Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	845,139	G→T	F114L (TTC→TTA)	ybiO ←	moderate conductance mechanosensitive channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	845,139	0	G	T	100.0%	211.4 / NA	60	F114L (TTC→TTA)	ybiO	moderate conductance mechanosensitive channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (34/26);  total (34/26)

GTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGC  >  NC_000913/845003‑845274                                                                                                                                         |                                                                                                                                        gTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAg                                                                                                                                      >  1:1062108/1‑140 (MQ=255)    cgcACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAgcgg                                                                                                                                   >  1:1140233/1‑140 (MQ=255)     gcACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAgcggg                                                                                                                                  <  1:1141133/140‑1 (MQ=255)         aGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGa                                                                                                                              <  2:286654/140‑1 (MQ=255)            cGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAg                                                                                                                           <  2:171917/140‑1 (MQ=255)              aaTCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAggg                                                                                                                         >  2:1385519/1‑140 (MQ=255)              aaTCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAggg                                                                                                                         >  1:193870/1‑140 (MQ=255)               aTCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTTAGTGGGCGGCAAGGGc                                                                                                                        <  2:790602/140‑1 (MQ=255)               aTCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGc                                                                                                                        <  2:193870/140‑1 (MQ=255)               aTCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGc                                                                                                                        <  2:503344/140‑1 (MQ=255)                 cAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCtt                                                                                                                      <  1:566737/140‑1 (MQ=255)                 cAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCtt                                                                                                                      <  1:142536/140‑1 (MQ=255)                     cAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCtt                                                                                                                      >  2:1281414/1‑136 (MQ=255)                     cAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCtt                                                                                                                      <  1:1281414/136‑1 (MQ=255)                              ccAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATggcg                                                                                                         >  2:450789/1‑140 (MQ=255)                                        aTACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGCCTTATGCGGGGAGCCGGCGATATTGCGATAAATTTGCCCTAGGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCg                                                                                               >  2:471458/1‑140 (MQ=255)                                         tACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCgg                                                                                              >  1:445662/1‑140 (MQ=255)                                         tACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCgg                                                                                              <  1:1222998/140‑1 (MQ=255)                                           ccGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTg                                                                                            <  1:1385519/140‑1 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTg                                                                                            >  1:1431590/1‑137 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTg                                                                                            <  2:1431590/137‑1 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAcc                                                                                         >  2:657680/1‑140 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAcc                                                                                         >  2:92009/1‑140 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAcc                                                                                         >  1:765008/1‑140 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAcc                                                                                         >  2:216158/1‑140 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAcc                                                                                         >  2:334702/1‑140 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAcc                                                                                         <  1:450789/140‑1 (MQ=255)                                              cTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGAcc                                                                                         >  2:415087/1‑140 (MQ=255)                                                aaCATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGGTAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCtt                                                                                       <  2:1364505/140‑1 (MQ=255)                                                       aaaaaTGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTgcagc                                                                                <  1:670611/140‑1 (MQ=255)                                                        aaaaTGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTgcagca                                                                               <  1:415087/140‑1 (MQ=255)                                                         aaaTGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCAc                                                                              <  1:743476/140‑1 (MQ=255)                                                            tGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGt                                                                           >  1:186599/1‑140 (MQ=255)                                                              ggTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGttt                                                                         <  2:1062108/140‑1 (MQ=255)                                                              ggTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGttt                                                                         <  1:657680/140‑1 (MQ=255)                                                               gTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTg                                                                        >  2:1229073/1‑140 (MQ=255)                                                                 cAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGct                                                                      >  2:1296221/1‑140 (MQ=255)                                                                        ttGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGAc                                                               >  1:442971/1‑140 (MQ=255)                                                                        ttGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGAc                                                               >  1:80311/1‑140 (MQ=255)                                                                         tGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGAcc                                                              >  1:1037735/1‑140 (MQ=255)                                                                              aaGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGt                                                         >  2:1245404/1‑140 (MQ=255)                                                                                   ttGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTcggcg                                                    >  1:1104989/1‑140 (MQ=255)                                                                                   ttGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTcggcg                                                    >  1:515786/1‑140 (MQ=255)                                                                                    tgtgGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTcggcgg                                                   >  2:801476/1‑140 (MQ=255)                                                                                    tgtgGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTcggcgg                                                   <  2:1403857/140‑1 (MQ=255)                                                                                    tgtgGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTcggcgg                                                   >  1:1403857/1‑140 (MQ=255)                                                                                     gtgGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCggcggc                                                  <  2:186599/140‑1 (MQ=255)                                                                                     gtgGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCggcggc                                                  >  1:399053/1‑140 (MQ=255)                                                                                        gATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACt                                               <  2:63154/140‑1 (MQ=255)                                                                                           tAAACGGCTTATGCGGGGAGCTGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTAtt                                            >  1:332277/1‑140 (MQ=255)                                                                                           tAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTAtt                                            >  1:137018/1‑140 (MQ=255)                                                                                                ggCTTATGCGGGGAGCTGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTgg                                       <  2:332277/140‑1 (MQ=255)                                                                                                   ttATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTAc                                    <  2:137018/140‑1 (MQ=255)                                                                                                        cGGGGAGCCGGTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGtt                               <  2:765008/140‑1 (MQ=255)                                                                                                                 ggTGATATTGCGATAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAgggg                      >  1:727676/1‑140 (MQ=255)                                                                                                                             aTAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCCGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCaa          >  2:471153/1‑140 (MQ=255)                                                                                                                             aTAAAGTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCA‑GGGGTGTAGCGGCAAc         >  1:133705/1‑140 (MQ=255)                                                                                                                                  gTTGCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTg     <  2:1104989/140‑1 (MQ=255)                                                                                                                                     gCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCaa          >  1:944378/1‑132 (MQ=255)                                                                                                                                     gCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCaa          <  2:944378/132‑1 (MQ=255)                                                                                                                                     gCCCTAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTgcgc  >  2:1096313/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                        GTGCGCACAGGCGAATCAGCCAGTAAAAACCAAACACTAATACCGCTAACATTGAAAAATGGGTCAGCGCATTGCTGAAGGTTTGTGGATTAAACGGCTTATGCGGGGAGCCGGTGATATTGCGATAAAGTTGCCCGAAGCGGGCGGAAAGGGCTTCACCATAATGGCGGCTGACTTCGGTGACCTTTTGCAGCACGGTTTGCTCTTCGACCAGCGTCGGCGGCACTATTTTTGGTACCGGTTCAGCAGGGGGCGTAGCGGCAACGGTGCGC  >  NC_000913/845003‑845274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑