Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,025,672 C→A L229I (CTC→ATC)  ubiD → 3‑octaprenyl‑4‑hydroxybenzoate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,025,6720CA95.0% 131.6 / 1.0 40L229I (CTC→ATC) ubiD3‑octaprenyl‑4‑hydroxybenzoate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (16/22);  total (17/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.69e-01

TTGGTAAAAACAAACTGATTATGCGCTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTCTCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGT  >  NC_000913/4025538‑4025802
                                                                                                                                      |                                                                                                                                  
ttGGTAAAAACAAACTGATTATGCGCTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGt                                                                                                                               <  2:598758/140‑1 (MQ=255)
 tGGTAAAAACAAACTGATTATGCGCTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCGGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTg                                                                                                                              <  1:120075/140‑1 (MQ=255)
              cTGATTATGCGCTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGt                                                                                                                 >  1:111805/1‑140 (MQ=255)
                    aTGCGCTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCGGGGCGAACGTTCCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGa                                                                                                           <  1:323874/140‑1 (MQ=255)
                    aTGCGCTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGa                                                                                                           >  1:242775/1‑140 (MQ=255)
                      gcgcTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATa                                                                                                         <  2:395401/140‑1 (MQ=255)
                      gcgcTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATa                                                                                                         <  2:137196/140‑1 (MQ=255)
                         ctggctgTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGc                                                                                                      >  2:361331/1‑140 (MQ=255)
                           ggctgTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCtt                                                                                                    >  1:76134/1‑140 (MQ=255)
                            gctgTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACTTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCttt                                                                                                   <  2:123305/140‑1 (MQ=255)
                                      cgcgGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGc                                                                                         >  2:232821/1‑140 (MQ=255)
                                      cgcgGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGCGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGGATGc                                                                                         >  2:397075/1‑140 (MQ=255)
                                              cgcgcTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTCCAGAGTATGCGTTTGCCg                                                                                 >  2:425926/1‑140 (MQ=255)
                                                     gATTATCAGGGGGGGGGGGCGGGCCGCCCGGGCGAACGTTTCCCGGTTTCTGTGGCGTTGGGCGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCt                                                                          <  2:192927/140‑1 (MQ=255)
                                                          tCAGGGGTGGTGTGCGGCGCATCGGGGCGAACGTTTCCCGGTTTCTGTGGCGCGGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTg                                                                     <  2:102801/140‑1 (MQ=255)
                                                              gAGTGGTGTGCGGCCCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCAc                                                                 <  2:130611/140‑1 (MQ=255)
                                                               aGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCAcc                                                                >  2:619480/1‑140 (MQ=255)
                                                                   gtgtGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGa                                                            <  1:482463/140‑1 (MQ=255)
                                                                    tgcgcggcgCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGAc                                                           >  2:483302/4‑140 (MQ=255)
                                                                      tgCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCAc                                                                 >  1:447182/1‑132 (MQ=255)
                                                                      tgCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCAc                                                                 <  2:447182/132‑1 (MQ=255)
                                                                        cggcgCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGaa                                                       >  2:444297/1‑140 (MQ=255)
                                                                         ggcgCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTCTCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAg                                                      >  1:563909/1‑140 (MQ=255)
                                                                            gcATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAgtgg                                                   <  1:15184/140‑1 (MQ=255)
                                                                              ttgcGGGCGACGTTTTCCCGGTTTTTGGGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAgtggtg                                                 <  2:279682/137‑1 (MQ=255)
                                                                              aTCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTCTCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAgtggtg                                                 <  2:563909/140‑1 (MQ=255)
                                                                              aTCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAgtggtg                                                 <  1:444297/140‑1 (MQ=255)
                                                                                   ggggatggTTTCCCGGTTTCGGTGCCGTGGGGTCCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAgtg                                            <  1:556218/133‑1 (MQ=255)
                                                                                    gCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAgtgt                                           <  2:245157/140‑1 (MQ=255)
                                                                                     cGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAg                                              <  2:249224/136‑1 (MQ=255)
                                                                                     cGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAg                                              >  1:249224/1‑136 (MQ=255)
                                                                                     cGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTa                                          >  1:424236/1‑140 (MQ=255)
                                                                                            ttCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCaa                                   <  1:619480/140‑1 (MQ=255)
                                                                                            ttCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCaa                                   <  1:308404/140‑1 (MQ=255)
                                                                                                           gcggggggCGCCGACCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGcc                    <  2:407813/135‑1 (MQ=255)
                                                                                                              gggggTGCCGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGc                 <  1:42804/138‑1 (MQ=255)
                                                                                                                     ccGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCg          <  1:232821/140‑1 (MQ=255)
                                                                                                                      cGATCCCGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCgg         >  1:256633/1‑140 (MQ=255)
                                                                                                                           ccGCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGCGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGAtt    >  2:3435/1‑140 (MQ=255)
                                                                                                                             gCCACGATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATtgt  >  2:384082/1‑140 (MQ=255)
                                                                                                                                  gATTATCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCGGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATtg   >  2:243015/1‑134 (MQ=255)
                                                                                                                                  gATTATCGGTGCAGTCACTCCCGTTCCGAATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATtg   <  1:243015/134‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                  
TTGGTAAAAACAAACTGATTATGCGCTGGCTGTCGCATCGCGGCGGCGCGCTGGATTATCAGGAGTGGTGTGCGGCGCATCCGGGCGAACGTTTCCCGGTTTCTGTGGCGCTGGGTGCCGATCCCGCCACGATTCTCGGTGCAGTCACTCCCGTTCCGGATACGCTTTCAGAGTATGCGTTTGCCGGATTGCTACGTGGCACCAAGACCGAAGTGGTGAAGTGTATCTCCAATGATCTTGAAGTGCCCGCCAGTGCGGAGATTGT  >  NC_000913/4025538‑4025802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑