Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	138,373	C→A	R431S (CGT→AGT)	cueO →	cuprous oxidase/multicopper oxidase with role in copper homeostasis

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	138,373	0	C	A	100.0%	260.8 / NA	75	R431S (CGT→AGT)	cueO	cuprous oxidase/multicopper oxidase with role in copper homeostasis
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/43);  total (32/43)

TGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAG  >  NC_000913/138236‑138508                                                                                                                                          |                                                                                                                                        tgGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGt                                                                                                                                       >  1:505247/1‑140 (MQ=255)  gggCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGtt                                                                                                                                      <  1:957735/140‑1 (MQ=255)      cATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGt                                                                                                                                  <  1:746472/140‑1 (MQ=255)      cATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGt                                                                                                                                  <  1:344202/140‑1 (MQ=255)      aatatGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGt                                                                                                                                  <  1:400130/139‑1 (MQ=255)       atatGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGtt                                                                                                                                 >  1:32407/1‑140 (MQ=255)        tatGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTa                                                                                                                                <  2:400923/140‑1 (MQ=255)        tatGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTa                                                                                                                                <  2:1198015/140‑1 (MQ=255)           ggggCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTAtct                                                                                                                             <  2:930887/140‑1 (MQ=255)             ggCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTTGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGGTATttct                                                                                                                           >  1:1086105/1‑140 (MQ=255)               cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGGAATACGAAAGTTGGGTTATCTCTgg                                                                                                                         >  2:1153324/1‑140 (MQ=255)               cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTgg                                                                                                                         >  2:531153/1‑140 (MQ=255)               cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTgg                                                                                                                         >  2:1143875/1‑140 (MQ=255)               cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTgg                                                                                                                         >  2:650505/1‑140 (MQ=255)               cACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAAAAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATctcttg                                                                                                                         >  1:1008501/1‑138 (MQ=255)                 cGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCg                                                                                                                       <  1:1143875/140‑1 (MQ=255)                 cGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCg                                                                                                                       <  1:382226/140‑1 (MQ=255)                 cGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCg                                                                                                                       <  1:418646/140‑1 (MQ=255)                  ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGt                                                                                                                      >  2:135658/1‑140 (MQ=255)                  ggCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGt                                                                                                                      >  2:362041/1‑140 (MQ=255)                  ggCAATATGAATCATATGAACCACGGCGGGAAGGTTGATTTCCACCATGCCAAAAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGGTTGCGGCGGCGGAAGGGCCATACGAAAGGTTGGATATTTCTGGCGt                                                                                                                      >  2:5200/1‑140 (MQ=255)                    cAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTgg                                                                                                                    >  2:1102935/1‑140 (MQ=255)                       tatGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCg                                                                                                                 >  1:7819/1‑140 (MQ=255)                       tatGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCg                                                                                                                 >  1:131914/1‑140 (MQ=255)                          gAATCATATGAACCCCGGCGGGAAGTTCTATTTCCACCATGCCAACAAAATCAACGGTAAGGTGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACa                                                                                                              <  1:213880/140‑1 (MQ=255)                            aTCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACatg                                                                                                            >  2:928759/1‑140 (MQ=255)                                tatGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGAtgc                                                                                                        >  1:58102/1‑140 (MQ=255)                                 atGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGAtgct                                                                                                       >  1:5223/1‑140 (MQ=255)                                 atGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGAtgct                                                                                                       >  1:66145/1‑140 (MQ=255)                                 atGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGAtgct                                                                                                       >  1:13355/1‑140 (MQ=255)                                     aCCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCAt                                                                                                   <  1:362041/140‑1 (MQ=255)                                     aCCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCAt                                                                                                   <  2:32407/140‑1 (MQ=255)                                     aCCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGAGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCAt                                                                                                   >  2:943086/1‑140 (MQ=255)                                      ccACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATc                                                                                                  <  2:632813/140‑1 (MQ=255)                                         cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGTAAGGGCGATACGAAAGATGGGGTATCCCTGGCGAGGGGCACATGATGCTGCAGCCGt                                                                                               >  2:1095066/1‑140 (MQ=255)                                         cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGt                                                                                               >  1:1166224/1‑140 (MQ=255)                                         cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGt                                                                                               >  1:1078689/1‑140 (MQ=255)                                         cggcggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGGTATCTCTGGCGTGGGCGACATGATGGTTCATCCGt                                                                                               >  2:659504/1‑140 (MQ=255)                                            cggGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTcc                                                                                            <  2:505247/140‑1 (MQ=255)                                                  gTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATcc                                                                                      <  2:1166224/140‑1 (MQ=255)                                                   ttCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCa                                                                                     <  1:531153/140‑1 (MQ=255)                                                         ttCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGGCATGATGCTGCATCCGTTCCATATCCACGGCAc                                                                               >  2:239504/1‑140 (MQ=255)                                                          tCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCTTCCGTTCCATATCCACGGCACg                                                                              <  1:152121/140‑1 (MQ=255)                                                          tCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                              <  1:813703/140‑1 (MQ=255)                                                          tCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                              <  1:1144396/140‑1 (MQ=255)                                                          tCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACg                                                                              <  2:1078689/140‑1 (MQ=255)                                                           ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGTAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGc                                                                             <  1:1153324/140‑1 (MQ=255)                                                           ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGc                                                                             <  1:659504/140‑1 (MQ=255)                                                           ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGc                                                                             <  2:637525/140‑1 (MQ=255)                                                           ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGc                                                                             <  2:518113/140‑1 (MQ=255)                                                           ccaccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGc                                                                             <  1:650505/140‑1 (MQ=255)                                                             accaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAg                                                                           >  1:916312/1‑140 (MQ=255)                                                              ccaTGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGt                                                                          <  2:131914/140‑1 (MQ=255)                                                                 tGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTcc                                                                       >  2:74230/1‑140 (MQ=255)                                                                  gCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCg                                                                      >  2:1119089/1‑140 (MQ=255)                                                                  gCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCg                                                                      >  2:77573/1‑140 (MQ=255)                                                                          aaTCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGt                                                              >  1:815168/1‑140 (MQ=255)                                                                              aaCGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGa                                                          >  2:255635/1‑140 (MQ=255)                                                                                  gTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAAt                                                      <  2:372867/140‑1 (MQ=255)                                                                                  gTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAAt                                                      >  1:1174479/1‑140 (MQ=255)                                                                                    cAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATgg                                                    <  2:1174479/140‑1 (MQ=255)                                                                                     aGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGc                                                   >  1:771829/1‑140 (MQ=255)                                                                                                 tGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCg                                       >  1:130838/1‑140 (MQ=255)                                                                                                     cAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTc                                   >  2:969759/1‑140 (MQ=255)                                                                                                        gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATc                                >  1:1009277/1‑140 (MQ=255)                                                                                                        gCCGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATc                                >  1:609881/1‑140 (MQ=255)                                                                                                          cGATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgc                              <  1:77573/140‑1 (MQ=255)                                                                                                           gATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcg                             <  2:815168/140‑1 (MQ=255)                                                                                                           gATGTTTGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcg                             <  1:928202/140‑1 (MQ=255)                                                                                                           gATGTTTGCGGAGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATcgcg                             <  1:943086/140‑1 (MQ=255)                                                                                                                ttGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                        <  1:1144500/140‑1 (MQ=255)                                                                                                                ttGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                        <  1:961845/140‑1 (MQ=255)                                                                                                                ttGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGc                        <  2:66145/140‑1 (MQ=255)                                                                                                                 tGCGGCGGCGAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCt                       <  1:942476/140‑1 (MQ=255)                                                                                                                  gcggcggcgAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTg                      >  1:1145158/1‑140 (MQ=255)                                                                                                                        gcgAAAGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGa                <  1:135658/140‑1 (MQ=255)                                                                                                                           aaaGGGCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATAc             <  2:1009277/140‑1 (MQ=255)                                                                                                                              gggCAATAGGAACGCTGGGTTACCTCTGGCGTGGGCCAAATGATGCTGAATCGCTTCGATTTCCACGGCACGCCGTCCGGTATCTTGTCAGAAAATGGCAAGCCACCAGCGGCTCGTCCCGCGGCCTGGAAAGATACCGt          <  2:701824/140‑1 (MQ=255)                                                                                                                               ggCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGtt         <  2:422907/140‑1 (MQ=255)                                                                                                                                gCAATACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTa        <  1:171928/140‑1 (MQ=255)                                                                                                                                   aTACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAgg     >  2:476245/1‑140 (MQ=255)                                                                                                                                   aTACGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAgg     >  1:1060635/1‑140 (MQ=255)                                                                                                                                     aCGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTa   <  1:1095066/140‑1 (MQ=255)                                                                                                                                      ggAAAGTTGGGTTGTCTCGGGCGTGGGCGACATGATGCTGCATCCGTTACATATCCCCGGCACGCAGTTCGGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAg  <  2:1128362/139‑1 (MQ=255)                                                                                                                                      cGAAAGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAg  <  2:1145158/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TGGGCCATATGGGGCACGGCAATATGAATCATATGAACCACGGCGGGAAGTTCGATTTCCACCATGCCAACAAAATCAACGGTCAGGCGTTTGATATGAACAAGCCGATGTTTGCGGCGGCGAAAGGGCAATACGAACGTTGGGTTATCTCTGGCGTGGGCGACATGATGCTGCATCCGTTCCATATCCACGGCACGCAGTTCCGTATCTTGTCAGAAAATGGCAAACCGCCAGCGGCTCATCGCGCGGGCTGGAAAGATACCGTTAAGGTAG  >  NC_000913/138236‑138508

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑