BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,016,447	C→A	L194I (CTC→ATC)	fliH →	flagellar biosynthesis protein FliH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,016,447	0	C	A	100.0%	356.4 / NA	102	L194I (CTC→ATC)	fliH	flagellar biosynthesis protein FliH
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (56/46); total (56/46)

Only 100 of 106 total aligned reads displayed.

TGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAAC  >  NC_000913/2016316‑2016582

tgttgCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCt                                                                                                                                 >  1:459505/1‑140 (MQ=255)

 gttgCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTg                                                                                                                                >  1:186126/1‑140 (MQ=255)

  ttgCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTgg                                                                                                                               <  1:146272/140‑1 (MQ=255)

  ttgCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTgg                                                                                                                               <  2:790382/140‑1 (MQ=255)

    gcagcaAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTggcg                                                                                                                             >  1:625669/1‑140 (MQ=255)

      agcaAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTggcggc                                                                                                                           >  2:824548/1‑140 (MQ=255)

       gcaAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCt                                                                                                                          >  2:1235142/1‑140 (MQ=255)

         aaGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGt                                                                                                                        >  2:27633/1‑140 (MQ=255)

              ccGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGt                                                                                                                   <  2:813836/140‑1 (MQ=255)

                  tATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTcc                                                                                                               >  1:1118850/1‑140 (MQ=255)

                      cAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTggcggc                                                                                                                           <  2:744531/124‑1 (MQ=255)

                      cAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTggcggc                                                                                                                           >  1:744531/1‑124 (MQ=255)

                         cGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATg                                                                                                        >  2:1305131/1‑140 (MQ=255)

                         cGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATg                                                                                                        >  1:1237869/1‑140 (MQ=255)

                         cGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATg                                                                                                        >  1:831916/1‑140 (MQ=255)

                         cGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATg                                                                                                        >  1:1411104/1‑140 (MQ=255)

                              aaCCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGc                                                                                                   >  1:1052130/1‑140 (MQ=255)

                               aCCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCg                                                                                                  <  1:1260760/140‑1 (MQ=255)

                               aCCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCg                                                                                                  <  2:1121671/140‑1 (MQ=255)

                               aCCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCg                                                                                                  <  2:557235/140‑1 (MQ=255)

                               aCCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCg                                                                                                  <  1:824548/140‑1 (MQ=255)

                               aCCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCg                                                                                                  <  2:186126/140‑1 (MQ=255)

                                   caGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGAtct                                                                                              >  2:614788/1‑140 (MQ=255)

                                   caGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGAtct                                                                                              >  2:1013662/1‑140 (MQ=255)

                                      cTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGa                                                                                           >  2:693466/1‑140 (MQ=255)

                                      cTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGa                                                                                           >  1:771190/1‑140 (MQ=255)

                                      cTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGa                                                                                           >  2:880340/1‑140 (MQ=255)

                                           cgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCa                                                                                      >  1:1210719/1‑140 (MQ=255)

                                           cgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCa                                                                                      <  2:1311392/140‑1 (MQ=255)

                                           cgTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCa                                                                                      >  2:873899/1‑140 (MQ=255)

                                             tGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAgt                                                                                    <  2:1023580/140‑1 (MQ=255)

                                                      atgatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACt                                                                           <  1:192949/140‑1 (MQ=255)

                                                        gatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCg                                                                         <  1:693466/140‑1 (MQ=255)

                                                        gatCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCGGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCg                                                                         <  2:625669/140‑1 (MQ=255)

                                                          tCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCt                                                                       <  1:1305131/140‑1 (MQ=255)

                                                          tCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCt                                                                       <  1:1235142/140‑1 (MQ=255)

                                                            tGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTgg                                                                     >  1:699798/1‑140 (MQ=255)

                                                            tGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTACATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCg                                                                         <  2:931400/136‑1 (MQ=255)

                                                            tGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTACATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCg                                                                         >  1:931400/1‑136 (MQ=255)

                                                             gCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGc                                                                    >  2:73232/1‑140 (MQ=255)

                                                                aCGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAg                                                                 >  2:475361/1‑140 (MQ=255)

                                                                   tgtgGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACt                                                                           <  2:985826/127‑1 (MQ=255)

                                                                   tgtgGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACt                                                                           >  1:985826/1‑127 (MQ=255)

                                                                   tgtgGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAAc                                                              >  1:49997/1‑140 (MQ=255)

                                                                   tgtgGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAAc                                                              >  1:1156625/1‑140 (MQ=255)

                                                                   tgtgGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAAc                                                              >  1:1179954/1‑140 (MQ=255)

                                                                   tgtgGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCACTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAAc                                                              >  1:546007/1‑140 (MQ=255)

                                                                     tgGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAActc                                                            >  1:1033486/1‑140 (MQ=255)

                                                                     tgGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAActc                                                            >  2:134400/1‑140 (MQ=255)

                                                                            tatGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTc                                                     >  2:1218551/1‑140 (MQ=255)

                                                                            tatGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTc                                                     >  1:1229512/1‑140 (MQ=255)

                                                                            tatGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTc                                                     >  2:829860/1‑140 (MQ=255)

                                                                             atGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCt                                                    >  1:1327131/1‑140 (MQ=255)

                                                                             atGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCt                                                    >  1:1055023/1‑140 (MQ=255)

                                                                                cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 <  2:1118850/140‑1 (MQ=255)

                                                                                cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 <  2:802950/140‑1 (MQ=255)

                                                                                cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 <  2:1179954/140‑1 (MQ=255)

                                                                                cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 >  1:1364736/1‑140 (MQ=255)

                                                                                cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 <  2:47892/140‑1 (MQ=255)

                                                                                cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 <  2:213540/140‑1 (MQ=255)

                                                                                cTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTGGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 <  2:1362739/140‑1 (MQ=255)

                                                                                 tCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgca                                                >  1:761886/1‑140 (MQ=255)

                                                                                  cGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgcag                                               >  1:826356/1‑140 (MQ=255)

                                                                                   ggCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgcagc                                              <  2:771190/140‑1 (MQ=255)

                                                                                    gcgcTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgcagca                                             >  2:304652/1‑140 (MQ=255)

                                                                                     cgcTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 <  1:404387/135‑1 (MQ=255)

                                                                                     cgcTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 >  2:404387/1‑135 (MQ=255)

                                                                                      gcTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCAcc                                           <  1:873899/140‑1 (MQ=255)

                                                                                        tACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAg                                         <  2:826356/140‑1 (MQ=255)

                                                                                          ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 >  2:254945/1‑130 (MQ=255)

                                                                                          ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGgc                                                 <  1:254945/130‑1 (MQ=255)

                                                                                          ccTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGa                                       <  2:1229512/140‑1 (MQ=255)

                                                                                           cTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAg                                      >  1:541016/1‑140 (MQ=255)

                                                                                             tAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAgtg                                    <  2:1411104/140‑1 (MQ=255)

                                                                                                    gCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                             >  1:606316/1‑140 (MQ=255)

                                                                                                    gCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                             >  1:897204/1‑140 (MQ=255)

                                                                                                    gCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                             >  2:1147951/1‑140 (MQ=255)

                                                                                                    gCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                             >  2:1233656/1‑140 (MQ=255)

                                                                                                    gCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                             >  2:556087/1‑140 (MQ=255)

                                                                                                    gCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                             >  2:391876/1‑140 (MQ=255)

                                                                                                    gCATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAAt                             >  1:882619/1‑140 (MQ=255)

                                                                                                     cATGGCTGGCGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATg                            >  1:767854/1‑140 (MQ=255)

                                                                                                         gctggcGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCa                        <  2:568487/140‑1 (MQ=255)

                                                                                                         gctggcGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCa                        >  1:248725/1‑140 (MQ=255)

                                                                                                         gctggcGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCa                        <  1:1233656/140‑1 (MQ=255)

                                                                                                         gctggcGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCa                        <  1:1147951/140‑1 (MQ=255)

                                                                                                          ctggcGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAACGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCAc                       >  1:1077071/1‑140 (MQ=255)

                                                                                                            ggcGCTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCAcgc                     <  2:1160688/140‑1 (MQ=255)

                                                                                                              cgcTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACgcgc                   <  1:27633/140‑1 (MQ=255)

                                                                                                               gcTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGcc                  <  2:761886/140‑1 (MQ=255)

                                                                                                                cTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCt                 >  1:119936/1‑140 (MQ=255)

                                                                                                                cTTGCGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCt                 <  1:829860/140‑1 (MQ=255)

                                                                                                                    cGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACt             <  2:546007/140‑1 (MQ=255)

                                                                                                                    cGGGGCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACt             >  2:264197/1‑140 (MQ=255)

                                                                                                                     ggggCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTc            <  2:781369/140‑1 (MQ=255)

                                                                                                                      gggCGATCCCACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCg           <  2:1384040/140‑1 (MQ=255)

                                                                                                                       ggCGATCCCACCATCCATCCTGGCGGCTGTAAAGTTTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTTGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGc          <  2:1405186/140‑1 (MQ=255)

                                                                                                                              ccaccaTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTaa   <  2:882619/140‑1 (MQ=255)

                                                                                                                               cACCATCCATCCTGGCGGTTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCGCTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAAc  <  2:1002385/140‑1 (MQ=255)

                                                                                                                               cACCATCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAAc  <  2:362096/140‑1 (MQ=255)

TGTTGCAGCAAGAACCGTTATTCAGCGGTAAACCACAGCTGCGCGTGCACCCGGATGATCTGCAACGTGTGGATGATATGCTCGGCGCTACCTTAAGTTTGCATGGCTGGCGCTTGCGGGGCGATCCCACCCTCCATCCTGGCGGCTGTAAAGTCTCCGCCGATGAAGGCGATCTCGACGCCAGTGTCGCCACTCGCTGGCAAGAACTCTGCCGTCTGGCAGCACCAGGAGTGGTGTAATGACCACGCGCCTGACTCGCTGGCTAAC  >  NC_000913/2016316‑2016582

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`