Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,879,713	C→A	R136L (CGC→CTC)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,879,713	0	C	A	98.5%	224.5 / ‑3.2	66	R136L (CGC→CTC)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (36/29);  total (36/30)
Fisher's exact test for biased strand distribution p-value = 4.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

CATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGC  >  NC_000913/3879585‑3879849                                                                                                                                 |                                                                                                                                         caTTGGTGAAGTTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAAcc                                                                                                                               <  2:883520/140‑1 (MQ=255)    tGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGc                                                                                                                            >  1:831630/1‑140 (MQ=255)            gTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGttt                                                                                                                    >  2:878255/1‑140 (MQ=255)            gTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGttt                                                                                                                    >  1:115147/1‑140 (MQ=255)             tttCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGtttt                                                                                                                   >  1:94701/1‑140 (MQ=255)              ttCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGttttt                                                                                                                  >  2:51762/1‑140 (MQ=255)                cGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGc                                                                                                                >  1:840355/1‑140 (MQ=255)                cGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGc                                                                                                                >  1:385374/1‑140 (MQ=255)                cGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGc                                                                                                                >  1:1201967/1‑140 (MQ=255)                   ggCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGAc                                                                                                             <  1:739619/140‑1 (MQ=255)                   ggCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGAc                                                                                                             <  1:164860/140‑1 (MQ=255)                   ggCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGAc                                                                                                             <  1:287698/140‑1 (MQ=255)                    gCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACa                                                                                                            <  2:831630/140‑1 (MQ=255)                      tGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAgg                                                                                                          >  1:778259/1‑140 (MQ=255)                      tGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAgg                                                                                                          <  2:385374/140‑1 (MQ=255)                      tGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAgg                                                                                                          >  1:281401/1‑140 (MQ=255)                      tGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAgg                                                                                                          <  2:840355/140‑1 (MQ=255)                      ggggCCAGAAACGCGCCATGGTGCCGTTTTTTCCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAgg                                                                                                          <  1:941159/139‑1 (MQ=255)                        ggtcAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGc                                                                                                        >  1:582955/4‑140 (MQ=255)                        ggCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGc                                                                                                        >  1:328735/1‑140 (MQ=255)                              aaaCGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTtac                                                                                                  >  2:1023673/1‑140 (MQ=255)                                   cACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCg                                                                                             >  1:199701/1‑140 (MQ=255)                                   cACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCg                                                                                             >  2:1181426/1‑140 (MQ=255)                                        tGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGa                                                                                            <  2:537793/136‑1 (MQ=255)                                        tGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGa                                                                                            >  1:537793/1‑136 (MQ=255)                                        tGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAaca                                                                                        >  2:865292/1‑140 (MQ=255)                                              cgtttttttCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGa                                                                                            <  2:11931/127‑1 (MQ=255)                                              cGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGa                                                                                            >  1:11931/1‑130 (MQ=255)                                                  tttttCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGt                                                                              >  1:1169529/1‑140 (MQ=255)                                                     ttCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGCGTTCGTAGATCTGACGGTGAATTTTAACCTCGAGCTGGATAACCAGCTACAGTTTTTGCGACAGGGCGTTTACTACAGAAAACCCACCGCCGTGCa                                                                           >  2:867594/1‑140 (MQ=255)                                                     tccAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCa                                                                           <  2:115147/138‑1 (MQ=255)                                                          tctcGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGAccg                                                                      <  1:1181426/140‑1 (MQ=255)                                                               ccGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGa                                                                 <  1:560259/140‑1 (MQ=255)                                                               ccGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGa                                                                 <  2:1169529/140‑1 (MQ=255)                                                               ccGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGa                                                                 <  1:213992/140‑1 (MQ=255)                                                               ccGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGa                                                                 <  1:1023673/140‑1 (MQ=255)                                                               ccGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGa                                                                 <  1:51762/140‑1 (MQ=255)                                                               ccGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGa                                                                 <  2:852223/140‑1 (MQ=255)                                                                 ggTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGaca                                                               <  1:865292/140‑1 (MQ=255)                                                                      ccGCCAGCGGGGCATGCGGTACAACGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCCGCTCAAGTTTTCGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTta                                                          <  2:672311/140‑1 (MQ=255)                                                                            gCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAg                                                    >  2:1059775/1‑140 (MQ=255)                                                                            gCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAg                                                    >  2:194249/1‑140 (MQ=255)                                                                               gggCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTa                                                 <  2:224128/140‑1 (MQ=255)                                                                                  ccTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCg                                              >  1:1147662/1‑140 (MQ=255)                                                                                          acacCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAAttt                                      >  1:1055531/1‑140 (MQ=255)                                                                                                tgtTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGcc                                >  2:284026/1‑140 (MQ=255)                                                                                                tgtTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGcc                                >  1:642347/1‑140 (MQ=255)                                                                                                tgtTCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGcc                                >  1:1168297/1‑140 (MQ=255)                                                                                                   tCGTAGATCTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGc                             <  2:199701/140‑1 (MQ=255)                                                                                                           cTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGaa                     <  1:194249/140‑1 (MQ=255)                                                                                                           cTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGaa                     <  1:1059775/140‑1 (MQ=255)                                                                                                           cTGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGGGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGaa                     <  2:281401/140‑1 (MQ=255)                                                                                                            tGACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAAc                    >  2:815750/1‑140 (MQ=255)                                                                                                             gNCGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACg                   >  1:651054/1‑140 (MQ=255)                                                                                                             gACGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACg                   <  2:1201967/140‑1 (MQ=255)                                                                                                              aCGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACgg                  <  2:94701/140‑1 (MQ=255)                                                                                                              aCGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACgg                  >  2:79087/1‑140 (MQ=255)                                                                                                              aCGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACgg                  >  2:689347/1‑140 (MQ=255)                                                                                                              aCGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACgg                  <  2:582955/140‑1 (MQ=255)                                                                                                               cGGTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACGGt                 >  1:874202/1‑140 (MQ=255)                                                                                                                ggTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACGGTc                <  2:1055531/140‑1 (MQ=255)                                                                                                                ggTGAATTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACGGTc                >  1:88701/1‑140 (MQ=255)                                                                                                                    aaTTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGaa                     >  2:1094990/1‑131 (MQ=255)                                                                                                                    aaTTTTACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGaa                     <  1:1094990/131‑1 (MQ=255)                                                                                                                      ttttACCCTCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACGGTCATGATc          <  2:874202/140‑1 (MQ=255)                                                                                                                              tCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACGGTCATGATCACTTccgc  >  1:256333/1‑140 (MQ=255)                                                                                                                              tCGAGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACGGTCATGATCACTTccgc  >  1:1090806/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                         CATTGGTGAAGGTTTCGAGGCTGGGCCAGAAACGCACCATGGTGCCGGTTTTTTCAGTCTCGCCGGTAACCGCCAGCGGGGCCTGCGGTACACCGTGTTCGTAGATCTGACGGTGAATTTTACCCTCGCGCTGGATAACCAGCTCCAGTTTTTGCGACAGGGCGTTTACTACCGAAACACCAACGCCGTGCAGACCGCCGGACACTTTATAGGAGTTATCGTCAAATTTACCGCCTGCGTGCAGAACGGTCATGATCACTTCCGC  >  NC_000913/3879585‑3879849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑