Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,505,038	G→T	A348E (GCG→GAG)	ydcO ←	putative transport protein YdcO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,505,038	0	G	T	100.0%	164.6 / NA	48	A348E (GCG→GAG)	ydcO	putative transport protein YdcO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (26/22);  total (26/22)

TATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCGCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCACTACCAAACA  >  NC_000913/1504909‑1505173                                                                                                                                  |                                                                                                                                        tatCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTcgctc                                                                                                                               >  2:143431/1‑140 (MQ=255)     ggTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTcgctcacgc                                                                                                                           <  1:315221/140‑1 (MQ=255)      gTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTcgctcacgct                                                                                                                          >  1:494260/1‑140 (MQ=255)      gTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTcgctcacgct                                                                                                                          <  2:1079194/140‑1 (MQ=255)           tGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTa                                                                                                                     >  2:1226056/1‑140 (MQ=255)           tGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTa                                                                                                                     >  1:1195634/1‑140 (MQ=255)               aGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCa                                                                                                                 <  1:417774/140‑1 (MQ=255)                 cGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAgc                                                                                                               >  2:1232943/1‑140 (MQ=255)                   aTTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAgcgc                                                                                                             <  1:487922/140‑1 (MQ=255)                           cAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACa                                                                                                     <  1:143431/140‑1 (MQ=255)                              caccacGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAAc                                                                                                  >  1:376303/1‑140 (MQ=255)                               accacGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACt                                                                                                 >  2:386/1‑140 (MQ=255)                                   cGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTgccg                                                                                             >  2:762144/1‑140 (MQ=255)                                   cGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTgccg                                                                                             >  1:468604/1‑140 (MQ=255)                                       aCAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGa                                                                                         <  2:1195634/140‑1 (MQ=255)                                       aCAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGa                                                                                         <  2:865984/140‑1 (MQ=255)                                       aCAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGa                                                                                         <  1:386/140‑1 (MQ=255)                                       aCAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGa                                                                                         <  1:1232943/140‑1 (MQ=255)                                           aCGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGTTACAAACTGCCGCCGATGGt                                                                                     >  1:800416/1‑140 (MQ=255)                                             gCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTAc                                                                                   >  1:1462021/1‑140 (MQ=255)                                              ccTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACt                                                                                  >  2:93383/1‑140 (MQ=255)                                                 ccGGCAATTAATCCCCAAAACGCAGAACCAATCACGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTaa                                                                               >  1:810355/1‑140 (MQ=255)                                                  cGGCAATTAACCCCCAAAACGCAAAACCAACCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCCCCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAAc                                                                              <  2:1179421/140‑1 (MQ=255)                                                                      ccAGACCCAACCCCCACCACCTTCAACCCTCTTGCCTTTCCAAAAAATCCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCAt                                                          <  2:1462021/139‑1 (MQ=255)                                                                          aaCCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCACCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTgg                                                      >  1:1083719/1‑140 (MQ=255)                                                                              aagcccGCCCAGCGTCAATCCACTTGCGGTTCCCAGAAATGCCACCCCCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATcc                                                  <  1:45158/137‑1 (MQ=255)                                                                              aaTCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCCCCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATcc                                                  <  1:472661/140‑1 (MQ=255)                                                                                tCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCaa                                                >  1:553268/1‑140 (MQ=255)                                                                                   cGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCCCCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACtt                                             <  1:118252/140‑1 (MQ=255)                                                                                    gACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTa                                            >  1:1075650/1‑140 (MQ=255)                                                                                    gACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTa                                            >  1:295955/1‑140 (MQ=255)                                                                                          cGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACgggca                                      >  1:1517332/1‑140 (MQ=255)                                                                                          cGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGGACGTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACtggca                                      >  2:192447/1‑140 (MQ=255)                                                                                             cAACCCACTGGCCTTTACCAGAAATGCCCCCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACgggcaggg                                   <  1:238163/140‑1 (MQ=255)                                                                                               acccACTTCCCGTTACCAGAAATGCCCCCCCCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACgggcagggca                                 <  2:810355/138‑1 (MQ=255)                                                                                               aTCCACTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACgggcagggca                                 >  2:1359211/1‑140 (MQ=255)                                                                                                    cTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCcat                            >  1:147330/1‑140 (MQ=255)                                                                                                    cTTGCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCcat                            <  1:1226056/140‑1 (MQ=255)                                                                                                       gCCGTTACCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCcatcat                         >  1:923674/1‑140 (MQ=255)                                                                                                             aCCAGAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGt                   >  1:251673/1‑140 (MQ=255)                                                                                                               cggAAATGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTaa                 >  2:1022945/3‑140 (MQ=255)                                                                                                                  aaaTGCCCCCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATgg              <  1:1359211/140‑1 (MQ=255)                                                                                                                  aaaTGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGTTACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATgg              <  2:800416/140‑1 (MQ=255)                                                                                                                  aaaTGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATgg              <  2:295955/140‑1 (MQ=255)                                                                                                                    aTGCCCCCCCCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCa            <  1:979544/140‑1 (MQ=255)                                                                                                                    aTGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCa            <  2:494260/140‑1 (MQ=255)                                                                                                                     tGCCACCACCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCAc           >  2:428193/1‑140 (MQ=255)                                                                                                                       ccaccaCCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCACTa         >  2:782369/1‑140 (MQ=255)                                                                                                                        caccacCGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCACTAc        >  1:186799/1‑140 (MQ=255)                                                                                                                            accGCCTCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCACTACCaaa    <  2:251673/140‑1 (MQ=255)                                                                                                                              cgcctcGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCACTACCAAACa  >  1:1296297/1‑140 (MQ=255)                                                                                                                                  |                                                                                                                                        TATCGGTTTCTGTCAGCGATTAAATTCAACACCACGTAACAAACGCCTCCGGCAATTAATCCCCAAAACGCAGAACCAATCCCGACCAGCGTCAATCCACTTGCCGTTACCAGAAATGCCACCACCGCCGCGTCTCGCTCACGCTCATTATGCAGCGCCTGATACAAACTGCCGCCGATGGTACTTAACAGCGCCAGACCTGCCAGCATCTGGATCCAACTTACGGGCAGGGCAGCCATCATCCCGGTAATGGCACTACCAAACA  >  NC_000913/1504909‑1505173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑