Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,140,133	G→T	L697M (CTG→ATG)	ptsA ←	putative PTS multiphosphoryl transfer protein PtsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,140,133	0	G	T	100.0%	320.1 / NA	90	L697M (CTG→ATG)	ptsA	putative PTS multiphosphoryl transfer protein PtsA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/51);  total (39/51)

GACCGGTAGAGAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCACGGCTA  >  NC_000913/4139998‑4140263                                                                                                                                        |                                                                                                                                   gACCGGTAGAGAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATtgtg                                                                                                                                <  1:1195901/140‑1 (MQ=255) gACCGGTAGAGAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATtgtg                                                                                                                                >  1:919295/1‑140 (MQ=255) gACCGGTAGAGAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATtgtg                                                                                                                                >  1:743554/1‑140 (MQ=255)      gTAGAGAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCa                                                                                                                           >  1:1681844/1‑140 (MQ=255)       tAGAGAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCAc                                                                                                                          >  2:499655/1‑140 (MQ=255)       tAGAGAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCAc                                                                                                                          >  2:864140/1‑140 (MQ=255)       tAGAAAAACCGGCCTCGCGCGCCCACAAGTCGGCTCCCAGTTTACGCGGATAGCGGCAGCCGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCAc                                                                                                                          <  2:339093/140‑1 (MQ=255)        agagAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACt                                                                                                                         <  2:228599/140‑1 (MQ=255)        agagAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACt                                                                                                                         <  1:864140/140‑1 (MQ=255)           gaAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTcgg                                                                                                                      <  1:521800/140‑1 (MQ=255)           gaAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTcgg                                                                                                                      <  1:1335483/140‑1 (MQ=255)             aaaCGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTcggcg                                                                                                                    <  2:1122485/140‑1 (MQ=255)             aaaCGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTcggcg                                                                                                                    <  2:1168533/140‑1 (MQ=255)             aaaCGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCACGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTcggcg                                                                                                                    <  2:1574208/140‑1 (MQ=255)                cGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTg                                                                                                                 >  2:1086125/1‑140 (MQ=255)                  gCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGAc                                                                                                               <  2:1657854/140‑1 (MQ=255)                    cTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCa                                                                                                             <  2:651204/140‑1 (MQ=255)                         gcgcCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGt                                                                                                        <  2:957187/140‑1 (MQ=255)                         gcgcCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGt                                                                                                        <  1:208948/140‑1 (MQ=255)                            cccACAAGTCGGCTTCCGGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCg                                                                                                     <  2:1651716/140‑1 (MQ=255)                            cccACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCg                                                                                                     <  1:1358902/140‑1 (MQ=255)                            cccACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCg                                                                                                     <  2:1288922/140‑1 (MQ=255)                            cccACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCg                                                                                                     <  2:1544253/140‑1 (MQ=255)                            cccACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCCTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCg                                                                                                     <  1:333016/140‑1 (MQ=255)                             ccACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGt                                                                                                    >  1:1474820/1‑140 (MQ=255)                              cacaAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTc                                                                                                   <  1:1267694/140‑1 (MQ=255)                                   gTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCg                                                                                                     <  2:1160876/133‑1 (MQ=255)                                   gTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCg                                                                                                     >  1:1160876/1‑133 (MQ=255)                                      ggCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTggg                                                                                           <  2:704308/140‑1 (MQ=255)                                           ccAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATg                                                                                      <  1:1345039/140‑1 (MQ=255)                                           ccAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATg                                                                                      <  1:1285794/140‑1 (MQ=255)                                              gTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCgg                                                                                   >  2:1401240/1‑140 (MQ=255)                                                  aCGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAAtt                                                                               <  1:1611748/140‑1 (MQ=255)                                                       gATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGcc                                                                          <  1:993452/140‑1 (MQ=255)                                                         tAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCcag                                                                        >  1:605201/1‑140 (MQ=255)                                                            gggcggcggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCcagcag                                                                     <  1:1540400/139‑1 (MQ=255)                                                            cggcagcggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCcagcag                                                                     <  2:21148/140‑1 (MQ=255)                                                            cggcagcggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCcagcag                                                                     <  2:919295/140‑1 (MQ=255)                                                             ggcagcggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAgg                                                                    <  1:856357/140‑1 (MQ=255)                                                             ggcagcggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAgg                                                                    <  2:224197/140‑1 (MQ=255)                                                                  cggcCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTc                                                               >  1:848892/1‑140 (MQ=255)                                                                      ccGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACt                                                           >  2:1695305/1‑140 (MQ=255)                                                                           agcagcagGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAg                                                      <  1:1523265/140‑1 (MQ=255)                                                                                      aTCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGc                                           <  1:622314/140‑1 (MQ=255)                                                                                       tCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGc                                           >  1:1298998/1‑139 (MQ=255)                                                                                       tCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGc                                           <  2:1298998/139‑1 (MQ=255)                                                                                          gTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGc                                       >  2:66701/1‑140 (MQ=255)                                                                                           tCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGcc                                      >  1:465229/1‑140 (MQ=255)                                                                                           tCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGcc                                      >  2:632974/1‑140 (MQ=255)                                                                                            cATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCa                                     <  1:761969/140‑1 (MQ=255)                                                                                             aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCGTGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAt                                    >  2:1437176/1‑140 (MQ=255)                                                                                             aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCa                                     >  2:1485236/1‑139 (MQ=255)                                                                                             aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCa                                     <  1:1485236/139‑1 (MQ=255)                                                                                             aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAt                                    >  1:809013/1‑140 (MQ=255)                                                                                             aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAt                                    >  1:728937/1‑140 (MQ=255)                                                                                             aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAt                                    >  1:1625322/1‑140 (MQ=255)                                                                                             aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAt                                    >  2:129446/1‑140 (MQ=255)                                                                                             aTGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAt                                    >  1:1205734/1‑140 (MQ=255)                                                                                              tGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAtt                                   >  1:368993/1‑140 (MQ=255)                                                                                              tGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAtt                                   >  1:1296971/1‑140 (MQ=255)                                                                                              tGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCAtt                                   >  2:1405347/1‑140 (MQ=255)                                                                                               gCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTg                                  <  1:632974/140‑1 (MQ=255)                                                                                               gCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTg                                  <  2:848892/140‑1 (MQ=255)                                                                                               gCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTg                                  <  1:1025013/140‑1 (MQ=255)                                                                                                 cTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGTGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGcc                                <  1:1086125/140‑1 (MQ=255)                                                                                                 cTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGcc                                <  1:66701/140‑1 (MQ=255)                                                                                                    tGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTgg                             >  1:372538/1‑140 (MQ=255)                                                                                                        cACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGc                                           <  2:940333/122‑1 (MQ=255)                                                                                                        cACTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGc                                           >  1:940333/1‑122 (MQ=255)                                                                                                         aCTTCTTCTTTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAg                        <  1:1695305/140‑1 (MQ=255)                                                                                                           ttcttcttTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCa                      >  2:572425/1‑140 (MQ=255)                                                                                                           ttcttcttTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCa                      >  1:1272482/1‑140 (MQ=255)                                                                                                           ttcttcttTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCa                      >  2:1059476/1‑140 (MQ=255)                                                                                                             cttcttTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAAc                    >  1:1309773/1‑140 (MQ=255)                                                                                                             cttcttTGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAAc                    >  2:1239337/1‑140 (MQ=255)                                                                                                                  ttGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCg               >  2:1182949/1‑140 (MQ=255)                                                                                                                  ttGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCg               <  1:173360/140‑1 (MQ=255)                                                                                                                  ttGCTGCGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCg               <  2:728937/140‑1 (MQ=255)                                                                                                                    gctgcGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGc             >  2:463578/1‑140 (MQ=255)                                                                                                                     ctgcGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCa            >  2:190435/1‑140 (MQ=255)                                                                                                                     ctgcGCCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCa            >  2:12234/1‑140 (MQ=255)                                                                                                                        cgcCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCa            <  2:1484997/137‑1 (MQ=255)                                                                                                                        cgcCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCa            >  1:1484997/1‑137 (MQ=255)                                                                                                                         gcCAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCa        <  1:463578/140‑1 (MQ=255)                                                                                                                          ccAGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCAc       <  2:1102518/140‑1 (MQ=255)                                                                                                                            aGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCGGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCACgg     <  2:1681844/140‑1 (MQ=255)                                                                                                                            aGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCACgg     <  2:1272482/140‑1 (MQ=255)                                                                                                                            aGTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCACgg     <  1:572425/140‑1 (MQ=255)                                                                                                                             gTCGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCACGGc    >  1:1272286/1‑140 (MQ=255)                                                                                                                               cGCTTTCCATTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCACGGCTa  <  2:1205734/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   GACCGGTAGAGAAAACGGCCTCGCGCGCCCACAAGTCGGCTTCCAGTTTACGCGGATAGCGGCAGCGGCCCGCCAGCAGCAGGTTATCGGTCATGCCTTTGAGCACTTCTTCTTTGCTGCGCCAGTCGCTTTCCAGTGTGATGCACTCGGCGGTGACCAGCGGTGCGTCTTGTTGGGTCATGCGGAATTGCGCCAGCAGGTGTTCTACTTCCAGCGAAGTACGGCAGGCCATTGCCTGGTTGAGCAACTTGCGGCACTCACGGCTA  >  NC_000913/4139998‑4140263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑