Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,417,109	C→A	A53D (GCT→GAT)	yjfP →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,417,109	0	C	A	100.0%	301.6 / NA	85	A53D (GCT→GAT)	yjfP	carboxylesterase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/49);  total (36/49)

AGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGC  >  NC_000913/4416974‑4417241                                                                                                                                        |                                                                                                                                     aGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGt                                                                                                                                  <  2:445778/140‑1 (MQ=255) aGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGt                                                                                                                                  <  2:845868/140‑1 (MQ=255) aGCTGGCAGATATTCCCGTTCTTCATGCTTATCCCGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGt                                                                                                                                  <  2:152888/140‑1 (MQ=255)  gCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGtt                                                                                                                                 <  2:19062/140‑1 (MQ=255)    tGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTg                                                                                                                               <  1:588763/140‑1 (MQ=255)      gCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCg                                                                                                                             >  1:1124620/1‑140 (MQ=255)       cAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCgg                                                                                                                            <  2:483671/140‑1 (MQ=255)        aGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCggg                                                                                                                           <  1:462207/140‑1 (MQ=255)        aGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCggg                                                                                                                           <  1:1322320/140‑1 (MQ=255)            atTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGAt                                                                                                                       <  2:908932/140‑1 (MQ=255)            atTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGAt                                                                                                                       <  2:1372078/140‑1 (MQ=255)            atTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGAt                                                                                                                       <  1:877404/140‑1 (MQ=255)              tCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCa                                                                                                                     >  2:1189765/1‑140 (MQ=255)                ccGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATg                                                                                                                   <  1:564988/140‑1 (MQ=255)                     cttcATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGa                                                                                                              <  2:1124620/140‑1 (MQ=255)                               aTCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGAt                                                                                                    >  2:759323/1‑140 (MQ=255)                               aTCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGAt                                                                                                    <  2:1195866/140‑1 (MQ=255)                               aTCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGAt                                                                                                    >  2:585956/1‑140 (MQ=255)                                  cTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCAc                                                                                                 >  2:1366492/1‑140 (MQ=255)                                     tCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGt                                                                                              >  1:1392314/1‑140 (MQ=255)                                     tCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGt                                                                                              >  1:1034763/1‑140 (MQ=255)                                     tCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGt                                                                                              >  1:316095/1‑140 (MQ=255)                                     tCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGt                                                                                              <  2:63596/140‑1 (MQ=255)                                     tCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGt                                                                                              >  2:661075/1‑140 (MQ=255)                                     tCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGt                                                                                              <  1:500885/140‑1 (MQ=255)                                       gggCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAg                                                                                            >  1:552345/1‑140 (MQ=255)                                       gggCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAg                                                                                            >  1:1045620/1‑140 (MQ=255)                                        ggCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGc                                                                                           >  1:89390/1‑140 (MQ=255)                                        ggCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGc                                                                                           <  1:759323/140‑1 (MQ=255)                                                   aCCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAgtgg                                                                                <  1:491953/140‑1 (MQ=255)                                                   aCCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAgtgg                                                                                >  2:929408/1‑140 (MQ=255)                                                   aCCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAgtgg                                                                                <  1:175920/140‑1 (MQ=255)                                                   aCCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAgtgg                                                                                <  2:1045620/140‑1 (MQ=255)                                                        gTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACg                                                                           >  2:864195/1‑140 (MQ=255)                                                         ttACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGc                                                                          >  2:892991/1‑140 (MQ=255)                                                               tGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGcgcg                                                                    <  1:206720/140‑1 (MQ=255)                                                               tGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGcgcg                                                                    <  2:1034763/140‑1 (MQ=255)                                                                 cGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCgcggc                                                                  >  1:384038/1‑140 (MQ=255)                                                                 cGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCgcggc                                                                  >  1:83032/1‑140 (MQ=255)                                                                    aaTTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGt                                                               <  2:565584/140‑1 (MQ=255)                                                                            aTCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCaa                                                       <  1:11073/140‑1 (MQ=255)                                                                                  gCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTgg                                                 <  2:316095/140‑1 (MQ=255)                                                                                      tACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCaaa                                             <  1:309112/140‑1 (MQ=255)                                                                                       aCTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAAt                                            <  2:728084/140‑1 (MQ=255)                                                                                        cTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATc                                           <  2:89390/140‑1 (MQ=255)                                                                                        cTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATc                                           <  1:5084/140‑1 (MQ=255)                                                                                        cTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATc                                           <  1:959062/140‑1 (MQ=255)                                                                                        cTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATc                                           <  1:1348968/140‑1 (MQ=255)                                                                                         ttCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCt                                          <  1:1366492/140‑1 (MQ=255)                                                                                          tCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCtt                                         <  1:317533/140‑1 (MQ=255)                                                                                             tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTTCt                                      >  2:1146100/1‑140 (MQ=255)                                                                                             tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCt                                      <  1:661075/140‑1 (MQ=255)                                                                                             tCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCt                                      >  1:218654/1‑140 (MQ=255)                                                                                              ccAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTa                                     <  2:218654/140‑1 (MQ=255)                                                                                                  tCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTa                                     >  2:87527/1‑136 (MQ=255)                                                                                                  tCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTa                                     <  1:87527/136‑1 (MQ=255)                                                                                                  tCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACaaa                                 >  1:553587/1‑140 (MQ=255)                                                                                                  tCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACaaa                                 >  1:843889/1‑140 (MQ=255)                                                                                                  tCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACaaa                                 >  1:62099/1‑140 (MQ=255)                                                                                                      gtgtATAGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTAt                             >  1:204090/1‑140 (MQ=255)                                                                                                          ataGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAg                         <  1:1392938/140‑1 (MQ=255)                                                                                                            aGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGa                       >  2:970243/1‑140 (MQ=255)                                                                                                            aGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGa                       >  1:1366567/1‑140 (MQ=255)                                                                                                            aGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGa                       >  1:440200/1‑140 (MQ=255)                                                                                                            aGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGa                       <  2:204090/140‑1 (MQ=255)                                                                                                            aGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGa                       <  2:1042135/140‑1 (MQ=255)                                                                                                            aGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAAAATGCCGGa                       >  1:555113/1‑140 (MQ=255)                                                                                                            aGCTATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGGTAAATTAATTTTGGCAAATCTTGCTACAAAGTATGCAGGa                       >  2:223915/1‑140 (MQ=255)                                                                                                               tATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAAtt                    <  2:62099/140‑1 (MQ=255)                                                                                                               tATTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAAtt                    <  1:518295/140‑1 (MQ=255)                                                                                                                aTTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTc                   <  1:1189765/140‑1 (MQ=255)                                                                                                                aTTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTc                   <  1:929408/140‑1 (MQ=255)                                                                                                                aTTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTc                   <  2:1392314/140‑1 (MQ=255)                                                                                                                aTTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTc                   >  1:633757/1‑140 (MQ=255)                                                                                                                aTTTTGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGGATGCAGGAATTc                   >  2:1004056/1‑140 (MQ=255)                                                                                                                 ttttGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCTCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCa                  >  1:1208203/1‑140 (MQ=255)                                                                                                                 ttttGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCa                  <  1:375587/140‑1 (MQ=255)                                                                                                                 ttttGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCa                  >  1:1187869/1‑140 (MQ=255)                                                                                                                 ttttGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCa                  <  1:162973/140‑1 (MQ=255)                                                                                                                 ttttGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCa                  <  2:225380/140‑1 (MQ=255)                                                                                                                   ttGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGAAGCCGTTTTAGTGGCGACGCAGCGCGGCGGTTTAATCAATTCTGGCGAAACTTGGTACAAAGGATGCGGGAAATCaca                >  2:914149/1‑139 (MQ=255)                                                                                                                    tGCCGTTGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCacta               <  1:585956/140‑1 (MQ=255)                                                                                                                         ttGCGCTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTa          >  2:1223583/1‑140 (MQ=255)                                                                                                                           gcgcTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTGAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACg        >  1:1189439/1‑140 (MQ=255)                                                                                                                            cgcTGGCGCAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGt       <  2:1187869/140‑1 (MQ=255)                                                                                                                                 gcgcAGGATGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGc  >  1:583172/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     AGCTGGCAGATATTCCCGTTCTTCATGCTTATCCTGTCGGGCAAAAAGATACCCCGTTACCGTGCGTAATTTTTTATCACGGCTTTACTTCATCCAGTCTGGTGTATAGCTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGC  >  NC_000913/4416974‑4417241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑