Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	347,387	C→A	G353W (GGG→TGG)	prpR ←	DNA‑binding transcriptional dual regulator PrpR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	347,387	0	C	A	100.0%	101.0 / NA	30	G353W (GGG→TGG)	prpR	DNA‑binding transcriptional dual regulator PrpR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (22/8);  total (22/8)

TGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGT  >  NC_000913/347263‑347523                                                                                                                             |                                                                                                                                         tGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGAc                                                                                                                           >  2:172322/1‑140 (MQ=255)   cAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACct                                                                                                                         >  1:541656/1‑140 (MQ=255)                  cAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCa                                                                                                          >  1:214017/1‑140 (MQ=255)                   aGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAG‑TTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCAcc                                                                                                        >  2:596448/1‑140 (MQ=255)                      cGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCg                                                                                                      <  2:241378/140‑1 (MQ=255)                                 aTCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCggg                                                                                           >  1:500382/1‑140 (MQ=255)                                      ggCGAAAACGTCCTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGc                                                                                      >  2:109291/1‑140 (MQ=255)                                                 ccTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCgg                                                                           >  1:316/1‑140 (MQ=255)                                                   ttGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCa                                                                         <  2:500382/140‑1 (MQ=255)                                                   ttGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCa                                                                         <  2:195785/140‑1 (MQ=255)                                                    tgctgcATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCAt                                                                        <  2:326945/140‑1 (MQ=255)                                                         cATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGc                                                                   >  2:420074/1‑140 (MQ=255)                                                           tatCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCa                                                                 >  2:104158/1‑140 (MQ=255)                                                              cttcttCCAG‑TTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAAtct                                                             <  1:596448/140‑1 (MQ=255)                                                                 cttcCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCa                                                           >  2:230526/1‑140 (MQ=255)                                                                       gATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGa                                                     >  1:203760/1‑140 (MQ=255)                                                                        aTTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGaa                                                    >  2:188148/1‑140 (MQ=255)                                                                           gCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACa                                                 <  1:230526/140‑1 (MQ=255)                                                                                        cTAATGACCCGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTg                                    >  1:321250/1‑140 (MQ=255)                                                                                                ccGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTggg                                  >  2:285916/1‑134 (MQ=255)                                                                                                ccGTACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGAAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTggg                                  <  1:285916/134‑1 (MQ=255)                                                                                                   tACATCTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGa                         >  2:509095/1‑140 (MQ=255)                                                                                                        cTACCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGc                    >  1:239618/1‑140 (MQ=255)                                                                                                          aCCGGAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGccc                  >  1:466403/1‑140 (MQ=255)                                                                                                              gAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGgcg              >  1:60492/1‑140 (MQ=255)                                                                                                              gAACAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGgcg              >  2:269165/1‑140 (MQ=255)                                                                                                               aaCAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGgcgc             <  1:624327/140‑1 (MQ=255)                                                                                                                aCAGGCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGcgcg            >  2:588521/1‑140 (MQ=255)                                                                                                                    gCTGATGCCAGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCt        >  1:558183/1‑140 (MQ=255)                                                                                                                         tGCCAGCCGACGCGGGTGACCTC‑TTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGt  >  1:367757/1‑140 (MQ=255)                                                                                                                             |                                                                                                                                         TGCAGACGCAAAATACTCAGCCGATAAAACAGATCACGGCGAAAACGTCCTTGCTGCATATCTTCTTCCAGATTGCAGTGAGTGGCGCTAATGACCCGTACATCTACCGGAACAGGCTGATGCCCGCCGACGCGGGTGACCTCTTTTTCTTCCAGCACCCGCAGCAGCCGGGTCTGCAAAGGTAGCGGCATTTCGCCAATCTCATCCAGAAACAGCGTACCGCCGTGGGCAATTTCGAACAGCCCGGCGCGACCTCCGCGT  >  NC_000913/347263‑347523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑