Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	992,686	G→T	A689S (GCT→TCT)	pepN →	aminopeptidase N

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	992,686	0	G	T	100.0%	143.8 / NA	41	A689S (GCT→TCT)	pepN	aminopeptidase N
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (23/18);  total (23/18)

ACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCGCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCA  >  NC_000913/992554‑992819                                                                                                                                     |                                                                                                                                      actcGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTgg                                                                                                                                <  1:125616/140‑1 (MQ=255)  ctcGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGt                                                                                                                               <  1:527138/140‑1 (MQ=255)    cGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAAATATTGCAAAACGGACTCTGCGTAATTGCTGCCTGCGTTTCCTCTCTTTTGGTGa                                                                                                                             >  2:798290/1‑140 (MQ=255)      tACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGaaa                                                                                                                           >  2:506905/1‑140 (MQ=255)      tACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGaaa                                                                                                                           <  2:486621/140‑1 (MQ=255)           tGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCAt                                                                                                                      >  1:320158/1‑140 (MQ=255)             gCGACTGAACTGGTGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCt                                                                                                                    >  2:901175/1‑140 (MQ=255)             gCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCt                                                                                                                    >  2:537329/1‑140 (MQ=255)             gCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCt                                                                                                                    >  1:885870/1‑140 (MQ=255)               gACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGGACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCGGCCTGGGTTTCCTCTCTTTTGGTGAAACGCATCTgg                                                                                                                  >  2:723876/1‑140 (MQ=255)                  tGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTg                                                                                                               <  1:798290/140‑1 (MQ=255)                              tGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGa                                                                                                   >  1:157652/1‑140 (MQ=255)                              tGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGa                                                                                                   >  2:131142/1‑140 (MQ=255)                              tGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGa                                                                                                   <  1:467917/140‑1 (MQ=255)                                   tACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGagcaag                                                                                              <  1:723876/140‑1 (MQ=255)                                   tACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGagcaag                                                                                              <  1:455106/140‑1 (MQ=255)                                               aCAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGaa                                                                                  <  2:322680/140‑1 (MQ=255)                                                  aCGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCa                                                                               >  1:265495/1‑140 (MQ=255)                                                  aCGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCa                                                                               <  1:506905/140‑1 (MQ=255)                                                  aCGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCa                                                                               <  1:422357/140‑1 (MQ=255)                                                    gcgAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCaaa                                                                             <  2:480253/140‑1 (MQ=255)                                                                  cGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATg                                                               >  2:510292/1‑140 (MQ=255)                                                                  cGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATg                                                               >  2:705839/1‑140 (MQ=255)                                                                    aGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATgcg                                                             >  2:26127/1‑140 (MQ=255)                                                                           tgtTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTggcgg                                                      >  1:724385/1‑140 (MQ=255)                                                                           tgtTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTggcgg                                                      >  2:374736/1‑140 (MQ=255)                                                                                  cATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTc                                               >  2:503710/1‑140 (MQ=255)                                                                                     gAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTgc                                            >  2:554707/1‑140 (MQ=255)                                                                                     gAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTgc                                            >  1:836874/1‑140 (MQ=255)                                                                                           atTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGt                                      <  1:705839/140‑1 (MQ=255)                                                                                           atTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGt                                      <  1:554707/140‑1 (MQ=255)                                                                                             tGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTg                                    <  1:374736/140‑1 (MQ=255)                                                                                                  aaCGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGcc                               >  2:695638/1‑139 (MQ=255)                                                                                                   aCGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGcac                              >  2:64224/1‑140 (MQ=255)                                                                                                             cGTAATGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTg                    <  2:320158/140‑1 (MQ=255)                                                                                                                aaTGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCCCAGCCGCCCTGccc                 >  2:276970/1‑139 (MQ=255)                                                                                                                 aTGCCTGCCTGCGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGt                >  1:364599/1‑140 (MQ=255)                                                                                                                       gcctgcGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGAcgcg          <  1:510292/140‑1 (MQ=255)                                                                                                                       gcctgcGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGAcgcg          >  1:652274/1‑140 (MQ=255)                                                                                                                       gcctgcGTTTCCTCTCTTTTAGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCCCAGCCGCCCTGCCGTGAcgcg          >  2:498162/1‑140 (MQ=255)                                                                                                                        cctgcGTTTCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACgcgc         <  2:928732/140‑1 (MQ=255)                                                                                                                              tttCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGc   <  2:724385/140‑1 (MQ=255)                                                                                                                               ttCCTCTCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCa  >  1:927555/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      ACTCGTACTCTGGCGACTGAACTGGCGGATGAGCTACTGGCTATTTACAACGCGAATTACCAGAGCGAGTACCGTGTTGAGCATGAAGATATTGCAAAACGCACTCTGCGTAATGCCTGCCTGCGTTTCCTCGCTTTTGGTGAAACGCATCTGGCTGATGTGCTGGTGAGCAAGCAGTTCCACGAAGCAAACAATATGACTGATGCGCTGGCGGCGCTTTCTGCGGCGGTTGCCGCACAGCTGCCTTGCCGTGACGCGCTGATGCA  >  NC_000913/992554‑992819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑