Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,004,386	T→C	G467G (GGA→GGG)	ygeV ←	putative sigma(54)‑dependent transcriptional regulator YgeV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,004,386	0	T	C	100.0%	291.2 / NA	77	G467G (GGA→GGG)	ygeV	putative sigma(54)‑dependent transcriptional regulator YgeV
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/45);  total (32/45)

GTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTT  >  NC_000913/3004249‑3004519                                                                                                                                          |                                                                                                                                      gTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAGCTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGAccc                                                                                                                                     <  2:841595/140‑1 (MQ=255)    cTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAg                                                                                                                                  >  2:345999/1‑140 (MQ=255)      gTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAgac                                                                                                                                <  2:934671/140‑1 (MQ=255)       tCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAgacg                                                                                                                               <  1:545314/140‑1 (MQ=255)        cGATAACTTCGCCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAgacga                                                                                                                              <  2:157525/140‑1 (MQ=255)        cGATAACTTCACCTGAAGGAACCACGTTAACCAGTTATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAgacga                                                                                                                              <  1:398998/140‑1 (MQ=255)        cGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAgacga                                                                                                                              <  2:231113/140‑1 (MQ=255)        cGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAgacga                                                                                                                              <  1:177568/140‑1 (MQ=255)        cGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAgacga                                                                                                                              <  1:810872/140‑1 (MQ=255)        cGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAgacga                                                                                                                              <  1:335131/140‑1 (MQ=255)              cTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTa                                                                                                                        >  2:789997/1‑140 (MQ=255)                tCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATg                                                                                                                      <  1:605995/140‑1 (MQ=255)                 cACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGc                                                                                                                     >  2:1145743/1‑140 (MQ=255)                 cACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGc                                                                                                                     >  1:476191/1‑140 (MQ=255)                 cACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGc                                                                                                                     >  1:897458/1‑140 (MQ=255)                  aCCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCa                                                                                                                    <  2:897458/140‑1 (MQ=255)                   ccTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAg                                                                                                                   <  2:140289/140‑1 (MQ=255)                   ccTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAg                                                                                                                   <  2:930606/140‑1 (MQ=255)                   ccTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACAACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGTAg                                                                                                                   >  2:421452/1‑140 (MQ=255)                     tGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGAt                                                                                                                 >  1:726169/1‑140 (MQ=255)                        aGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTa                                                                                                              <  2:726169/140‑1 (MQ=255)                              cACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGt                                                                                                        <  1:345999/140‑1 (MQ=255)                              cACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGt                                                                                                        <  2:988403/140‑1 (MQ=255)                              cACGTTAACCAGACATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGt                                                                                                        >  1:457651/1‑140 (MQ=255)                                cGTTAACCAGATATTCCATCATATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTaa                                                                                                      <  1:594800/140‑1 (MQ=255)                                 gTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGCTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAAt                                                                                                     >  2:460722/1‑140 (MQ=255)                                  ttAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATg                                                                                                    <  2:105130/140‑1 (MQ=255)                                  ttAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATg                                                                                                    <  2:91788/140‑1 (MQ=255)                                     aCCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCAc                                                                                                 >  2:851552/1‑140 (MQ=255)                                      ccAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCAcc                                                                                                <  1:144498/140‑1 (MQ=255)                                        aGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCaa                                                                                              <  2:313206/140‑1 (MQ=255)                                        aGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCaa                                                                                              <  1:1145743/140‑1 (MQ=255)                                        aGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCaa                                                                                              <  1:719122/140‑1 (MQ=255)                                        aGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCaa                                                                                              <  1:661757/140‑1 (MQ=255)                                          atatTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAAt                                                                                                     >  2:622073/1‑131 (MQ=255)                                          atatTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAAt                                                                                                     <  1:622073/131‑1 (MQ=255)                                            atTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTACGACGAAATCGGGAGCAATGACAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGt                                                                                          <  2:132439/140‑1 (MQ=255)                                             ttCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGtt                                                                                         <  1:795958/140‑1 (MQ=255)                                              tCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAAt                                                                                             >  2:974719/1‑135 (MQ=255)                                              tCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAAt                                                                                             <  1:974719/135‑1 (MQ=255)                                               ccATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCa                                                                                       >  1:973501/1‑140 (MQ=255)                                                  tCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAata                                                                                    <  1:851552/140‑1 (MQ=255)                                                   cAAATTGCTTAACTCGCGCAGGTTTCCGGGCAAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAatat                                                                                   >  2:964619/1‑140 (MQ=255)                                                    aaaTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATc                                                                                  >  1:413785/1‑140 (MQ=255)                                                      aTTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCtt                                                                                >  1:1133176/1‑140 (MQ=255)                                                        tGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTcc                                                                              >  1:835093/1‑140 (MQ=255)                                                          cTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTg                                                                            >  2:993463/1‑140 (MQ=255)                                                               cTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGtt                                                                       <  1:626205/140‑1 (MQ=255)                                                                      aGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAg                                                                <  2:306480/140‑1 (MQ=255)                                                                         tttCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCgg                                                             >  1:1007445/1‑140 (MQ=255)                                                                          ttCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAg                                                                <  1:713221/136‑1 (MQ=255)                                                                          ttCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAg                                                                >  2:713221/1‑136 (MQ=255)                                                                            ccGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTgg                                                          <  1:944113/140‑1 (MQ=255)                                                                             cGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGc                                                         >  1:275266/1‑140 (MQ=255)                                                                              gggCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCa                                                        <  2:275266/140‑1 (MQ=255)                                                                              gggCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCa                                                        <  2:797979/140‑1 (MQ=255)                                                                              gggCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCa                                                        <  1:816028/140‑1 (MQ=255)                                                                                  cAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGt                                                    >  1:880134/1‑140 (MQ=255)                                                                                     cGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTAt                                                 <  2:1110706/140‑1 (MQ=255)                                                                                         gCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCggg                                             <  2:476191/140‑1 (MQ=255)                                                                                         gCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCggg                                             <  2:1138935/140‑1 (MQ=255)                                                                                         gCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCggg                                             <  2:635578/140‑1 (MQ=255)                                                                                          cTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGa                                            <  1:993463/140‑1 (MQ=255)                                                                                          cTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGa                                            >  1:536413/1‑140 (MQ=255)                                                                                           ttACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGAt                                           <  2:457651/140‑1 (MQ=255)                                                                                                   tatTTCGACGACATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTaa                                   <  2:635337/140‑1 (MQ=255)                                                                                                        cgacgaCATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCgg                              <  2:282388/140‑1 (MQ=255)                                                                                                        cgacgaCATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCgg                              <  2:413785/140‑1 (MQ=255)                                                                                                           cgacATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGGAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTag                           >  2:495780/1‑140 (MQ=255)                                                                                                            gacATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTaga                          >  2:589951/1‑140 (MQ=255)                                                                                                             acATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTagaa                         >  2:1121414/1‑140 (MQ=255)                                                                                                             acATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTagaa                         >  1:583767/1‑140 (MQ=255)                                                                                                             acATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTagaa                         >  2:911477/1‑140 (MQ=255)                                                                                                             acATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTagaa                         >  2:190292/1‑140 (MQ=255)                                                                                                              cATCGGGAGCAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTagaag                        >  2:227214/1‑140 (MQ=255)                                                                                                                       cAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTcg               >  2:1000387/1‑140 (MQ=255)                                                                                                                       cAATGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTcg               <  1:190292/140‑1 (MQ=255)                                                                                                                          tGCCAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCgg            >  1:462362/1‑140 (MQ=255)                                                                                                                             cAGGATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGACGGTAGAAGAGATCTTCGCGGAAt         <  1:495780/140‑1 (MQ=255)                                                                                                                               ggATAAACCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAAttt       >  2:495781/1‑140 (MQ=255)                                                                                                                                    aaCCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCtt  <  1:227214/140‑1 (MQ=255)                                                                                                                                    aaCCGACCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCtt  <  1:12025/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      GTGCTGTCGATAACTTCACCTGAAGGAACCACGTTAACCAGATATTCCATCAAATTGCTTAACTCGCGCAGGTTTCCGGGCCAACGATGCTTACGCAATATTTCGACGACATCGGGAGCAATGCCAGGATAAACCGATCCCAGACGACGGGTATGCAGATGTAAAAAGTAATGCACCAATAGTTCAATATCTTCCTGACGTTCACGCAGCGGTGGCAGAGTTATCGGGATAACATTAAGTCGGTAGAAGAGATCTTCGCGGAATTTACCTT  >  NC_000913/3004249‑3004519

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑