Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,355,287	G→T	R341R (CGG→CGT)	glpB →	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,355,287	0	G	T	100.0%	171.3 / NA	53	R341R (CGG→CGT)	glpB	anaerobic glycerol‑3‑phosphate dehydrogenase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (24/29);  total (24/29)

ATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGC  >  NC_000913/2355151‑2355422                                                                                                                                         |                                                                                                                                        aTCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGt                                                                                                                                      <  2:333954/140‑1 (MQ=255)    aCGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGaa                                                                                                                                   <  1:1083361/140‑1 (MQ=255)    aCGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGaa                                                                                                                                   <  1:770511/140‑1 (MQ=255)    aCGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGaa                                                                                                                                   <  1:1163702/140‑1 (MQ=255)    aCGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGaa                                                                                                                                   <  1:1200839/140‑1 (MQ=255)          atatTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGtat                                                                                                                             >  1:636550/1‑140 (MQ=255)              tCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTTGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCCCCACGCGAGGTGAATGGTATAAgg                                                                                                                         >  2:946655/1‑140 (MQ=255)               ccGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAggg                                                                                                                        <  1:498960/140‑1 (MQ=255)                  cTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGgaga                                                                                                                     >  2:1049274/1‑140 (MQ=255)                    aCGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGAtt                                                                                                                   >  2:504156/1‑140 (MQ=255)                       tCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGAttttt                                                                                                                >  1:483693/1‑140 (MQ=255)                          aCGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTg                                                                                                             >  1:626954/1‑140 (MQ=255)                                cgcgGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGcgccgc                                                                                                       >  2:1156738/1‑140 (MQ=255)                                cgcgGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGcgccgc                                                                                                       >  2:236062/1‑140 (MQ=255)                                 gcgGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCa                                                                                                      >  2:1160119/1‑140 (MQ=255)                                               cGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGt                                                                                        >  2:524956/1‑140 (MQ=255)                                               cGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGt                                                                                        <  1:1083736/140‑1 (MQ=255)                                               cGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGt                                                                                        >  2:1083736/1‑140 (MQ=255)                                               cGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGt                                                                                        <  1:765320/140‑1 (MQ=255)                                                         tttAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAAc                                                                              <  2:626954/140‑1 (MQ=255)                                                           tAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCa                                                                            <  1:946655/140‑1 (MQ=255)                                                            aGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCAc                                                                           >  1:971658/1‑140 (MQ=255)                                                            aGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCAc                                                                           <  1:1125906/140‑1 (MQ=255)                                                                 cGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACtgatg                                                                      >  1:688751/1‑140 (MQ=255)                                                                   gACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATgag                                                                    >  2:1086792/1‑140 (MQ=255)                                                                   gACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATgag                                                                    >  2:870754/1‑140 (MQ=255)                                                                        gTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCt                                                               >  1:628334/1‑140 (MQ=255)                                                                            cGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGc                                                           <  1:81564/140‑1 (MQ=255)                                                                            cGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGc                                                           <  2:483693/140‑1 (MQ=255)                                                                            cGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGc                                                           <  1:1049274/140‑1 (MQ=255)                                                                            cGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGc                                                           <  2:859403/140‑1 (MQ=255)                                                                                aCGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGt                                                       >  2:473327/1‑140 (MQ=255)                                                                                 cGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTc                                                      <  1:757826/140‑1 (MQ=255)                                                                                 cGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTc                                                      <  1:250663/140‑1 (MQ=255)                                                                                           ttCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAggg                                            >  1:1188463/1‑140 (MQ=255)                                                                                            tCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGc                                           >  2:1122042/1‑140 (MQ=255)                                                                                            tCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACAGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGc                                           >  1:950053/1‑140 (MQ=255)                                                                                               agagCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCaaa                                        <  1:473327/140‑1 (MQ=255)                                                                                                gagCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAAc                                       <  1:236062/140‑1 (MQ=255)                                                                                                gagCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAAc                                       <  1:1156738/140‑1 (MQ=255)                                                                                                                    tgtgCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAACAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCa                   <  1:1160119/140‑1 (MQ=255)                                                                                                                     gtgCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAt                  <  2:688751/140‑1 (MQ=255)                                                                                                                     gtgCTACAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAt                  <  1:504156/140‑1 (MQ=255)                                                                                                                          aCAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAtcggtt             <  1:870754/140‑1 (MQ=255)                                                                                                                          aCAAACCGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAtcggtt             <  2:1188463/140‑1 (MQ=255)                                                                                                                               ccGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTg        <  1:524956/140‑1 (MQ=255)                                                                                                                                cGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAATCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAt                  >  1:992257/1‑129 (MQ=255)                                                                                                                                cGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAATCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAt                  <  2:992257/129‑1 (MQ=255)                                                                                                                                cGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGc       >  1:855312/1‑140 (MQ=255)                                                                                                                                cGCCACGCGTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGc       >  1:1163558/1‑140 (MQ=255)                                                                                                                                  ccACGCGTGATGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAt                  >  2:326488/1‑127 (MQ=255)                                                                                                                                  ccACGCGTGATGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCAt                  <  1:326488/127‑1 (MQ=255)                                                                                                                                     cgcgTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGc  >  2:429093/1‑140 (MQ=255)                                                                                                                                     cgcgTGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACAGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGc  <  2:950053/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        ATCACGCCGATATTCCGCTACGTCCACGTTTCGCGGTTCTCGCCAGCGGCAGTTTCTTTAGTGGCGGACTGGTAGCGGAACGTAACGGCATTCGAGAGCCGATTCTCGGCCTTGATGTGCTACAAACCGCCACGCGGGGTGAATGGTATAAGGGAGATTTTTTTGCGCCGCAACCGTGGCAGCAGTTCGGTGTAACCACTGATGAGACGCTACGCCCGTCACAGGCAGGGCAAACCATTGAAAACCTGTTTGCCATCGGTTCGGTGCTGGGC  >  NC_000913/2355151‑2355422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑