| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 411,866 | C→A | A206S (GCC→TCC) | araJ ← | putative transport protein AraJ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 411,866 | 0 | C | A | 97.9% | 162.1 / ‑2.7 | 48 | A206S (GCC→TCC) | araJ | putative transport protein AraJ |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (25/22); total (26/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.80e-01 | |||||||||||
CATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATA > NC_000913/411738‑412000 | cATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAAcc > 1:7706/1‑140 (MQ=255) aTCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCa < 2:895898/140‑1 (MQ=255) tCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCAc > 1:582363/1‑140 (MQ=255) aCTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCt > 2:86650/1‑140 (MQ=255) aCTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCt > 1:811385/1‑140 (MQ=255) aataaAGGTCATCGCCGTTTCCGAAAAACCGG‑AATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAACTGAACCGCGGGG‑CGGGCTTCGCaaaaaaaga > 2:181018/1‑136 (MQ=255) aGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAAt > 1:645693/1‑140 (MQ=255) aTCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTc > 2:316851/1‑140 (MQ=255) aTCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTc > 2:562224/1‑140 (MQ=255) aTCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTc > 2:814778/1‑140 (MQ=255) tCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTcg < 2:811385/140‑1 (MQ=255) cGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTcgc > 2:772448/1‑140 (MQ=255) cGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTcgc > 1:755234/1‑140 (MQ=255) cGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTcgc > 1:605104/1‑140 (MQ=255) ccGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCgcgc < 1:562224/140‑1 (MQ=255) ccGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCgcgc < 2:645693/140‑1 (MQ=255) cGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCa < 1:35584/140‑1 (MQ=255) ttCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGAt < 1:316851/140‑1 (MQ=255) ccGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGc < 2:605104/140‑1 (MQ=255) aaaTAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCtcg < 1:772448/140‑1 (MQ=255) gCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCAcc < 2:546613/140‑1 (MQ=255) gCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCAcc < 2:206376/140‑1 (MQ=255) cTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCAccc < 2:755234/140‑1 (MQ=255) tACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCaaa > 2:192631/1‑140 (MQ=255) tACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCaaa > 1:95845/1‑140 (MQ=255) tACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCaaa > 1:268859/1‑140 (MQ=255) tACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCaaa > 1:678052/1‑140 (MQ=255) aCGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCaaaa > 1:548758/1‑140 (MQ=255) cGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCaaaaa > 1:480870/1‑140 (MQ=255) gCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGa < 2:32910/140‑1 (MQ=255) cTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGAc > 1:773898/1‑140 (MQ=255) cTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGAc > 1:469509/1‑140 (MQ=255) cTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGAc < 2:268859/140‑1 (MQ=255) aaCCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAAATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGa > 2:337397/1‑140 (MQ=255) ggCAAACACACTTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCa > 1:236570/1‑140 (MQ=255) ggCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGa > 2:105139/1‑131 (MQ=255) ggCAAACACACCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGa < 1:105139/131‑1 (MQ=255) cacacCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCg < 2:548758/140‑1 (MQ=255) cacCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCa < 1:667789/140‑1 (MQ=255) cacCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCa < 2:480870/140‑1 (MQ=255) cacCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCa < 1:447441/140‑1 (MQ=255) cacCTGCGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCa < 1:370099/140‑1 (MQ=255) cGTTGCCAAACATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTa > 2:222740/1‑140 (MQ=255) aCATCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCg < 2:487125/140‑1 (MQ=255) aTCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGAt < 1:365514/140‑1 (MQ=255) aTCGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCCCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGAt < 1:814778/140‑1 (MQ=255) cGTGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCa > 2:483564/1‑140 (MQ=255) tGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAAt > 1:515163/1‑140 (MQ=255) tGGCGGAGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAAt > 1:687834/1‑140 (MQ=255) ggcggaGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATa < 2:687834/140‑1 (MQ=255) | CATCCCTAGCCCAACTAACATCATAATAAAGGTCATCGCCGTTTCCGAAAAACCGGAAATAAACATCATGTATGGCTTTACGTAGCTGAACCAGGCAAACACACCTGCGTTGCCAAACATCGTGGCGGCGAAAATTAACCACGGGGCCGGGCTGCGCAAAAAGTGAAATTGTTCGCGCAGATTTCCTTTCGCCTCGTCGCGAATATCTGGCACCCAAAAATAGACCGATGCCATCACCGCAATATTAAAAACAGCGATCAATA > NC_000913/411738‑412000 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |