Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,216,897	C→A	G171C (GGC→TGC)	yehX ←	glycine betaine ABC transporter ATP binding subunit YehX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,216,897	0	C	A	100.0%	109.1 / NA	33	G171C (GGC→TGC)	yehX	glycine betaine ABC transporter ATP binding subunit YehX
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (18/15);  total (18/15)

CTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATT  >  NC_000913/2216763‑2217032                                                                                                                                       |                                                                                                                                        ctgcACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCagcgcg                                                                                                                                    <  1:508620/140‑1 (MQ=255) ctgcACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCagcgcg                                                                                                                                    <  1:800290/140‑1 (MQ=255) ctgcACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCagcgcg                                                                                                                                    <  1:863513/140‑1 (MQ=255)            cACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTcc                                                                                                                         >  2:947711/1‑140 (MQ=255)                   aTCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTgcgc                                                                                                                  >  1:391064/1‑140 (MQ=255)                   aTCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTgcgc                                                                                                                  >  2:17023/1‑140 (MQ=255)                   aTCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTgcgc                                                                                                                  >  1:805776/1‑140 (MQ=255)                   aTCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGAACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTgcgc                                                                                                                  >  1:853188/1‑140 (MQ=255)                         cAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAg                                                                                                            >  1:853199/1‑140 (MQ=255)                              ccAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCa                                                                                                       <  2:805776/140‑1 (MQ=255)                                  aTGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCa                                                                                                   <  2:380915/140‑1 (MQ=255)                                      tCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAg                                                                                               >  2:365713/1‑140 (MQ=255)                                      tCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAg                                                                                               <  1:242333/140‑1 (MQ=255)                                      tCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAg                                                                                               <  1:752634/140‑1 (MQ=255)                                       cTGCCAGCCGTAGCGCCTCATCAATATCATTAGAGACCAGCACAATGGGACTCCCCAGCAAACGGCGAATGCGCGTCTTCTCTTGCTGCAACGCGCATCGCGTTACTGCGTGCAGTGCGCCCAAAGGTTCATCCATTAgg                                                                                              >  2:345117/1‑139 (MQ=255)                                       cTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGt                                                                                              >  2:796587/1‑140 (MQ=255)                                            aGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGAc                                                                                         <  1:486922/140‑1 (MQ=255)                                                    cgcCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGAt                                                                                 >  1:141256/1‑140 (MQ=255)                                                                    tGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCAcg                                                                 >  2:869042/1‑140 (MQ=255)                                                                       gTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACgcgc                                                              <  2:853199/140‑1 (MQ=255)                                                                       gTGACCAGCACAATGGTACGCCCCAGCAAACGGGGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCAGCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACgcgc                                                              <  1:947711/140‑1 (MQ=255)                                                                          aCCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCAc                                                           >  2:248698/1‑140 (MQ=255)                                                                                    tGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGt                                                 <  1:17023/140‑1 (MQ=255)                                                                                    tGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGt                                                 <  2:28781/140‑1 (MQ=255)                                                                                         cGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtgctg                                            >  2:724941/1‑140 (MQ=255)                                                                                         cGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtgctg                                            >  1:921093/1‑140 (MQ=255)                                                                                         cGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTtgctg                                            >  1:729029/1‑140 (MQ=255)                                                                                           ccccAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGGGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGAACGCGCCACTCCCACACGTTgctgct                                          <  2:204127/140‑1 (MQ=255)                                                                                            cccAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGctgctg                                         >  2:448407/1‑140 (MQ=255)                                                                                               aGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGacc                                      >  2:664660/1‑140 (MQ=255)                                                                                                gCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGacca                                     >  2:546175/1‑140 (MQ=255)                                                                                                      ggTGAATGCGCGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGaa                               <  1:664660/140‑1 (MQ=255)                                                                                                             gcgcGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGAt                        <  2:2648/140‑1 (MQ=255)                                                                                                             gcgcGTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGAt                        <  2:729029/140‑1 (MQ=255)                                                                                                              cgcgTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATg                       <  2:547566/140‑1 (MQ=255)                                                                                                              cgcgTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATg                       <  2:853188/140‑1 (MQ=255)                                                                                                              cgcgTCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATg                       <  1:224735/140‑1 (MQ=255)                                                                                                                  tCATCTCTTGTTGCAACGCGCAGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGa                   >  2:712787/1‑140 (MQ=255)                                                                                                                                   gcgcagcgcGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAAtt  >  2:192432/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        CTGCACTACTTCACCGTGATCCATCAATACCAGATGTTCTGCCAGCCGTAGCGCCTCATCAATATCATGAGTGACCAGCACAATGGTACGCCCCAGCAAACGGTGAATGCGCGTCATCTCTTGTTGCAACGCGCCGCGCGTTACCGGGTCCAGTGCGCCAAAAGGTTCATCCATTAGTAAGACTTGCGGATCGGCAGCCAGTGCACGCGCCACTCCCACACGTTGCTGCTGACCACCGGAAAGCTGATGCGGATAACGCTCACGCAAATT  >  NC_000913/2216763‑2217032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑