑1 (MQ=255)                                         cccGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGt                                                                                            <  1:63948/140‑1 (MQ=255)                                         cccGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGt                                                                                            <  1:613563/140‑1 (MQ=255)                                                  gAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTa                                                                                   >  2:497941/1‑140 (MQ=255)                                                  gAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTa                                                                                   >  2:83664/1‑140 (MQ=255)                                                  gAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTa                                                                                   >  1:580756/1‑140 (MQ=255)                                                              ttAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGt                                                                       <  1:229862/140‑1 (MQ=255)                                                                    gAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGgcg                                                                 >  1:355168/1‑140 (MQ=255)                                                                     aaaaTTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGgcgc                                                                <  2:34342/140‑1 (MQ=255)                                                                      aaaTTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGcgcg                                                               <  1:342680/140‑1 (MQ=255)                                                                      aaaTTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGcgcg                                                               >  2:342680/1‑140 (MQ=255)                                                                      aaaTTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGcgcg                                                               <  2:355168/140‑1 (MQ=255)                                                                      aaaTTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGcgcg                                                               >  1:510520/1‑140 (MQ=255)                                                                                    aGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCt                                                 <  1:497941/140‑1 (MQ=255)                                                                                    aGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCt                                                 <  2:637831/140‑1 (MQ=255)                                                                                         tttCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCggg                                            <  1:481954/140‑1 (MQ=255)                                                                                                       cAACGTCGCGGTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCt                              <  1:648916/140‑1 (MQ=255)                                                                                                                 gTACTGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGa                    <  1:4869/140‑1 (MQ=255)                                                                                                                     tGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTAt                <  2:580756/140‑1 (MQ=255)                                                                                                                     tGACCACCGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTAt                <  2:103602/140‑1 (MQ=255)                                                                                                                          accGGATTTGACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCggg           >  2:64707/1‑140 (MQ=255)                                                                                                                                   gACGCACCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCa  >  2:196775/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                       ACTGCCTGCCGAAGATGCAGCACTGATTACTGGCGACACCCCCGGCGCTGAGCGCGATGTGTTAATTGAAAATTTTAAAGCCCAGCGTTTTCGCTATCTGGTCAACGTCGCGGTACTGACCACCGGATTTGACGCCCCGCACGTCGATCTTATCGCCATTCTGCGCCCTACCGAATCAGTGAGTCTTTACCAACAAATTGTCGGGCGCGGTCTGCGTCTCGCTCCGGGCAAGACTGATTGCTTAATTCTTGATTATGCGGGTAATCCTCA  >  NC_000913/2281444‑2281713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑