Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,539,178 G→T R108R (CGC→CGA)  nanC ← N‑acetylneuraminic acid outer membrane channel

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,539,1780GT100.0% 263.2 / NA 75R108R (CGC→CGA) nanCN‑acetylneuraminic acid outer membrane channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (38/37);  total (38/37)

CCCAACGATGAACGTTATCACGAGACATATCACCGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTAGCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAATGGTA  >  NC_000913/4539042‑4539313
                                                                                                                                        |                                                                                                                                       
cccAACGATGAACGTTATCACGAGACATATCACCGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCgt                                                                                                                                      >  1:83584/1‑140 (MQ=255)
  cAACGATGAACGTTATCACGAGACATATCACCGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCATATCgtat                                                                                                                                    >  1:431194/1‑138 (MQ=255)
          aaCGTTATCACGAGACATATCACCGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTtgc                                                                                                                            >  1:783421/1‑140 (MQ=255)
                     gagaCATATCACCGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGc                                                                                                                 <  1:665988/140‑1 (MQ=255)
                            atCACCGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACa                                                                                                          >  1:909929/1‑140 (MQ=255)
                            atCACCGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACa                                                                                                          >  2:393925/1‑140 (MQ=255)
                                 cGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTccc                                                                                                     >  2:863003/1‑140 (MQ=255)
                                   gATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCgg                                                                                                   >  2:728458/1‑140 (MQ=255)
                                   gATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCgg                                                                                                   >  1:717201/1‑140 (MQ=255)
                                   gATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCgg                                                                                                   >  2:247654/1‑140 (MQ=255)
                                       aGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGgcgc                                                                                               <  1:735981/140‑1 (MQ=255)
                                        gTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCa                                                                                              <  2:228844/140‑1 (MQ=255)
                                        gTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCa                                                                                              <  1:181021/140‑1 (MQ=255)
                                        gTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCa                                                                                              <  1:947782/140‑1 (MQ=255)
                                        gTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTGTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCa                                                                                              <  2:220487/140‑1 (MQ=255)
                                          cTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCAcc                                                                                            <  2:558947/140‑1 (MQ=255)
                                              ttgtCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTcc                                                                                        >  1:892298/1‑140 (MQ=255)
                                                   ggTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCg                                                                                           >  1:656999/1‑132 (MQ=255)
                                                   ggTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCg                                                                                           <  2:656999/132‑1 (MQ=255)
                                                   ggTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGa                                                                                   >  2:276714/1‑140 (MQ=255)
                                                   ggTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGa                                                                                   >  2:471154/1‑140 (MQ=255)
                                                   ggTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGa                                                                                   >  1:919365/1‑140 (MQ=255)
                                                   ggTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGa                                                                                   >  2:619167/1‑140 (MQ=255)
                                                   ggTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGACCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGa                                                                                   >  2:746915/1‑140 (MQ=255)
                                                   ggTAAGCTTTCCAGTCGTAACAATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGa                                                                                   >  1:571079/1‑140 (MQ=255)
                                                      aaGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGatca                                                                                <  2:567831/140‑1 (MQ=255)
                                                           ttCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAg                                                                           >  1:193402/1‑140 (MQ=255)
                                                                   gTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCATATCGTATGCCGTTTCTTCCTTGATACGAAAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATcg                                                                   >  1:947355/1‑140 (MQ=255)
                                                                     aaCGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATcgcg                                                                 <  2:909929/140‑1 (MQ=255)
                                                                     aaCGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATcgcg                                                                 <  1:728458/140‑1 (MQ=255)
                                                                      aCGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGt                                                                >  1:476612/1‑140 (MQ=255)
                                                                         aTAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAAt                                                             <  1:247654/140‑1 (MQ=255)
                                                                           aGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTa                                                           >  2:455638/1‑140 (MQ=255)
                                                                              gAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACt                                                        <  1:863003/140‑1 (MQ=255)
                                                                                aTGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCTGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTc                                                      <  2:889547/140‑1 (MQ=255)
                                                                                aTGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTc                                                      <  2:111973/140‑1 (MQ=255)
                                                                                   ccAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAAc                                                   <  2:571079/140‑1 (MQ=255)
                                                                                    cAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAAcc                                                  >  2:264708/1‑140 (MQ=255)
                                                                                     agaaTTAAGATCTTTTGTCGCGTGCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCt                                                 <  2:947355/138‑1 (MQ=255)
                                                                                     aaaaTTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCt                                                 <  1:77099/140‑1 (MQ=255)
                                                                                     aaaaTTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCt                                                 <  2:121751/140‑1 (MQ=255)
                                                                                        aTTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGaa                                              <  1:227059/140‑1 (MQ=255)
                                                                                         ttAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGTCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCGTTGGTCATCAAGTTTACTCGGGTAATTATCTTCATCCTGAAc                                             <  2:431194/140‑1 (MQ=255)
                                                                                            aGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAAcatc                                          >  1:47128/1‑140 (MQ=255)
                                                                                             gATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAAcatca                                         >  1:430154/1‑140 (MQ=255)
                                                                                               tCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCAtt                                       <  1:393925/140‑1 (MQ=255)
                                                                                                  tttGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAg                                    >  2:598591/1‑140 (MQ=255)
                                                                                                   ttGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGt                                   >  2:936338/1‑140 (MQ=255)
                                                                                                    tGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGGGTAATTAACTTCAACCTGAACATCATTGAGTg                                  <  2:932210/140‑1 (MQ=255)
                                                                                                    tGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTg                                  <  2:919365/140‑1 (MQ=255)
                                                                                                         cgTCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCa                             >  1:705107/1‑140 (MQ=255)
                                                                                                           tCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCTGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCAtt                           >  2:334134/1‑140 (MQ=255)
                                                                                                           tCCCAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCAtt                           >  1:446503/1‑140 (MQ=255)
                                                                                                            cccAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCAttt                          <  1:302510/140‑1 (MQ=255)
                                                                                                            cccAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCAttt                          <  1:305780/140‑1 (MQ=255)
                                                                                                             ccAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCAtttt                         >  1:458506/1‑140 (MQ=255)
                                                                                                              cAGGACAGTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTc                        <  1:471154/140‑1 (MQ=255)
                                                                                                                     gTTTTACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTtta                 <  2:182435/140‑1 (MQ=255)
                                                                                                                      ttttACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTttat                >  2:135894/1‑140 (MQ=255)
                                                                                                                      ttttACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTttat                >  1:773634/1‑140 (MQ=255)
                                                                                                                      ttttACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTttat                >  2:484173/1‑140 (MQ=255)
                                                                                                                      ttttACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTttat                >  1:786835/1‑140 (MQ=255)
                                                                                                                       tttACGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCTTTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTttatt               <  2:556581/140‑1 (MQ=255)
                                                                                                                          aCGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATc            <  2:585657/140‑1 (MQ=255)
                                                                                                                           cGTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCa           >  1:265483/1‑140 (MQ=255)
                                                                                                                            gTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCAt          <  1:874321/140‑1 (MQ=255)
                                                                                                                            gTAGGGTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCAt          <  2:786835/140‑1 (MQ=255)
                                                                                                                               gggTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGaa       <  2:717201/140‑1 (MQ=255)
                                                                                                                                ggTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAAt      >  1:344265/1‑140 (MQ=255)
                                                                                                                                ggTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAAt      >  1:629781/1‑140 (MQ=255)
                                                                                                                                ggTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAAt      >  2:41285/1‑140 (MQ=255)
                                                                                                                                 gTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAATg     <  2:705107/140‑1 (MQ=255)
                                                                                                                                 gTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAATg     <  1:648086/140‑1 (MQ=255)
                                                                                                                                 gTCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAATg     <  1:598591/140‑1 (MQ=255)
                                                                                                                                  tCCGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAATgg    <  1:484173/140‑1 (MQ=255)
                                                                                                                                   ccGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAATGGt   <  1:97588/140‑1 (MQ=255)
                                                                                                                                    cGTATCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAATGGTa  <  1:936338/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CCCAACGATGAACGTTATCACGAGACATATCACCGGATAAGTCTTGTTGTCGGTAAGCTTTCCAGTCGTAACGATAGCGAATGCCAAAATTAAGATCTTTTGTCGCGTCCCAGGACAGTTTTACGTAGGGTCCGTAGCGTGTGCCGTTGCTGCTAAAATGCGTTAACATTCCCGGGCGCACCGTCCATTGATCATCAAGTTTAATCGCGTAATTAACTTCAACCTGAACATCATTGAGTGCGGCATTTTCCTTTTTATTATCATGAATGGTA  >  NC_000913/4539042‑4539313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑