Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,664,273	C→A	intergenic (+28/‑117)	suhB → / → yfhR	inositol‑phosphate phosphatase/putative peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,664,273	0	C	A	100.0%	115.8 / NA	34	intergenic (+28/‑117)	suhB/yfhR	inositol‑phosphate phosphatase/putative peptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/20);  total (14/20)

GGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAGGAAAGGTTATGGCACTGCCA  >  NC_000913/2664138‑2664401                                                                                                                                        |                                                                                                                                 ggtCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGccc                                                                                                                              >  1:377049/1‑140 (MQ=255)  gtCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCt                                                                                                                             >  1:395391/1‑140 (MQ=255)             aTGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAgg                                                                                                                  <  2:179118/140‑1 (MQ=255)                cTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGgcgc                                                                                                               <  1:125315/140‑1 (MQ=255)                cTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGgcgc                                                                                                               <  1:565239/140‑1 (MQ=255)                 tGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCt                                                                                                              >  2:112273/1‑140 (MQ=255)                      ggTAACATGGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTcc                                                                                                         <  1:46048/140‑1 (MQ=255)                        tAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGa                                                                                                       <  2:589992/140‑1 (MQ=255)                          aCATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGaaa                                                                                                     <  2:251517/140‑1 (MQ=255)                               gTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACtt                                                                                                >  1:457460/1‑140 (MQ=255)                                    tGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCa                                                                                           <  2:395391/140‑1 (MQ=255)                                             gcgcgTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTAc                                                                                  >  2:367356/1‑140 (MQ=255)                                                cgTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTActtt                                                                               >  2:335372/1‑140 (MQ=255)                                                    gttAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTActttcttt                                                                           >  1:240597/1‑140 (MQ=255)                                                       aaaGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGt                                                                        >  2:341184/1‑140 (MQ=255)                                                         aGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCa                                                                      <  1:341184/140‑1 (MQ=255)                                                             aTGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCActct                                                                  <  2:240597/140‑1 (MQ=255)                                                             aTGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCActct                                                                  >  2:528228/1‑140 (MQ=255)                                                                 tGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCcca                                                              <  1:610807/140‑1 (MQ=255)                                                                                aCGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGAt                                               <  2:457460/140‑1 (MQ=255)                                                                                  gAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTa                                             <  1:651177/140‑1 (MQ=255)                                                                                    gTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAAt                                           <  1:335372/140‑1 (MQ=255)                                                                                         gCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATg                                          <  1:159344/136‑1 (MQ=255)                                                                                         gCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATg                                          >  2:159344/1‑136 (MQ=255)                                                                                                    aaGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTc                           <  1:51671/140‑1 (MQ=255)                                                                                                     aGCGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCg                          <  1:528228/140‑1 (MQ=255)                                                                                                       cGTTAATGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCa                        <  1:203131/140‑1 (MQ=255)                                                                                                           aaTGACTCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAGGa                    <  2:377049/140‑1 (MQ=255)                                                                                                                 tCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAgg                     >  1:528642/1‑133 (MQ=255)                                                                                                                 tCAGGCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAgg                     <  2:528642/133‑1 (MQ=255)                                                                                                                    ggCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAGGAAAGGTTATg           >  1:592915/1‑140 (MQ=255)                                                                                                                    ggCGGGTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAGGAAAGGTTATg           >  2:448893/1‑140 (MQ=255)                                                                                                                        ggTGATATCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAGGAAAGGTTATGGCAc       >  1:630898/1‑140 (MQ=255)                                                                                                                             tatCACTCACCAGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAGGAAAGGTTATGGCACTGCCa  <  1:367356/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                 GGTCATAACTACATGCTGACCGGTAACATCGTTGCTGGTAACCCGCGCGTTGTTAAAGCCATGCTGGCGAACATGCGTGACGAGTTAAGCGACGCTCTGAAGCGTTAATGACTCAGGCGGGTGATATCACTCACCCGCCCTCGCCTTTCAGGCGCTATTCCGAAATACTTCCTCACTGCTTTACTTTCTTTCGTCACTCTCCCACCATCTTTCCCCGATTAATGGATGATAAAGAAGTCGCAAGGAAAGGTTATGGCACTGCCA  >  NC_000913/2664138‑2664401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑