Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	799,274	G→T	W230L (TGG→TTG)	pgl →	6‑phosphogluconolactonase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	799,274	0	G	T	100.0%	254.2 / NA	72	W230L (TGG→TTG)	pgl	6‑phosphogluconolactonase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/36);  total (36/36)

TCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTGGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCGTGTTGAGTAAAGAAGGCTTCCAGCCAAC  >  NC_000913/799137‑799403                                                                                                                                          |                                                                                                                                  tccaAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTtgg                                                                                                                                 >  1:579593/1‑140 (MQ=255) tccaAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTtgg                                                                                                                                 >  2:417200/1‑140 (MQ=255) tccaAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTtgg                                                                                                                                 >  1:649831/1‑140 (MQ=255) tccaAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTtgg                                                                                                                                 >  1:120328/1‑140 (MQ=255)    aaaCGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTggcgg                                                                                                                              <  2:1060611/140‑1 (MQ=255)    aaaCGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTggcgg                                                                                                                              >  2:232852/1‑140 (MQ=255)    aaaCGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGG‑AAACTTCTCCGACACCCGTTTggcggc                                                                                                                             <  1:660605/140‑1 (MQ=255)     aaCGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTggcggc                                                                                                                             <  1:775454/140‑1 (MQ=255)      aCGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCt                                                                                                                            <  1:561093/140‑1 (MQ=255)           cAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGatat                                                                                                                       <  2:120328/140‑1 (MQ=255)           cAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGatat                                                                                                                       <  1:969744/140‑1 (MQ=255)             atatGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTggcggc                                                                                                                             >  2:462996/1‑132 (MQ=255)             atatGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTggcggc                                                                                                                             <  1:462996/132‑1 (MQ=255)                  cGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATc                                                                                                                >  1:988041/1‑140 (MQ=255)                      ttGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCAccc                                                                                                            >  2:7279/1‑140 (MQ=255)                      ttGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCAccc                                                                                                            >  2:710348/1‑140 (MQ=255)                         cGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCgg                                                                                                         >  2:453707/1‑140 (MQ=255)                           tCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGAt                                                                                                       >  2:1105416/1‑140 (MQ=255)                            cAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATg                                                                                                      >  2:466374/1‑140 (MQ=255)                            cAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATg                                                                                                      >  1:169482/1‑140 (MQ=255)                             aaTGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATgg                                                                                                     >  1:1147092/1‑140 (MQ=255)                              aTGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGt                                                                                                    <  2:660661/140‑1 (MQ=255)                               tGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTc                                                                                                   <  2:649831/140‑1 (MQ=255)                                 aGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGc                                                                                                 <  1:417200/140‑1 (MQ=255)                                  gTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGcc                                                                                                <  2:37054/140‑1 (MQ=255)                                           cTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGcc                                                                                                >  1:264133/1‑131 (MQ=255)                                           cTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGcc                                                                                                <  2:264133/131‑1 (MQ=255)                                           cTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACg                                                                                       >  1:9715/1‑140 (MQ=255)                                           cTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACg                                                                                       >  1:291967/1‑140 (MQ=255)                                             cAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGcc                                                                                     <  1:410398/140‑1 (MQ=255)                                             cAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGcc                                                                                     >  1:348523/1‑140 (MQ=255)                                                tGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGc                                                                                  >  2:95037/1‑140 (MQ=255)                                                tGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGc                                                                                  >  1:1108421/1‑140 (MQ=255)                                                   aTGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGAc                                                                               >  1:87695/1‑140 (MQ=255)                                                     gTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCg                                                                             <  2:703208/140‑1 (MQ=255)                                                     gTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCg                                                                             >  1:634284/1‑140 (MQ=255)                                                     gTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCg                                                                             <  1:453707/140‑1 (MQ=255)                                                     gTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCg                                                                             <  1:1105416/140‑1 (MQ=255)                                                          ggAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCg                                                                        >  2:211299/1‑140 (MQ=255)                                                          ggAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCg                                                                        >  1:555120/1‑140 (MQ=255)                                                            aaCTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGcc                                                                      <  1:330288/140‑1 (MQ=255)                                                              cTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAg                                                                    <  1:1098532/140‑1 (MQ=255)                                                              cTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAg                                                                    <  2:555120/140‑1 (MQ=255)                                                                 aaaGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCt                                                                 >  2:787858/1‑140 (MQ=255)                                                                 aaaGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCt                                                                 >  1:214498/1‑140 (MQ=255)                                                                 aaaGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCt                                                                 >  2:700070/1‑140 (MQ=255)                                                                   aGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGa                                                               <  1:95037/140‑1 (MQ=255)                                                                               tAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCa                                                   <  1:466374/140‑1 (MQ=255)                                                                               tAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCa                                                   >  2:55827/1‑140 (MQ=255)                                                                                aaTATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAg                                                  <  2:634284/140‑1 (MQ=255)                                                                                     cGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGttt                                             <  2:988041/140‑1 (MQ=255)                                                                                     cGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGttt                                             <  2:291967/140‑1 (MQ=255)                                                                                       aaTGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCg                                           >  1:964263/1‑140 (MQ=255)                                                                                       aaTGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCg                                           >  1:611927/1‑140 (MQ=255)                                                                                         tgtgTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGttt                                             <  2:432963/136‑1 (MQ=255)                                                                                         tgtgTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGttt                                             >  1:432963/1‑136 (MQ=255)                                                                                         tgtgTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGttt                                             <  2:545151/136‑1 (MQ=255)                                                                                         tgtgTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGttt                                             >  1:545151/1‑136 (MQ=255)                                                                                         tgtgTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGa                                         <  1:55827/140‑1 (MQ=255)                                                                                         tgtgTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGa                                         >  1:952940/1‑140 (MQ=255)                                                                                         tgtgTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGa                                         >  2:757060/1‑140 (MQ=255)                                                                                         tgtgTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGa                                         <  2:9715/140‑1 (MQ=255)                                                                                             tCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGAt                                     >  1:1054061/1‑140 (MQ=255)                                                                                                 gACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCa                                 >  2:483469/1‑140 (MQ=255)                                                                                                 gACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCa                                 <  1:538979/140‑1 (MQ=255)                                                                                                     cTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCgt                             <  1:211299/140‑1 (MQ=255)                                                                                                      tGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCgtg                            <  1:710348/140‑1 (MQ=255)                                                                                                      tGGATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCgtg                            <  1:349660/140‑1 (MQ=255)                                                                                                       ggATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCgtg                            >  2:641376/1‑139 (MQ=255)                                                                                                       ggATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCgtg                            <  1:641376/139‑1 (MQ=255)                                                                                                        gATATGATGCCGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCGTGtt                          <  2:611927/140‑1 (MQ=255)                                                                                                                  cGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGc                               <  1:879447/125‑1 (MQ=255)                                                                                                                  cGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGc                               >  2:879447/1‑125 (MQ=255)                                                                                                                  cGGAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCGTGTTGAGTAaagaa                >  2:1009007/1‑140 (MQ=255)                                                                                                                    gAAAACTTCTCCGACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCGTGTTGAGTAAAGAAgg              >  2:1027365/1‑140 (MQ=255)                                                                                                                                gACACCCGTTTGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCGTGTTGAGTAAAGAAGGCTTCCAGCCAAc  <  2:824419/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                  TCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTGGATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATGATGCCGGAAAACTTCTCCGACACCCGTTGGGCGGCTGATATTCATATCACCCCGGATGGTCGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAAGATGGCAGCGTGTTGAGTAAAGAAGGCTTCCAGCCAAC  >  NC_000913/799137‑799403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑