Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,676,788	C→A	P372P (CCG→CCT)	pntA ←	pyridine nucleotide transhydrogenase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,676,788	0	C	A	100.0%	179.7 / NA	51	P372P (CCG→CCT)	pntA	pyridine nucleotide transhydrogenase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/27);  total (24/27)

GCCATCCAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATCGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGATTAACGAGGTTTGTGCCG  >  NC_000913/1676654‑1676923                                                                                                                                       |                                                                                                                                        gCCATCCAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATaggcgg                                                                                                                                    >  2:427153/1‑140 (MQ=255)  ccATCCAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGt                                                                                                                                   <  1:245020/140‑1 (MQ=255)     tCCAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGcc                                                                                                                                <  1:750100/140‑1 (MQ=255)      ccAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCg                                                                                                                               <  2:417789/140‑1 (MQ=255)         gCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCgg                                                                                                                              <  1:846091/138‑1 (MQ=255)         gCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCgg                                                                                                                              >  2:846091/1‑138 (MQ=255)                    tGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCg                                                                                                                 >  1:573024/1‑140 (MQ=255)                    tGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCg                                                                                                                 <  1:252224/140‑1 (MQ=255)                      aTTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGcc                                                                                                               >  1:1000393/1‑140 (MQ=255)                         gCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGc                                                                                                            >  1:306167/1‑140 (MQ=255)                          ccAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCa                                                                                                           <  2:1088595/140‑1 (MQ=255)                            aGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACg                                                                                                         >  2:988290/1‑140 (MQ=255)                                 cATCAACGCGTA‑TTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCAc                                                                                                   >  2:1144999/1‑140 (MQ=255)                                         cgTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCAcg                                                                                            <  2:306167/140‑1 (MQ=255)                                         cgTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCAcg                                                                                            >  1:94623/1‑140 (MQ=255)                                         cgTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCAcg                                                                                            >  1:449164/1‑140 (MQ=255)                                            aTTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCAcgccg                                                                                         <  2:973751/140‑1 (MQ=255)                                              ttACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCcgcg                                                                                       <  2:1000393/140‑1 (MQ=255)                                                 cGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAAt                                                                                    <  2:449164/140‑1 (MQ=255)                                                    cACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATcac                                                                                 <  2:596226/140‑1 (MQ=255)                                                      cGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATcacca                                                                               >  1:345935/1‑140 (MQ=255)                                                       ggTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATcaccac                                                                              >  2:958769/1‑140 (MQ=255)                                                           aGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGGCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGCGCCGGCCAGGTAAATTGGTCCGCACCGGTCACAGCCCCGGCCGGCGTCACCAcatca                                                                          >  2:477576/1‑140 (MQ=255)                                                             cAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACatcatc                                                                        <  2:1124294/140‑1 (MQ=255)                                                                 tACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCaaaa                                                                    >  2:57003/1‑140 (MQ=255)                                                                     tttttCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCaa                                                                <  1:988290/140‑1 (MQ=255)                                                                          ccTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTg                                                           <  1:958769/140‑1 (MQ=255)                                                                          ccTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTg                                                           >  2:1092783/1‑140 (MQ=255)                                                                          ccTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTg                                                           >  2:217804/1‑140 (MQ=255)                                                                           cTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGa                                                          >  2:144888/1‑140 (MQ=255)                                                                              aGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGatat                                                       <  1:1092783/140‑1 (MQ=255)                                                                                ttttCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTg                                                     >  1:359806/1‑140 (MQ=255)                                                                                  ttCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGcc                                                   <  1:1144999/140‑1 (MQ=255)                                                                                    cACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGt                                                 <  1:57003/140‑1 (MQ=255)                                                                                       ttCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCtt                                              <  2:359806/140‑1 (MQ=255)                                                                                        tCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCttt                                             >  2:994926/1‑140 (MQ=255)                                                                                        tCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCttt                                             >  2:226502/1‑140 (MQ=255)                                                                                            gTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTctct                                         >  1:929898/1‑140 (MQ=255)                                                                                            gTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTctct                                         <  2:929898/140‑1 (MQ=255)                                                                                            gTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTctct                                         <  1:614248/140‑1 (MQ=255)                                                                                                         tgCCGCCTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGtt                            >  1:1000902/1‑140 (MQ=255)                                                                                                            cgccTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTca                         <  1:144888/140‑1 (MQ=255)                                                                                                               cTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTcagca                      <  1:217804/140‑1 (MQ=255)                                                                                                               cTGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTcagca                      <  1:226502/140‑1 (MQ=255)                                                                                                                tGCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTcagcag                     >  1:23848/1‑140 (MQ=255)                                                                                                                 gCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGa                    <  1:994926/140‑1 (MQ=255)                                                                                                                 gCGGCTGAGCTGATACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGa                    >  1:930886/1‑140 (MQ=255)                                                                                                                     ctgagctgaTACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGATTaa                <  1:960815/140‑1 (MQ=255)                                                                                                                             aTACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGATTAACGAGGttt        >  1:91866/1‑140 (MQ=255)                                                                                                                              tACCTGAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTCCTCTTGGCACAACAGTTTCAGCAGATTAACGAGGTTtg       <  2:573024/140‑1 (MQ=255)                                                                                                                                   gAATAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGATTAACGAGGTTTGTGCCg  >  2:423867/1‑140 (MQ=255)                                                                                                                                    aaTAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGATTAACGAGGTTtg       <  2:894438/134‑1 (MQ=255)                                                                                                                                    aaTAGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGATTAACGAGGTTtg       >  1:894438/1‑134 (MQ=255)                                                                                                                                       |                                                                                                                                        GCCATCCAGCCAAAAAGAATGATTGCCAGCGCCATCAACGCGTATTTACGCCACGGTGAGCAGGTACATTTTTCCTCAGTTTTCACTTCCGGTGCCGCTTTTTGTGCCGCCTGCGGCTGAGCTGATACCTGAATCGGCGGTGCCGGCCAGGTAATTTCGCCCGCACGGATCACGGTCACGCCGCGAATCACCACATCATCAAAATCAACAGTGATATTGCCGTCTTTCTCTTTGCACAACAGTTTCAGCAGATTAACGAGGTTTGTGCCG  >  NC_000913/1676654‑1676923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑