Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,653,325	T→C	E197E (GAA→GAG)	rspB ←	putative zinc‑binding dehydrogenase RspB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,653,325	0	T	C	100.0%	271.8 / NA	72	E197E (GAA→GAG)	rspB	putative zinc‑binding dehydrogenase RspB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (31/41);  total (31/41)

TCAGGATAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGTTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATCATTTTCAGTCGGTTGACCATGTC  >  NC_000913/1653189‑1653455                                                                                                                                         |                                                                                                                                   tCAGGATAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCtca                                                                                                                                 <  1:537535/140‑1 (MQ=255) tCAGGATAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCtca                                                                                                                                 >  1:855427/1‑140 (MQ=255)   aGGATAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCtcatc                                                                                                                               <  1:460945/140‑1 (MQ=255)   aGGATAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCtcatc                                                                                                                               <  1:66067/140‑1 (MQ=255)     gATAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCaa                                                                                                                             >  2:1226942/1‑140 (MQ=255)       tAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATg                                                                                                                           <  1:787797/140‑1 (MQ=255)       tAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATg                                                                                                                           <  1:1406190/140‑1 (MQ=255)       tAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATg                                                                                                                           <  1:1131524/140‑1 (MQ=255)         gAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGGAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCg                                                                                                                         >  1:708948/1‑140 (MQ=255)         gAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCg                                                                                                                         <  2:1121428/140‑1 (MQ=255)         gAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCg                                                                                                                         >  2:136476/1‑140 (MQ=255)         gAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCg                                                                                                                         <  1:226098/140‑1 (MQ=255)           aGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGAt                                                                                                                       <  2:235526/140‑1 (MQ=255)               tGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGc                                                                                                                   <  1:1181905/140‑1 (MQ=255)               tGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGc                                                                                                                   >  2:1174755/1‑140 (MQ=255)               tGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGc                                                                                                                   >  1:277692/1‑140 (MQ=255)                 aCAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGcaa                                                                                                                 <  1:413470/140‑1 (MQ=255)                   aaGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGcaaca                                                                                                               <  1:974373/140‑1 (MQ=255)                     gCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAAt                                                                                                             >  2:1431856/1‑140 (MQ=255)                         cATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATcaca                                                                                                         >  1:1437016/1‑140 (MQ=255)                                       gTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTtata                                                                                           >  2:804709/1‑140 (MQ=255)                                        tCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAATCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAg                                                                                          >  2:581271/1‑140 (MQ=255)                                         cGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGa                                                                                         <  1:1431856/140‑1 (MQ=255)                                         cGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGa                                                                                         <  1:1408722/140‑1 (MQ=255)                                          ggCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACAtt                                                                                                       >  2:65968/1‑125 (MQ=255)                                          ggCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACAtt                                                                                                       <  1:65968/125‑1 (MQ=255)                                           gCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACg                                                                                       >  1:232808/1‑140 (MQ=255)                                           gCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACg                                                                                       <  2:664445/140‑1 (MQ=255)                                           gCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACg                                                                                       >  1:16018/1‑140 (MQ=255)                                            cTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAATAATCACATTTTTAACGTTATAGACGc                                                                                      <  2:708948/140‑1 (MQ=255)                                            cTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGc                                                                                      <  2:558435/140‑1 (MQ=255)                                            cTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGc                                                                                      <  1:662792/140‑1 (MQ=255)                                            cTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGc                                                                                      <  1:804709/140‑1 (MQ=255)                                            cTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGc                                                                                      >  2:755222/1‑140 (MQ=255)                                            cTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGc                                                                                      <  2:896389/140‑1 (MQ=255)                                            cTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGc                                                                                      <  1:1071313/140‑1 (MQ=255)                                                aTGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCttt                                                                                  >  1:1081819/1‑140 (MQ=255)                                                aTGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCttt                                                                                  >  1:926308/1‑140 (MQ=255)                                                 tGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCtttt                                                                                 >  1:921968/1‑140 (MQ=255)                                                        ttCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCt                                                                          <  1:1396446/140‑1 (MQ=255)                                                             tGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACg                                                                     >  2:1152589/1‑140 (MQ=255)                                                                           aGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGAt                                                       <  2:449268/140‑1 (MQ=255)                                                                                   cTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCgg                                               <  2:926308/140‑1 (MQ=255)                                                                                   cTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCgg                                               <  1:958836/140‑1 (MQ=255)                                                                                   cTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCgg                                               <  2:855427/140‑1 (MQ=255)                                                                                        tGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCa                                          <  1:136476/140‑1 (MQ=255)                                                                                        tGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCa                                          <  2:1137740/140‑1 (MQ=255)                                                                                        tGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCa                                          <  1:773377/140‑1 (MQ=255)                                                                                        tGTTATTAATCGCCCAGGCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCa                                          <  2:1037125/140‑1 (MQ=255)                                                                                          ttattaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCa                                          >  1:769768/1‑138 (MQ=255)                                                                                          ttattaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCa                                          <  2:769768/138‑1 (MQ=255)                                                                                          ttattaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATa                                        >  1:474569/1‑140 (MQ=255)                                                                                          ttattaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATa                                        >  2:770409/1‑140 (MQ=255)                                                                                          ttattaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATa                                        <  1:672207/140‑1 (MQ=255)                                                                                          ttattaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATa                                        >  2:562154/1‑140 (MQ=255)                                                                                          ttattaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATa                                        >  1:346307/1‑140 (MQ=255)                                                                                            attaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATaaa                                      >  2:1185677/1‑140 (MQ=255)                                                                                            attaATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATaaa                                      >  1:1155976/1‑140 (MQ=255)                                                                                                aTCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAg                                  <  1:1174755/140‑1 (MQ=255)                                                                                                 tCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGa                                 >  1:29716/1‑140 (MQ=255)                                                                                                  cGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGaa                                >  1:206154/1‑140 (MQ=255)                                                                                                    cccAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACg                              >  2:560593/1‑140 (MQ=255)                                                                                                    cccAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACg                              >  1:34146/1‑140 (MQ=255)                                                                                                    cccAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACg                              <  2:277692/140‑1 (MQ=255)                                                                                                    cccAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACg                              >  2:219415/1‑140 (MQ=255)                                                                                                         tCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATc                         >  2:363482/1‑140 (MQ=255)                                                                                                                cGCTCTCTTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATCATTTTCa                  <  2:1081819/140‑1 (MQ=255)                                                                                                                      ctTTCGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATCATTTTCAGTCGGt            <  1:560593/140‑1 (MQ=255)                                                                                                                          cGCTTTTTCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATCATTTTCAGTCGGTTGAc        <  2:159300/140‑1 (MQ=255)                                                                                                                             tttttCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATCATTTTCAGTCGGTTGACCAt     <  2:474569/140‑1 (MQ=255)                                                                                                                              ttttCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATCATTTTCAGTCGGTTGACCATg    <  2:550611/140‑1 (MQ=255)                                                                                                                                ttCCAGTCGCTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATCATTTTCAGTCGGTTGACCATGTc  <  2:206154/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                   TCAGGATAGAAGGATGACAAGCCGCATCGATAATTAATGTCGGCTTGATGCCTTTTTCAGTGAAAATCTCGCCAAGCGGTGTCTGGCTGTTATTAATCGCCCAGTCAGCCCCGCTCTCTTTCGCTTTTTCCAGTCGTTCATCAATGCGATCGGCAACAATCACATTTTTAACGTTATAGACGCCTTTTAATACCTGAACGATCGTCAGGCCGATTGGACCGGCACCATAAACCAGAACGGTATCATTTTCAGTCGGTTGACCATGTC  >  NC_000913/1653189‑1653455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑