Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,111,7470GT26.8% 105.2 / 15.2 41N156K (AAC→AAA) yiiQDUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (28/2);  new base T (0/11);  total (28/13)
Fisher's exact test for biased strand distribution p-value = 2.47e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111612‑4111885
                                                                                                                                        |                                                                                                                                          
ttGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGtttt                                                                                                                                        >  2:958155/1‑140 (MQ=255)
     ttttATTAAACCCTTCAAGCGATTCAGATAGCGCCAGCTAATTCGTTTCAACAGCGAAGGCCAGCCCCTTTCCGCCGTTGCCCGCA‑ACAACATAACGCAGTCCACCTTCTGTTTCGGTTTAATATCTTTTTTTTTCcccc                                                                                                                                   <  2:245212/139‑1 (MQ=255)
      tttATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCccg                                                                                                                                  >  1:245212/1‑140 (MQ=255)
                    cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGAc                                                                                                                    >  1:198752/1‑140 (MQ=255)
                     aaGCGATTCAGATAGCGCAACTTAAATGGGTCAAACAGCGAGGGCCACCCCTTTTCCGCGGTTGCCCCCA‑AAAACATAACGCGGTGCCCCTTCTGTCTGGGTGAAATAACTTTTTTTTTTCCCCGCCGTTAGTAGCGACt                                                                                                                   <  2:679401/140‑1 (MQ=255)
                     aaGCGATTAAGATGGCGCCAGCTTAATCGGTTCAACAGCGAGGGTCACCCCTTTTTCGCCGTTGCCCGCG‑CAAACATAACGCAGTGCCCCTTCTGGCTCGGTTTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACt                                                                                                                   <  2:357007/140‑1 (MQ=255)
                         gATTCAGATGGCGCCAGTTTAATCGGTCCAACAGCGAAGGTCCCCCCTTTTCCGCCGTTGCCCGCA‑AAAACATAACGCAGTGCCCCTTCTGTCTCGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAg                                                                                                               <  2:874331/140‑1 (MQ=255)
                                aTAGCGCCAGCTTAATGGGTTCAACAGCGAAGGTCACCCCCTTTTCGCCGTTGCCCGCG‑ACAACATAACGCAGTCCCCCTTCTGTCTCGGTGTAATAACGTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTc                                                                                                        <  2:167165/140‑1 (MQ=255)
                                   gcgcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTgg                                                                                                     >  1:680267/1‑140 (MQ=255)
                                    cgcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGc                                                                                                    >  2:985121/1‑140 (MQ=255)
                                     gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCt                                                                                                   >  2:1026184/1‑140 (MQ=255)
                                                     cAACAGCAAAGGCCACCCCTTTTTCGCCGTTGCCCGCG‑ACAACATAACGCAGTCCCCCTTCTGTCTGGGTTTAATACCTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAtt                                                                                   <  2:198752/140‑1 (MQ=255)
                                                             agggTCACCCTTTTTCCGCCGTTGCCCGAG‑AAAAAAAACCGTTGTCCCCTTTCTTTCGCGGTTTAATAGTGTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:473766/138‑1 (MQ=255)
                                                             aaGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           >  1:359748/1‑140 (MQ=255)
                                                             aaGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCG‑CGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTg                                                                          >  1:1072585/1‑140 (MQ=255)
                                                             aaGGCCACCCCTTTTTCGCCGTTGTCCGCA‑AAAACATACCGCAGTGCCCCTTCTGTCTCGGTGTAATATCTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                           <  2:680267/140‑1 (MQ=255)
                                                                ggtctccccTTTTCGCCCTTTGTCGCGTACAATGTACCGCTGGGCCCTTTTTTTCTCGGTTTACTCTGGTTTTTTTTGCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa                                                                         <  2:729615/135‑1 (MQ=255)
                                                                gTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAg                                                                        >  2:1167981/1‑140 (MQ=255)
                                                                gTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAg                                                                        >  1:274910/1‑140 (MQ=255)
                                                                     ccccTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGc                                                                   >  1:217491/1‑140 (MQ=255)
                                                                     ccccTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGc                                                                   >  2:100498/1‑140 (MQ=255)
                                                                          tttCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                              >  2:191257/1‑140 (MQ=255)
                                                                                 tttGTCCGCA‑AAAACATACCGCAGGGCCCCTTCTGTCTCGGTGTAATAGCGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  1:7884/139‑1 (MQ=255)
                                                                                 tttGCCCGCG‑ACAAAATAACGCAGTGCCCTTTTTGTCTGGGTGTAATAGCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  1:943224/139‑1 (MQ=255)
                                                                                 tttGCCCGCA‑ACAACATACCGCAGTGCCCTTTCTGTCGGGGTTTAATAGCGTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  2:231812/139‑1 (MQ=255)
                                                                                 gTTGCCCGCA‑AAAACATACCGCAGTCCCCCTTCTGTCTCGGTTTAATAATTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                       <  2:837661/140‑1 (MQ=255)
                                                                                      ccGCG‑ACAACATAACGCAGTGCCCCTTCTGTCTGGGTGTAATAACTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATg                                                  <  1:839029/140‑1 (MQ=255)
                                                                                       cgcg‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGt                                                 >  2:16780/1‑140 (MQ=255)
                                                                                             caacaTAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTcc                                            >  1:407157/1‑140 (MQ=255)
                                                                                                        aGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgc                                 >  1:387142/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:422764/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:213632/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:170150/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:62439/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  1:1165484/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:136219/1‑140 (MQ=255)
                                                                                                          tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                               >  2:914252/1‑140 (MQ=255)
                                                                                                                ttCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCgg                         >  2:133077/1‑140 (MQ=255)
                                                                                                                    gTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                     >  2:79843/1‑140 (MQ=255)
                                                                                                                           tgtAATACCTTTTTTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              <  2:387142/140‑1 (MQ=255)
                                                                                                                           tgtAATACCTTTTTTTTTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              <  1:958155/140‑1 (MQ=255)
                                                                                                                               atactgtttttttTCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc          <  2:917820/135‑1 (MQ=255)
                                                                                                                                 agttttttttttCCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcccc        <  1:1042050/136‑1 (MQ=255)
                                                                                                                                   cGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              >  2:518530/1‑132 (MQ=255)
                                                                                                                                   cGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct              <  1:518530/132‑1 (MQ=255)
                                                                                                                                   cGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      >  1:917820/1‑140 (MQ=255)
                                                                                                                                   cGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg      >  1:875247/1‑140 (MQ=255)
                                                                                                                                     tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:530979/1‑140 (MQ=255)
                                                                                                                                     tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  1:433224/1‑140 (MQ=255)
                                                                                                                                     tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  2:682985/1‑140 (MQ=255)
                                                                                                                                     tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  1:130175/1‑140 (MQ=255)
                                                                                                                                     tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  1:782117/1‑140 (MQ=255)
                                                                                                                                     tttgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa    >  1:500312/1‑140 (MQ=255)
                                                                                                                                       tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  2:1008399/1‑140 (MQ=255)
                                                                                                                                       tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  <  2:782117/140‑1 (MQ=255)
                                                                                                                                       tgtttTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg  >  1:536426/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                          
TTGTCATTTATTCAAACCTTCAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTTCGCCGTTGTCCGCG‑ACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCCCCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATG  >  NC_000913/4111612‑4111885

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑