Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,878,717	G→T	A468E (GCG→GAG)	gyrB ←	DNA gyrase subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,878,717	0	G	T	100.0%	302.9 / NA	86	A468E (GCG→GAG)	gyrB	DNA gyrase subunit B
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/47);  total (39/47)

AAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCA  >  NC_000913/3878582‑3878852                                                                                                                                        |                                                                                                                                        aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGTCCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCAc                                                                                                                                     <  2:40833/140‑1 (MQ=255) aagGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCAc                                                                                                                                     <  2:974830/140‑1 (MQ=255)   ggTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACtt                                                                                                                                   >  1:563584/1‑140 (MQ=255)   ggTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACtt                                                                                                                                   >  2:380997/1‑140 (MQ=255)     tCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTcc                                                                                                                                 >  2:607198/1‑140 (MQ=255)     tCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTcc                                                                                                                                 >  1:193511/1‑140 (MQ=255)           gcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGagaa                                                                                                                           <  2:683356/140‑1 (MQ=255)           gcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGagaa                                                                                                                           <  1:425803/140‑1 (MQ=255)           gcagcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGagaa                                                                                                                           <  2:1017734/140‑1 (MQ=255)              gcGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAagag                                                                                                                        >  1:150659/1‑140 (MQ=255)                gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAagagag                                                                                                                      <  1:988847/140‑1 (MQ=255)                gTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAagagag                                                                                                                      <  2:43661/140‑1 (MQ=255)                  aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCa                                                                                                                    <  1:380997/140‑1 (MQ=255)                  aCGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCa                                                                                                                    <  2:193511/140‑1 (MQ=255)                   cGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCAt                                                                                                                   <  1:157113/140‑1 (MQ=255)                   cGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCAt                                                                                                                   <  1:692089/140‑1 (MQ=255)                       tgtgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTa                                                                                                               <  1:887812/140‑1 (MQ=255)                       tgtgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTa                                                                                                               <  1:607198/140‑1 (MQ=255)                         tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATc                                                                                                             >  1:906273/1‑140 (MQ=255)                         tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATc                                                                                                             >  1:6828/1‑140 (MQ=255)                         tgCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATc                                                                                                             >  1:1038120/1‑140 (MQ=255)                           cGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGa                                                                                                           >  2:115759/1‑140 (MQ=255)                               ccGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcg                                                                                                       >  2:208533/1‑140 (MQ=255)                               ccGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcg                                                                                                       <  1:983903/140‑1 (MQ=255)                                 gTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcg                                                                                                     <  1:300342/140‑1 (MQ=255)                                 gTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcg                                                                                                     <  1:855814/140‑1 (MQ=255)                                  tCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAgcgcgc                                                                                                    >  2:57224/1‑140 (MQ=255)                                          cGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGa                                                                                            >  2:638732/1‑140 (MQ=255)                                            cATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACg                                                                                          >  2:818140/1‑140 (MQ=255)                                              tCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                                                                                        <  2:563584/140‑1 (MQ=255)                                              tCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGtt                                                                                        <  1:779132/140‑1 (MQ=255)                                               cGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTg                                                                                       <  1:818140/140‑1 (MQ=255)                                                ggTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa                                                                                      <  1:1067264/140‑1 (MQ=255)                                                ggTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGa                                                                                      <  2:104531/140‑1 (MQ=255)                                                 gTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAg                                                                                     >  1:241589/1‑140 (MQ=255)                                                         gatgatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAc                                                                             <  2:100735/140‑1 (MQ=255)                                                           tgatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc                                                                           >  2:215772/1‑140 (MQ=255)                                                           tgatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTAccc                                                                           >  1:41645/1‑140 (MQ=255)                                                            gatgCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCt                                                                          >  1:846448/1‑140 (MQ=255)                                                                   tgATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg                                                                   >  1:887637/1‑140 (MQ=255)                                                                   tgATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCgg                                                                   >  2:405598/1‑140 (MQ=255)                                                                      tAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAg                                                                <  1:405598/140‑1 (MQ=255)                                                                       aaCGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGa                                                               <  2:877662/140‑1 (MQ=255)                                                                        aCGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGaa                                                              <  2:906273/140‑1 (MQ=255)                                                                             gTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGcc                                                         <  2:887637/140‑1 (MQ=255)                                                                               ttGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTg                                                       >  2:791146/1‑140 (MQ=255)                                                                               ttGTCCGGGTTGCACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGCCTCCAATTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTg                                                       >  2:370675/1‑140 (MQ=255)                                                                                  tCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGtt                                                    >  2:1117232/1‑140 (MQ=255)                                                                                    cGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCt                                                  >  2:345321/1‑140 (MQ=255)                                                                                     gggTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCtt                                                 <  2:1038120/140‑1 (MQ=255)                                                                                      ggTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTg                                                <  2:846448/140‑1 (MQ=255)                                                                                       gTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGc                                               >  2:842005/1‑140 (MQ=255)                                                                                          gTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGt                                            <  2:515283/140‑1 (MQ=255)                                                                                           tACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGtt                                           >  1:100697/1‑140 (MQ=255)                                                                                            aCTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTa                                          <  2:304702/140‑1 (MQ=255)                                                                                            aCTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTa                                          <  1:357995/140‑1 (MQ=255)                                                                                            aCTCGTCACGACCGATACCACAGCCAAGCGCGGCGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTa                                          >  2:935619/1‑140 (MQ=255)                                                                                                gTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCt                                                  >  1:1120849/1‑128 (MQ=255)                                                                                                gTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGcc                                      >  2:687236/1‑140 (MQ=255)                                                                                                gTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTACCCTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCt                                                  <  2:1120849/128‑1 (MQ=255)                                                                                                 tCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGccc                                     <  1:687236/140‑1 (MQ=255)                                                                                                          aTACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGc                                       <  1:1125025/129‑1 (MQ=255)                                                                                                          aTACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGc                                       >  2:1125025/1‑129 (MQ=255)                                                                                                          aTACCACAGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGc                            >  2:1110276/1‑140 (MQ=255)                                                                                                               acaGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAgc                       <  2:241589/140‑1 (MQ=255)                                                                                                                 aGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTg                                  <  1:599761/127‑1 (MQ=255)                                                                                                                 aGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTg                                  >  2:599761/1‑127 (MQ=255)                                                                                                                 aGCCAAGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGA‑TTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAgccgc                    >  2:467164/1‑140 (MQ=255)                                                                                                                     aaGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGccgcccg                 >  2:1036654/1‑140 (MQ=255)                                                                                                                     aaGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGccgcccg                 >  1:969964/1‑140 (MQ=255)                                                                                                                      aGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGccgcccgc                >  2:485981/1‑140 (MQ=255)                                                                                                                      aGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGccgcccgc                >  1:305070/1‑140 (MQ=255)                                                                                                                      aGCGCGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGccgcccgc                >  1:672042/1‑140 (MQ=255)                                                                                                                       gcgcGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCcgcg               >  2:449588/1‑140 (MQ=255)                                                                                                                       gcgcGGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCcgcg               <  1:1110276/140‑1 (MQ=255)                                                                                                                        cgcgGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCcgcg               >  2:1094601/1‑139 (MQ=255)                                                                                                                        cgcgGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCcgcg               <  1:1094601/139‑1 (MQ=255)                                                                                                                          cgGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAgccgc                    <  2:88084/132‑1 (MQ=255)                                                                                                                          cgGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAgccgc                    >  1:88084/1‑132 (MQ=255)                                                                                                                          cgGTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAg            <  2:6828/140‑1 (MQ=255)                                                                                                                           ggTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGt           <  1:842005/140‑1 (MQ=255)                                                                                                                           ggTGATAAGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGt           <  2:41645/140‑1 (MQ=255)                                                                                                                                 aaGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCtt     <  1:485981/140‑1 (MQ=255)                                                                                                                                 aaGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCtt     <  1:215772/140‑1 (MQ=255)                                                                                                                                  aGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCcgcg               <  1:506143/129‑1 (MQ=255)                                                                                                                                  aGCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCcgcg               >  2:506143/1‑129 (MQ=255)                                                                                                                                   gCGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTcc   <  2:175810/140‑1 (MQ=255)                                                                                                                                    cGTCTCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTcca  <  1:1117232/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        AAGGTCAACAGCAGCGTACGAATGTGCGAGCCGTCGACGTCCGCATCGGTCATGATGATGATGCTGTGATAACGCAGTTTGTCCGGGTTGTACTCGTCACGACCGATACCACAGCCAAGCGCGGTGATAAGCGTCGCCACTTCCTGAGAAGAGAGCATCTTATCGAAGCGCGCTTTCTCGACGTTGAGGATTTTACCCTTCAGCGGCAGAATCGCCTGGTTCTTGCGGTTACGCCCCTGCTTCGCAGAGCCGCCCGCGGAGTCCCCTTCCA  >  NC_000913/3878582‑3878852

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑