Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	534,937	G→T	R341L (CGT→CTT)	gcl →	glyoxylate carboligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	534,937	0	G	T	100.0%	247.1 / NA	69	R341L (CGT→CTT)	gcl	glyoxylate carboligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/33);  total (36/33)

CGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCG  >  NC_000913/534801‑535071                                                                                                                                         |                                                                                                                                       cgcAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGtcttct                                                                                                                                     >  2:468019/1‑140 (MQ=255)  gcAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTg                                                                                                                                    <  2:709225/140‑1 (MQ=255)    aaaaTCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGcc                                                                                                                                  <  2:409851/140‑1 (MQ=255)                      attgaGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGt                                                                                                                >  1:831457/1‑140 (MQ=255)                         gaGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGc                                                                                                             <  2:32583/140‑1 (MQ=255)                          aGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGcc                                                                                                            >  2:409412/1‑140 (MQ=255)                            ccGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGTGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGa                                                                                                          >  1:156496/1‑140 (MQ=255)                            ccGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGc                                                                                                             >  2:312749/1‑137 (MQ=255)                            ccGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGc                                                                                                             <  1:312749/137‑1 (MQ=255)                            ccGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGa                                                                                                          >  2:403191/1‑140 (MQ=255)                            ccGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGa                                                                                                          >  1:630534/1‑140 (MQ=255)                            ccGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGa                                                                                                          >  1:362643/1‑140 (MQ=255)                              gACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACt                                                                                                        <  2:200375/140‑1 (MQ=255)                              gACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACt                                                                                                        >  1:46279/1‑140 (MQ=255)                                cGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGc                                                                                                      >  1:244879/1‑140 (MQ=255)                                    aaTTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCcagc                                                                                                  <  1:468019/140‑1 (MQ=255)                                      ttGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCcagcag                                                                                                >  1:81275/1‑140 (MQ=255)                                          tCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTa                                                                                            >  2:124559/1‑140 (MQ=255)                                             cgTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAAc                                                                                         >  1:482309/1‑140 (MQ=255)                                                  tgtgTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACt                                                                                    <  1:403191/140‑1 (MQ=255)                                                   gtgtCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACtt                                                                                   >  2:758896/1‑140 (MQ=255)                                                    tgtCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACttt                                                                                  >  2:704462/1‑140 (MQ=255)                                                      tCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTtgc                                                                                >  2:294340/1‑140 (MQ=255)                                                           aTCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTgcgc                                                                           >  1:165608/1‑140 (MQ=255)                                                              tcGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCaaa                                                                        >  1:897884/1‑140 (MQ=255)                                                               cGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCaaaa                                                                       <  2:630534/140‑1 (MQ=255)                                                                ggTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAAc                                                                      >  2:379730/1‑140 (MQ=255)                                                                 gTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAAcc                                                                     <  2:244879/140‑1 (MQ=255)                                                                  tATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAAccc                                                                    <  1:394430/140‑1 (MQ=255)                                                                   aTTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCa                                                                   <  1:409412/140‑1 (MQ=255)                                                                     tGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACt                                                                 >  2:173814/1‑140 (MQ=255)                                                                       tctcTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTc                                                               >  2:53243/1‑140 (MQ=255)                                                                       tctcTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTc                                                               >  1:236882/1‑140 (MQ=255)                                                                       tctcTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTc                                                               >  2:728377/1‑140 (MQ=255)                                                                           tGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACa                                                           <  1:704462/140‑1 (MQ=255)                                                                            gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCAc                                                                  <  1:788944/132‑1 (MQ=255)                                                                            gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCAc                                                                  >  2:788944/1‑132 (MQ=255)                                                                            gATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACaa                                                          >  1:722854/1‑140 (MQ=255)                                                                               gCTAAAGCGGTGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGATTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGt                                                       <  2:156496/140‑1 (MQ=255)                                                                                cTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTg                                                      >  1:170802/1‑140 (MQ=255)                                                                                   aaGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCg                                                   >  2:903141/1‑140 (MQ=255)                                                                                        gcgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGaa                                              >  2:686840/1‑140 (MQ=255)                                                                                         cgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGaaa                                             >  1:264639/1‑140 (MQ=255)                                                                                         cgcTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGaaa                                             >  2:690636/1‑140 (MQ=255)                                                                                                 ctgctgGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAgcg                                     <  2:236882/140‑1 (MQ=255)                                                                                                 ctgctgGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAgcg                                     <  1:728377/140‑1 (MQ=255)                                                                                                  tgctgGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAgcgc                                    <  1:173814/140‑1 (MQ=255)                                                                                                   gctgGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGcgcg                                   <  1:124559/140‑1 (MQ=255)                                                                                                      ggTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCgtgt                                <  2:722854/140‑1 (MQ=255)                                                                                                      ggTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACATGCCGGTGAAACCGCAGCGCgtgt                                <  2:897884/140‑1 (MQ=255)                                                                                                       gTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTa                               >  1:778213/1‑140 (MQ=255)                                                                                                        ttGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTag                              >  2:256834/1‑139 (MQ=255)                                                                                                        ttGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTAt                              >  1:121054/1‑140 (MQ=255)                                                                                                         tGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATg                             <  1:758896/140‑1 (MQ=255)                                                                                                           aaGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAGCGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGaa                           <  2:165608/140‑1 (MQ=255)                                                                                                           aaGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGaa                           <  2:362643/140‑1 (MQ=255)                                                                                                           aaGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGaa                           <  2:81275/140‑1 (MQ=255)                                                                                                            aGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAag                          >  1:614950/1‑140 (MQ=255)                                                                                                            aGTGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAag                          >  1:147989/1‑140 (MQ=255)                                                                                                              tGGCGCAGGAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAagag                        <  2:170802/140‑1 (MQ=255)                                                                                                                     ggAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACa                 <  1:686840/140‑1 (MQ=255)                                                                                                                     ggAGATGCAAAAAGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACa                 <  1:125395/140‑1 (MQ=255)                                                                                                                              aaaaGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTg        >  2:577766/1‑140 (MQ=255)                                                                                                                                aaGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGt      <  1:294340/140‑1 (MQ=255)                                                                                                                                aaGCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGt      <  1:690636/140‑1 (MQ=255)                                                                                                                                  gCGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcg    <  2:121054/140‑1 (MQ=255)                                                                                                                                   cGGGTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcgc   <  2:147989/140‑1 (MQ=255)                                                                                                                                    gggTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcgcg  >  2:875490/1‑140 (MQ=255)                                                                                                                                    gggTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcgcg  <  2:778213/140‑1 (MQ=255)                                                                                                                                    gggTCTTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTcgcg  <  1:53243/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       CGCAAAATCGTTCATATTGATATTGAGCCGACGCAAATTGGTCGCGTGCTGTGTCCGGATCTCGGTATTGTCTCTGATGCTAAAGCGGCGCTGACACTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCGGGTCGTCTGCCGTGTCGTAAAGAATGGGTCGCCGACTGCCAGCAGCGTAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTGAAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGTCGCG  >  NC_000913/534801‑535071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑